IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma pull down pull down anti tag western anti tag western blot anti tag western Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions human-nci_h460 Human NCI-H460 large cell lung carcinoma cell line nci_h460 Human NCI-H460 large cell lung carcinoma cell line ATCC =HTB-177 http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=HTB-177&Template=cellBiology confocal microscopy confocal microscopy immunostaining immunostaining Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma pull down pull down western blot western blot Immuno blot Immuno blot Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions mouse-gd25 Mouse GD25 embryonic fibroblasts, integrin beta1 null. gd25 Mouse GD25 embryonic fibroblasts, integrin beta1 null. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation SHARPIN is an endogenous inhibitor of β1-integrin activation. SHARPIN is an endogenous inhibitor of β1-integrin activation. Nat. Cell Biol. (1465-7392) 2011 imex curation Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Rantala J.K., Pouwels J., Pellinen T., Veltel S., Laasola P., Mattila E., Potter C.S., Duffy T., Sundberg J.P., Kallioniemi O., Askari J.A., Humphries M.J., Parsons M., Salmi M., Ivaska J. fig 4d - antibody is PAN- kindlin - nbg, as immunogen seq is not even given -probably not known by supplier anyway. Integrin-beta1 knockout mefs. Species of alpha5-gfp, mcherry-kindlin amd mcherry-talin confirmed by the authors. Figure 4d (PLA) has not been included as the identity of the target Kindlin molecule(s) is not known. Johanna.ivaska@vtt.fi Accepted 2012-FEB-27 by ORCHARD Nat. Cell Biol. (1465-7392) 2011 Only protein-protein interactions imex curation itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C ita1_human Integrin alpha-1 ITGA1 Laminin and collagen receptor VLA-1 CD49 antigen-like family member A protein protein human Homo sapiens Human MAPRPRARPGVAVACCWLLTVVLRCCVSFNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINATVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK DIP protein P56199 original sequence version: 85 1A86F30FD916C845 itb1_mouse Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta Itgb1 protein protein mouse Mus musculus Mouse MNLQLVSWIGLISLICSVFGQTDKNRCLKANAKSCGECIQAGPNCGWCTNTTFLQEGMPTSARCDDLEALKKKGCQPSDIENPRGSQTIKKNKNVTNRSKGMAEKLRPEDITQIQPQQLLLKLRSGEPQKFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTDRGEFFNELVGQQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSGNSSNVIQLIIDAYNSLSSEVILENSKLPDGVTINYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITANKCPNKESETIKIKPLGFTEEVEVVLQFICKCNCQSHGIPASPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCRCRVCECYPNYTGSACDCSLDTGPCLASNGQICNGRGICECGACKCTDPKFQGPTCETCQTCLGVCAEHKECVQCRAFNKGEKKDTCAQECSHFNLTKVESREKLPQPVQVDPVTHCKEKDIDDCWFYFTYSVNGNNEAIVHVVETPDCPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P09055 original sequence version: 100 26788F7F0A168B56 ferm2_mouse Fermitin family homolog 2 Fermt2 Plekhc1 Kindlin-2 Pleckstrin homology domain-containing family C member 1 protein protein mouse Mus musculus Mouse MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKCGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLIMPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPVTSPEILAKMFKPQALLDKAKTNQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWALLLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSREESSGTPAHQLNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSFLKMQHLNPDPQLIPDQITTDVNPECLVSPRYLKKYKSKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKREELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV 89767FA0E34B543B il1a_human Interleukin-1 alpha IL1A IL1F1 Hematopoietin-1 protein protein human Homo sapiens Human MAKVPDMFEDLKNCYSENEEDSSSIDHLSLNQKSFYHVSYGPLHEGCMDQSVSLSISETSKTSKLTFKESMVVVATNGKVLKKRRLSLSQSITDDDLEAIANDSEEEIIKPRSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA DIP protein P01583 original sequence version: 168 CBCCC49569D9ED40 shrpn_rat Sharpin Sharpin Shank-associated RH domain-interacting protein protein protein rat Rattus norvegicus (Rat) Rat MSPPAGGAAAAADPASPVVLLAVQAAVRLLGAGHEDEAQLRKLQLKADPERPGRFRLGLLGIEPGAVSLEWPLESICYTIRGPNQHELQPPPGGPGTFSVHFLNSEEAQQWAALVRDATAEGQNGNDSTAPVPTPAMCPTSPPCSSVTPTPKATQPEMDLPQGSGNLKKEELATHLAQAIAGGDEKAAAQVAAILAQHHVALNVQLLEAWFPRGPIRLQVTVEDATSVLSSSSSAHVSLQIHPHCSIAALQEQVFSEFGFPPAVQRWVIGRCLCMPERSLASYGVSQDGDPAFLYLLSAPREVSGHSPQHSKMDRKLGCLFPQSLELPHNLQASSSSLPSPPQPGWSCPSCTFINASNRPGCEMCSTQRPCAWDPLTATST 8D9A7D7D23665567 shrpn_human Sharpin SHARPIN SIPL1 Shank-associated RH domain-interacting protein Shank-interacting protein-like 1 PSEC0216 protein protein human Homo sapiens Human MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFINAPDRPGCEMCSTQRPCTWDPLAAAST DIP protein Q9H0F6 original sequence version: 80 DIP protein Q9H0F6 original sequence version: 89 DIP protein Q9H0F6-1 original sequence version: 68 DIP protein Q9H0F6 original sequence version: 68 DIP protein Q9H0F6-1 original sequence version: 72 DIP protein Q9H0F6 original sequence version: 91 33EE4BF66936AB60 ita5_human Integrin alpha-5 ITGA5 FNRA Fibronectin receptor subunit alpha Integrin alpha-F VLA-5 CD49 antigen-like family member E protein protein human Homo sapiens Human MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA DIP protein P08648 original sequence version: 123 DIP protein P08648 original sequence version: 137 6B4D558D4F739CBA tln1_mouse Talin-1 Tln1 Tln protein protein mouse Mus musculus Mouse MVALSLKISIGNVVKTMQFEPSTMVYDACRMIRERIPEALAGPPNDFGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTMEYRKKQRPLKIRMLDGTVKTIMVDDSKTVTDMLMTICARIGITNHDEYSLVRELMEEKKDEGTGTLRKDKTLLRDEKKMEKLKQKLHTDDELNWLDHGRTLREQGVEEHETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGFQCQIQFGPHNEQKHKAGFLDLKDFLPKEYVKQKGERKIFQAHKNCGQMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKECVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQYNRVGKVEHGSVALPAIMRSGASGPENFQVGSMPPAQQQITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAAQATLDDFETLPPLGQDAASKAWRKNKMDESKHEIHSQVDAITAGTASVVNLTAGDPAETDYTAVGCAVTTISSNLTEMSRGVKLLAALLEDEGGNGRPLLQAAKGLAGAVSELLRSAQPASAEPRQNLLQAAGNVGQASGELLQQIGESDTDPHFQDVLMQLAKAVASAAAALVLKAKSVAQRTEDSGLQTQVIAAATQCALSTSQLVACTKVVAPTISSPVCQEQLVEAGRLVAKAVEGCVSASQAATEDGQLLRGVGAAATAVTQALNELLQHVKAHATGAGPAGRYDQATDTILTVTENIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQRLREAAEGLRMATNAAAQNAIKKKLVQRLEHAAKQAAASATQTIAAAQHAASAPKASAGPQPLLVQSCKAVAEQIPLLVQGVRGSQAQPDSPSAQLALIAASQSFLQPGGKMVAAAKASVPTIQDQASAMQLSQCAKNLGTALAELRTAAQKAQEACGPLEMDSALSVVQNLEKDLQEIKAAARDGKLKPLPGETMEKCTQDLGNSTKAVSSAIAKLLGEIAQGNENYAGIAARDVAGGLRSLAQAARGVAALTSDPAVQAIVLDTASDVLDKASSLIEEAKKASGHPGDPESQQRLAQVAKAVTQALNRCVSCLPGQRDVDNALRAVGDASKRLLSDSLPPSTGTFQEAQSRLNEAAAGLNQAATELVQASRGTPQDLARASGRFGQDFSTFLEAGVEMAGQAPSQEDRAQVVSNLKGISMSSSKLLLAAKALSTDPASPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKECDNALRQLETVRELLENPVQPINDMSYFGCLDSVMENSKVLGEAMTGISQNAKNGNLPEFGDAIATASKALCGFTEAAAQAAYLVGVSDPNSQAGQQGLVEPTQFARANQAIQMACQSLGEPGCTQAQVLSAATIVAKHTSALCNSCRLASARTANPTAKRQFVQSAKEVANSTANLVKTIKALDGDFTEENRAQCRAATAPLLEAVDNLSAFASNPEFSSVPAQISPEGRAAMEPIVISAKTMLESAGGLIQTARALAVNPRDPPRWSVLAGHSRTVSDSIKKLITSMRDKAPGQLECETAIAALNSCLRDLDQASLAAVSQQLAPREGISQEALHTQMLTAVQEISHLIEPLASAARAEASQLGHKVSQMAQYFEPLTLAAVGAASKTLSHPQQMALLDQTKTLAESALQLLYTAKEAGGNPKQAAHTQEALEEAVQMMTEAVEDLTTTLNEAASAAGVVGGMVDSITQAINQLDEGPMGDPEGSFVDYQTTMVRTAKAIAVTVQEMVTKSNTSPEELGPLANQLTSDYGRLASQAKPAAVAAENEEIGAHIKHRVQELGHGCSALVTKAGALQCSPSDVYTKKELIECARRVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNREGAETFADHREGILKTAKVLVEDTKVLVQNAAGSQEKLAQAAQSSVATITRLADVVKLGAASLGAEDPETQVVLINAVKDVAKALGDLISATKAAAGKVGDDPAVWQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATTEHIRQELAVFCSPEPPAKTSTPEDFIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRRAIADMLRACKEAAFHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPNPDLKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEDQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH DIP protein P26039 original sequence version: 109 DIP protein P26039 original sequence version: 114 DIP protein P26039 original sequence version: 151 DIP protein P26039 original sequence version: 165 DIP protein P26039 original sequence version: 136 78832388E2392B8E ita2_human Integrin alpha-2 Platelet membrane glycoprotein Ia Collagen receptor VLA-2 subunit alpha CD49 antigen-like family member B ITGA2 CD49B protein protein human Homo sapiens Human MGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASFSPATQPCPSLIDVVVVCDESNSIYPWDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVTDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQIFSIEGTVQGGDNFQMEMSQVGFSADYSSQNDILMLGAVGAFGWSGTIVQKTSHGHLIFPKQAFDQILQDRNHSSYLGYSVAAISTGESTHFVAGAPRANYTGQIVLYSVNENGNITVIQAHRGDQIGSYFGSVLCSVDVDKDTITDVLLVGAPMYMSDLKKEEGRVYLFTIKKGILGQHQFLEGPEGIENTRFGSAIAALSDINMDGFNDVIVGSPLENQNSGAVYIYNGHQGTIRTKYSQKILGSDGAFRSHLQYFGRSLDGYGDLNGDSITDVSIGAFGQVVQLWSQSIADVAIEASFTPEKITLVNKNAQIILKLCFSAKFRPTKQNNQVAIVYNITLDADGFSSRVTSRGLFKENNERCLQKNMVVNQAQSCPEHIIYIQEPSDVVNSLDLRVDISLENPGTSPALEAYSETAKVFSIPFHKDCGEDGLCISDLVLDVRQIPAAQEQPFIVSNQNKRLTFSVTLKNKRESAYNTGIVVDFSENLFFASFSLPVDGTEVTCQVAASQKSVACDVGYPALKREQQVTFTINFDFNLQNLQNQASLSFQALSESQEENKADNLVNLKIPLLYDAEIHLTRSTNINFYEISSDGNVPSIVHSFEDVGPKFIFSLKVTTGSVPVSMATVIIHIPQYTKEKNPLMYLTGVQTDKAGDISCNADINPLKIGQTSSSVSFKSENFRHTKELNCRTASCSNVTCWLKDVHMKGEYFVNVTTRIWNGTFASSTFQTVQLTAAAEINTYNPEIYVIEDNTVTIPLMIMKPDEKAEVPTGVIIGSIIAGILLLLALVAILWKLGFFKRKYEKMTKNPDEIDETTELSS DIP protein P17301 original sequence version: 108 DIP protein P17301 original sequence version: 186 7E1B7ED968A94070 itga2-sharpin 1 16 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role bait bait conserved_membrane_proximal_segment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig 2b itga1-sharpin-1 1 16 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig 2b SHARPIN associates with the cytoplasmic domain of integrin alpha-subunits. itga2-sharpin-1 4 20 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined conserved_membrane_proximal_segment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 17 unspecified role unspecified role prey prey physical association physical association Fig 2c itga2-sharpin-2 8 17 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role bait bait c_terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig 2d, 2f. itga2-sharpin-4 2 17 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role neutral component neutral component conserved_membrane_proximal_segment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain edans_tag fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P17301:p.Trp1154_Phe1159delinsAlaAlaAlaAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain WKLGFF AAAAAA Wed May 02 15:22:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp_lys_leu_gly_phe_phe1154-1159ala_ala_ala_ala_ala_ala' Tue Jun 30 17:34:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp1154_Phe1159delinsAlaAlaAlaAlaAlaAla' direct interaction direct interaction Fig 2e SHARPIN binding is mainly mediated by residues WK, LG and FF. itga2-sharpin-5 7 20 unspecified role unspecified role bait bait c_terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ilr2alpha_tac_subunit_ec_and tm_domains fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 17 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2g The integrin alpha-subunit binding sequence interacts with SHARPIN even when inserted into an irrelevant transmembrane protein. itga5-sharpin 7 18 unspecified role unspecified role bait bait c_terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ilr2alpha_tac_subunit_ec_and tm_domains fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 17 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2g The integrin alpha-subunit binding sequence interacts with SHARPIN even when inserted into an irrelevant transmembrane protein. itgb1-sharpin-3 3 17 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3 SHARPIN co-localizes with inactive beta1-integrins in cells, but not with active beta1-integrins. itga2-itgb1-2 6 11 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Fig 3c, 3d Using PC3 cells grown on plastic, SHARPIN co-precipitated strongly with alpha2-beta1-integrin and, to a lesser extent, with alpha5-beta1-integrin. SHARPIN coimmunoprecipitated more strongly with alpha-integrins from cells grown in suspension, rather than cells grown adhering to their substrate collagens. itga5-itgb1-1 6 11 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Fig 3c Using PC3 cells grown on plastic, SHARPIN co-precipitated strongly with alpha2-beta1-integrin and, to a lesser extent, with alpha5-beta1-integrin. il1a-sharpin 6 17 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association fig 3e SHARPIN coimmunoprecipitated more strongly with alpha-integrins from cells grown in suspension, rather than cells grown adhering to their substrate collagens. itga5-sharpin 9 17 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P08648:p.Leu1023_Gly1024delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain LG AA Thu Jul 07 12:17:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu1023_gly1024ala Fri Jul 22 11:13:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu1023ala,gly1024ala' Wed May 02 18:45:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_gly1023-1024ala_ala' Tue Jun 30 18:10:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1023_Gly1024delinsAlaAla' physical association physical association fig 3f itga5-sharpin-2 5 18 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role fluorescence donor fluorescence donor region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig 4a itgb1-tln1 10 19 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role fluorescence donor fluorescence donor region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction fig 4b There was a significant reduction in the interaction between beta1–GFP and mCherry–talin and beta1–GFP and mCherry–kindlin-2 in cells positive for MYC–SHARPIN. itgb1-tln1-1 10 11 unspecified role unspecified role fluorescence donor fluorescence donor region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction fig 4c There was a significant reduction in the interaction between beta1–GFP and mCherry–talin and beta1–GFP and mCherry–kindlin-2 in cells positive for MYC–SHARPIN. itgb1-itgb1-3 8 19 unspecified role unspecified role prey prey head_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind less-than less-than certain certain sequence position certain direct interaction direct interaction Fig 2d