MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. EMBO J. (0261-4189) 2011 imex curation Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. EMBO J. (0261-4189) 2011 imex curation Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. EMBO J. (0261-4189) 2011 imex curation Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. EMBO J. (0261-4189) 2011 imex curation Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Tang CJ., Lin SY., Hsu WB., Lin YN., Wu CT., Lin YC., Chang CW., Wu KS., Tang TK. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation sas6_drome Spindle assembly abnormal protein 6 homolog Sas-6 SAS6 CG15524 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MWPPGSEDSYSAKMDYGKSVVNILPSVEMLVNFNGEMTRSSKRSCLLYAERVDFKELLQLRLTEKSDQRRMYITTVDSASFQDLKQDQSLNVSFSGFIDNVVRMLKDCQSGKLELHLTTRDQNLSSGREVHDYYLQFVEIRSFKNLVHLSLPCRSAPLNTVLFYINSMLEASHKKQYILEQSMQQMQAEINAQRAHAERLTTENTNIREALAENTRILEEKHAAEVHQYQEKLSKINEQRSNELERNRRAISGFQAQLDKASLEKAELKSAQEQAEKRCQTLSEELSCCKARVCTLKEQNDKLHGDVANIRKHERKLEYKIEDLKQHTVELQEHIQKGNKEKANIAAELEAEKKILHTKRQALEMASEEISKANQIIVKQSQELLNLKKTIAWRTEVALQQEKAVQAKESLLSLRENELREARITIEKLREEIPQQLQSMRNFAQGLEQKYSKQILILKERLAIPTGKENRR DIP protein Q9VAC8 original sequence version: 75 F30EB92E059BDDB0 q9xz31_drome ana2 Ana-2 Ana2 BcDNA:LD22033 Dmel\CG8262 STIL CG8262 Dmel_CG8262 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFVPETEDMLPRLAPRPSAAVPMGHTNEIIGPTVPEVSILFGQPPQDPQMQPQQPNHAHQESSPRAAPTFASWKKQMLPRVNFSPILATELGPRTAPSSNSPCSSKEYQVMPRDRNYASDAQQTTNHMNCNAGGYNAEPKQQAYQSRRLSSSSNADVLTICAGTQTDPFNPSPPRKSLPQVVYSDIDVSNLANKSDLAALVSLVESVRHEQQQLRNLCEMILEQQQRAKPEAISKASSTTGTQCDILTTNQAGRRLSPIMQDYIAEEEEPLPPPQARVIPQFPSPRPHPPIAQSTGYRANTPQAKRIPQLAKPNTEKSMVMNELALKYLRQPVDELMKDMRLGASPKSPNPEPLRPIDNIGHAQSPNDISNASYKYLKKYRLLPEEQLEPVCPQSPMAATAPSPHIQLDLENIRNQPKLL DIP protein Q9XZ31 original sequence version: 104 34FD27B261B275AA cenpj_human Centromere protein J CENPJ CPAP LAP LIP1 Centrosomal P4.1-associated protein LAG-3-associated protein LYST-interacting protein 1 protein protein human Homo sapiens Human MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTEL DIP protein Q9HC77 original sequence version: 70 DIP protein Q9HC77 original sequence version: 99 B4E6531B62A30F2D stil_human SCL-interrupting locus protein STIL SIL TAL-1-interrupting locus protein protein protein human Homo sapiens Human MEPIYPFARPQMNTRFPSSRMVPFHFPPSKCALWNPTPTGDFIYLHLSYYRNPKLVVTEKTIRLAYRHAKQNKKNSSCFLLGSLTADEDEEGVTLTVDRFDPGREVPECLEITPTASLPGDFLIPCKVHTQELCSREMIVHSVDDFSSALKALQCHICSKDSLDCGKLLSLRVHITSRESLDSVEFDLHWAAVTLANNFKCTPVKPIPIIPTALARNLSSNLNISQVQGTYKYGYLTMDETRKLLLLLESDPKVYSLPLVGIWLSGITHIYSPQVWACCLRYIFNSSVQERVFSESGNFIIVLYSMTHKEPEFYECFPCDGKIPDFRFQLLTSKETLHLFKNVEPPDKNPIRCELSAESQNAETEFFSKASKNFSIKRSSQKLSSGKMPIHDHDSGVEDEDFSPRPIPSPHPVSQKISKIQPSVPELSLVLDGNFIESNPLPTPLEMVNNENPPLINHLEHLKPLQPQLYDEKHSPEVEAGEPSLRGIPNQLNQDKPALLRHCKVRQPPAYKKGNPHTRNSIKPSSHNGPSHDIFEKLQTVSAGNVQNEEYPIRPSTLNSRQSSLAPQSQPHDFVFSPHNSGRPMELQIPTPPLPSYCSTNVCRCCQHHSHIQYSPLNSWQGANTVGSIQDVQSEALQKHSLFHPSGCPALYCNAFCSSSSPIALRPQGDMGSCSPHSNIEPSPVARPPSHMDLCNPQPCTVCMHTPKTESDNGMMGLSPDAYRFLTEQDRQLRLLQAQIQRLLEAQSLMPCSPKTTAVEDTVQAGRQMELVSVEAQSSPGLHMRKGVSIAVSTGASLFWNAAGEDQEPDSQMKQDDTKISSEDMNFSVDINNEVTSLPGSASSLKAVDIPSFEESNIAVEEEFNQPLSVSNSSLVVRKEPDVPVFFPSGQLAESVSMCLQTGPTGGASNNSETSEEPKIEHVMQPLLHQPSDNQKIYQDLLGQVNHLLNSSSKETEQPSTKAVIISHECTRTQNVYHTKKKTHHSRLVDKDCVLNATLKQLRSLGVKIDSPTKVKKNAHNVDHASVLACISPEAVISGLNCMSFANVGMSGLSPNGVDLSMEANAIALKYLNENQLSQLSVTRSNQNNCDPFSLLHINTDRSTVGLSLISPNNMSFATKKYMKRYGLLQSSDNSEDEEEPPDNADSKSEYLLNQNLRSIPEQLGGQKEPSKNDHEIINCSNCESVGTNADTPVLRNITNEVLQTKAKQQLTEKPAFLVKNLKPSPAVNLRTGKAEFTQHPEKENEGDITIFPESLQPSETLKQMNSMNSVGTFLDVKRLRQLPKLF DIP protein Q15468 original sequence version: 93 mint homomint 7F15BEDA8717659C sas6_human Spindle assembly abnormal protein 6 homolog SASS6 SAS6 protein protein human Homo sapiens Human MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRKDLVIRLTDDTDPFFLYNLVISEEDFQSLKFQQGLLVDFLAFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILDNSPAFLNVVETNPFKHLTHLSLKLLPGNDVEIKKFLAGCLKCSKEEKLSLMQSLDDATKQLDFTRKTLAEKKQELDKLRNEWASHTAALTNKHSQELTNEKEKALQAQVQYQQQHEQQKKDLEILHQQNIHQLQNRLSELEAANKDLTERKYKGDSTIRELKAKLSGVEEELQRTKQEVLSLRRENSTLDVECHEKEKHVNQLQTKVAVLEQEIKDKDQLVLRTKEAFDTIQEQKVVLEENGEKNQVQLGKLEATIKSLSAELLKANEIIKKLQGDLKTLMGKLKLKNTVTIQQEKLLAEKEEKLQKEQKELQDVGQSLRIKEQEVCKLQEQLEATVKKLEESKQLLKNNEKLITWLNKELNENQLVRKQDVLGPSTTPPAHSSSNTIRSGISPNLNVVDGRLTYPTCGIGYPVSSAFAFQNTFPHSISAKNTSHPGSGTKVQFNLQFTKPNASLGDVQSGATISMPCSTDKENGENVGLESKYLKKREDSIPLRGLSQNLFSNSDHQRDGTLGALHTSSKPTALPSASSAYFPGQLPNS 6D19696BC748849F q9vi72_drome CPAP D-Sas4 d-SAS-4 dsas-4 D-sas4 DSAS-4 DSas-4 DSas4 Dmel\CG10061 Dsas-4 Dsas4 SAS-4 SAS4 Sas4 l(3)S2214 l(3)s2214 sas-4 sas4 Dmel_CG10061 Sas-4 CG10061 DmSas4 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQEAGESPVGMPLSQEAIAQRLAALSRWQDEQKRLLQERQSNHRVLLGLEQRNMYKMLGLLHQETESHENSVLEEWDEEHSIQMPRLAADPDEHEPEISMADPQPAKPKRPFLRRGEGLKQRFKINPDQLRLENLPRYKFANAHPQFRTPQMKKGILKKQVKSPPNPAPPPAPKPSAQLDDLNEQRFQQMLIGKNACSSTPDLKSSYASSTTASSTSPRVRFVEQKSRAGQTTHADDEASSEASPMTGVCWAKVLDTQSIKPAQIQRRSAQIRVEDDSNVSIFELLEQKATEGNIDMNSSCIRTFMARKDQRRRVADDSDHIVVTQQVRQMRLQPQVDQMLVQELQEGDEEDTEPSSDQTLTMTPIQVGNTKVRVRFSDSNDTHEYSDATSLNDGSNIQLFEQFKSALFQALEQKKKSSPSKESDEPITKDLQEKANLVRTRLEELETEIATFKEQNAQLLRLRQQHELEKAKCTQDHMEAMERVHDEKIQAEIYLHDERMKIEEERRKFEQQMRLQKSNANSKEKKEIAALKQEVEGLQLQLKQKEQAHVSAQARLRAQLRASEKEQRNYRDEIELLRKENKRLEQELVKIGRENNSKMLQEINRNIARLAPKVLPSATMSDILDENGRRTQSLDGTAGKPAKQREAQNRRQSSGSAQVRSRSRSLSRNKKTPYALDESYASSSSVESEEVQPPVTAPKAATPPPAANSSSDFKREITNADGSKDIWYPNGNLKKISADGMNLRMLYFNKDIKETNIREGTVKYYYAETNTWHTSYLDGLEILEFPNGQTEHRRKDGTVEIHFPNNSIKIVDPSDTEKLEEWRYADGTHLVQLRNGDKILNLPNGQKEIHTKLNKRREYPDGTVKLVYPDGSQETRYSNGRVRLKDKDGKLIMDTDYAKY DIP protein Q9VI72 original sequence version: 64 DIP protein Q9VI72 original sequence version: 92 8A0765CA0A92E492 cenpj-sass6 3 hSAS6 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level STIL 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level CPAP 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f4c homomint mint cenpj-stil 3 STIL 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level CPAP 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9HC77:p.Glu1235Val mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E V Tue Jul 12 10:05:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 20:12:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1235val' Tue Jun 30 20:00:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1235Val' binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association f4e homomint mint cenpj-sass6-1 3 hSAS6 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint sf7b sass6-cenpj 3 hSAS6 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint sf7a stil-cenpj 4 STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4b homomint mint stil-stil 4 STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4b homomint mint stil-sass6 4 hSAS6 9 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4b homomint mint stil-cenpj-1 4 STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q9HC77:p.Glu1235Val mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E V Tue Jul 12 10:09:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 18:17:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1235val' Tue Jun 30 18:27:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1235Val' gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4d homomint mint cenpj-stil-1 4 STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q9HC77:p.Glu1235Val mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E V Tue Jul 12 10:09:39 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:27:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1235val' Tue Jun 30 16:22:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1235Val' gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4d homomint mint stil-cenpj-2 4 hSAS6 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint sf7a stil-cenpj-3 1 STIL 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain CPAP 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate Q9HC77:p.Glu1235Val mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E V Tue Jul 12 10:12:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:13:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1235val' Tue Jun 30 17:19:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1235Val' gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4f homomint mint cenpj-stil-2 1 STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association homomint mint sf5b cenpj-stil-3 1 STIL 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CPAP 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association homomint mint sf5c cenpj-stil-4 1 STIL 8 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated CPAP 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint mint sf6a ana2-sas_4 1 dSAS4 10 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated Ana2 6 weight by staining molecular weight estimation by staining weight by staining predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction mint sf4b ana2-sas_6 1 Ana2 6 weight by staining molecular weight estimation by staining weight by staining predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined dSAS6 5 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated direct interaction direct interaction mint sf4b cenpj-stil-5 2 STIL 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain CPAP 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9HC77:p.Glu1235Val mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E V Tue Jul 12 10:54:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 14:58:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1235val' Tue Jun 30 19:50:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1235Val' physical association physical association homomint mint sf6b sf8