matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. Matrix Biol. (0945-053X) 2012 imex curation Lin L., Betsuyaku T., Heimbach L., Li N., Rubenstein D., Shapiro S.D., An L., Giudice G.J., Diaz L.A., Senior R.M., Liu Z. zhiliu@med.unc.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Lin L., Betsuyaku T., Heimbach L., Li N., Rubenstein D., Shapiro S.D., An L., Giudice G.J., Diaz L.A., Senior R.M., Liu Z. zhiliu@med;unc.edu zhiliu@med.unc.edu Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Matrix Biol. (0945-053X) 2012 Only protein-protein interactions imex curation Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. Matrix Biol. (0945-053X) 2012 imex curation Lin L., Betsuyaku T., Heimbach L., Li N., Rubenstein D., Shapiro S.D., An L., Giudice G.J., Diaz L.A., Senior R.M., Liu Z. zhiliu@med.unc.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Lin L., Betsuyaku T., Heimbach L., Li N., Rubenstein D., Shapiro S.D., An L., Giudice G.J., Diaz L.A., Senior R.M., Liu Z. zhiliu@med;unc.edu zhiliu@med.unc.edu Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Matrix Biol. (0945-053X) 2012 Only protein-protein interactions imex curation coha1_mouse Collagen alpha-1(XVII) chain Col17a1 Bp180 Bpag2 180 kDa bullous pemphigoid antigen 2 Bullous pemphigoid antigen 2 protein protein mouse Mus musculus Mouse MDVTKKSKRDGTEVTERIVTETVTTRLTSLPPKGSTSNGYAKTGSLGGGSRLEKQSLTHGSSGYINSSGSIRGNASTSSYRRTHSPASTLPNSPGSTFERKAHMTRHGTYEGSSSGNSSPEYPRKELASSSTRGRSQTRESEIRVRLQSASPSTRWTELDEVKRLLKGSRSASASPTRNTSNTLPIPKKGTVETKTVTASSHSVSGTYDSAILDTNFPPHMWSSTLPAGSSLGTYQNNITAQSTSLLNTNAYSTGSVFGVPNNMASCSPTLHPGLSSCSSVFGMQNNLAPSSSVLSHGTTTASTAYGAKKNVPQPPTVTSTGVSTSATCTTSVQSDDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPATISSTSFSEDTLKKEKQAAYAADTCLKADVNGDLNTVSTKSKMTSAENHGYDRGGGGGRGKGGGAGGGGGGGGASGGGGAWGAAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRKLKARVEELEKTKVLYHDVQMDKSNRDRLQAEAPSLGPGLGKAELDGYSQEAIWLFVRNKLMTEQENGNLRGSPGPKGDMGSQGPKGDRGLPGTPGIPGPLGHPGPEGPKGQKGSIGDPGMEGPIGQRGLAGPMGPRGEPGPPGSGEKGDRGIAGEQGPQGLPGVPGPPGLRGHSGSPGPQGPPGAVGPQGLRGDVGLPGVKGDKGLMGPPGPKGDQGEKGPRGLTGEPGIRGLPGAVGEPGAKGAMGPAGADGQQGSRGEQGLTGMPGTRGPPGPAGDPGKPGLTGPQGPQGLPGSPGRPGTKGEPGAPGRVMTSEGSSTITVPGPPGPPGAMGPPGPPGTPGPAGPAGLPGQQGPRGEPGLAGDSFLSSGSSISEVLSAQGVDLRGPPGPPGPRGPPGPSIPGPPGPRGPPGEGVPGPPGPPGSFLTDSETFFTGPPGPPGPPGPKGDQGDPGVPGTPGISGGLSHGASSSTLYMQGPPGPPGPPGPPGSLSSSGQDIQHYIAEYMQSDNIRTYLSGVQGPPGPPGPPGPVITITGETFDYSQLASQVVSYLRSSGYGAGLSSASSSEDILAMLRRNDVWQYLRQNLVGPPGPPGPPGVSGDGSLLSLDYGELSRHILNYMSSSGISFGHPGPPGPPGLPGTSYEELLTMLRGSDYRNIIGPPGPPGPPGMPGNAWSSISVEDLSSYLHTAGLSSIPGPPGPPGPPGPRGPPGVSAALSTYAAENSDNFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDGHLRENYNWSSNSSARRGTSYSSSTGTGGTNGGSLGEGGAYGAGDGGPYGTDIGPGGGYGAAAGGGIYGTNGDSFRDGFTGDLDYNKLAVRVSESMQRQGLLQGMAYTVQGPPGPQGPPGISRVFSAYSNVTQDLMDFFQTYGTIPGPPGQKGDVGTPGPKGDRGPAGPRGPPGPPGPRGNKGEKGDKGDQVYTGRRKRSIAIKP 7C38356D6ED68347 elne_human Neutrophil elastase Elastase-2 ELANE PMN elastase Bone marrow serine protease Medullasin Human leukocyte elastase ELA2 protein protein human Homo sapiens Human MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH 3F7610DC33CAA4B9 col17a1-elane 1 3 enzyme target enzyme target substrate substrate neutral component neutral component tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sequence required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 enzyme enzyme neutral component neutral component human-neutrophil Homo sapiens polymorphonuclear neutrophil neutrophil Neutrophil [Neutrophilic granulocyte] protein cleavage protein cleavage 1A/1C col17a1-elane-7 2 3 enzyme target enzyme target substrate substrate neutral component neutral component binding site required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 enzyme enzyme neutral component neutral component human-neutrophil Homo sapiens polymorphonuclear neutrophil neutrophil Neutrophil [Neutrophilic granulocyte] protein cleavage protein cleavage 1B