IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Plant Mol. Biol. (0167-4412) 2011 imex curation Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu Accepted 2012-APR-24 by MGT 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Plant Mol. Biol. (0167-4412) 2011 Only protein-protein interactions imex curation Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Plant Mol. Biol. (0167-4412) 2011 imex curation Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu Accepted 2012-APR-24 by MGT Arabidopsis thaliana Plant Mol. Biol. (0167-4412) 2011 Only protein-protein interactions imex curation Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Plant Mol. Biol. (0167-4412) 2011 imex curation Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Only protein-protein interactions arath-seedling Arabidopsis thaliana seedling pull down pull down anti tag western anti tag western blot anti tag western Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. 4 day-old dark-grown 35S:His-PIF3-myc seedlings kept in the dark or exposed to continuous blue light (Bc, 25 umol m−2 s−1) for 15 min. huq@mail.utexas.edu Accepted 2012-APR-24 by MGT 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Plant Mol. Biol. (0167-4412) 2011 Only protein-protein interactions imex curation Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Plant Mol. Biol. (0167-4412) 2011 imex curation Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu Accepted 2012-APR-24 by MGT 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Plant Mol. Biol. (0167-4412) 2011 Only protein-protein interactions imex curation Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Plant Mol. Biol. (0167-4412) 2011 imex curation Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. Yeast cells were exposed to a pulse of blue light (3,600 umol m-2), and then incubated in the dark for an additional 3 h before performing a LacZ assay. huq@mail.utexas.edu Accepted 2012-APR-24 by MGT 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Plant Mol. Biol. (0167-4412) 2011 Only protein-protein interactions imex curation Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Dimerization and blue light regulation of PIF1 interacting bHLH proteins in Arabidopsis. Plant Mol. Biol. (0167-4412) 2011 imex curation Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. huq@mail.utexas.edu 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Only protein-protein interactions arath-seedling Arabidopsis thaliana seedling anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bu Q., Castillon A., Chen F., Zhu L., Huq E. EMSA data not added as DNA sequence not given. The TAP tag (containing X9 myc) is recognised by an anti-myc antibody (Shen-2008, Toledo-Ortiz, 2009). huq@mail.utexas.edu Accepted 2012-APR-24 by MGT 20-Jun-2012: Contacted by JYOTI. Arabidopsis thaliana Plant Mol. Biol. (0167-4412) 2011 Only protein-protein interactions imex curation pif4_arath Transcription factor PIF4 Transcription factor EN 102 bHLH transcription factor bHLH009 Basic helix-loop-helix protein 9 Short under red-light 2 PIF4 BHLH9 SRL2 At2g43010 MFL8.13 EN102 Phytochrome-interacting factor 4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEHQGWSFEENYSLSTNRRSIRPQDELVELLWRDGQVVLQSQTHREQTQTQKQDHHEEALRSSTFLEDQETVSWIQYPPDEDPFEPDDFSSHFFSTMDPLQRPTSETVKPKSSPEPPQVMVKPKACPDPPPQVMPPPKFRLTNSSSGIRETEMEQYSVTTVGPSHCGSNPSQNDLDVSMSHDRSKNIEEKLNPNASSSSGGSSGCSFGKDIKEMASGRCITTDRKRKRINHTDESVSLSDAIGNKSNQRSGSNRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSKTDKASILDEAIDYLKSLQLQLQVMWMGSGMAAAAASAPMMFPGVQPQQFIRQIQSPVQLPRFPVMDQSAIQNNPGLVCQNPVQNQIISDRFARYIGGFPHMQAATQMQPMEMLRFSSPAGQQSQQPSSVPTKTTDGSRLDH DIP protein Q8W2F3 original sequence version: 103 DIP protein Q8W2F3-1 original sequence version: 120 B4C081D0BF907C26 pif5_arath Transcription factor PIF5 PIL6 Basic helix-loop-helix protein 65 Phytochrome interacting factor-like 6 F17J16.110 PIF5 EN103 Phytochrome-interacting factor 5 At3g59060 Transcription factor EN 103 BHLH65 bHLH transcription factor bHLH065 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEQVFADWNFEDNFHMSTNKRSIRPEDELVELLWRDGQVVLQSQARREPSVQVQTHKQETLRKPNNIFLDNQETVQKPNYAALDDQETVSWIQYPPDDVIDPFESEFSSHFFSSIDHLGGPEKPRTIEETVKHEAQAMAPPKFRSSVITVGPSHCGSNQSTNIHQATTLPVSMSDRSKNVEERLDTSSGGSSGCSYGRNNKETVSGTSVTIDRKRKHVMDADQESVSQSDIGLTSTDDQTMGNKSSQRSGSTRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSRTDKASILDEAIDYLKSLQMQLQVMWMGSGMAAAAAAAASPMMFPGVQSSPYINQMAMQSQMQLSQFPVMNRSAPQNHPGLVCQNPVQLQLQAQNQILSEQLARYMGGIPQMPPAGNQMQTVQQQPADMLGFGSPAGPQSQLSAPATTDSLHMGKIG 593D72C1F2ED86C7 pif7_arath Transcription factor PIF7 BHLH72 EN109 Phytochrome-interacting factor 7 Transcription factor EN 109 bHLH transcription factor bHLH072 PIF7 Basic helix-loop-helix protein 72 MFB13.4 At5g61270 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNYGVKELTWENGQLTVHGLGDEVEPTTSNNPIWTQSLNGCETLESVVHQAALQQPSKFQLQSPNGPNHNYESKDGSCSRKRGYPQEMDRWFAVQEESHRVGHSVTASASGTNMSWASFESGRSLKTARTGDRDYFRSGSETQDTEGDEQETRGEAGRSNGRRGRAAAIHNESERRRRDRINQRMRTLQKLLPTASKADKVSILDDVIEHLKQLQAQVQFMSLRANLPQQMMIPQLPPPQSVLSIQHQQQQQQQQQQQQQQQQQFQMSLLATMARMGMGGGGNGYGGLVPPPPPPPMMVPPMGNRDCTNGSSATLSDPYSAFFAQTMNMDLYNKMAAAIYRQQSDQTTKVNIGMPSSSSNHEKRD FB76B80512E3A307 phyb_arath Phytochrome B PHYB HY3 OOP1 Protein LONG HYPOCOTYL 3 Protein OUT OF PHASE 1 MSF3.17 At2g18790 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLIETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRALRGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKFINIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGSCCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGAFCFLQIPSPELQQALAVQRRQDTECFTKAKELAYICQVIKNPLSGMRFANSLLEATDLNEDQKQLLETSVSCEKQISRIVGDMDLESIEDGSFVLKREEFFLGSVINAIVSQAMFLLRDRGLQLIRDIPEEIKSIEVFGDQIRIQQLLAEFLLSIIRYAPSQEWVEIHLSQLSKQMADGFAAIRTEFRMACPGEGLPPELVRDMFHSSRWTSPEGLGLSVCRKILKLMNGEVQYIRESERSYFLIILELPVPRKRPLSTASGSGDMMLMMPY DIP protein P14713 original sequence version: 107 DIP protein P14713 original sequence version: 131 7B5348CB1091B813 hfr1_arath Transcription factor HFR1 Transcription factor EN 68 bHLH transcription factor bHLH026 HFR1 BHLH26 EN68 Basic helix-loop-helix protein 26 Reduced sensitivity to far-red light Protein LONG HYPOCOTYL IN FAR-RED 1 FBI1 Protein REDUCED PHYTOCHROME SIGNALING REP1 RSF1 At1g02340 T6A9.4 T6A9_13 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNNQAFMELGWRNDVGSLAVKDQGMMSERARSDEDRLINGLKWGYGYFDHDQTDNYLQIVPEIHKEVENAKEDLLVVVPDEHSETDDHHHIKDFSERSDHRFYLRNKHENPKKRRIQVLSSDDESEEFTREVPSVTRKGSKRRRRDEKMSNKMRKLQQLVPNCHKTDKVSVLDKTIEYMKNLQLQLQMMSTVGVNPYFLPATLGFGMHNHMLTAMASAHGLNPANHMMPSPLIPALNWPLPPFTNISFPHSSSQSLFLTTSSPASSPQSLHGLVPYFPSFLDFSSHAMRRL BC75C5EB26E10A20 pif6_arath Transcription factor PIF6 PIF6 Transcription factor EN 111 bHLH transcription factor bHLH132 Basic helix-loop-helix protein 132 T17J13.50 At3g62090 BHLH132 EN111 PIL2 Protein PHYTOCHROME-INTERACTING FACTOR 6 Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMFLPTDYCCRLSDQEYMELVFENGQILAKGQRSNVSLHNQRTKSIMDLYEAEYNEDFMKSIIHGGGGAITNLGDTQVVPQSHVAAAHETNMLESNKHVDDSETLKASSSKRMMVDYHNRKKIKFIPPDEQSVVADRSFKLGFDTSSVGFTEDSEGSMYLSSSLDDESDDARPQVPARTRKALVKRKRNAEAYNSPERNQRNDINKKMRTLQNLLPNSHKDDNESMLDEAINYMTNLQLQVQMMTMGNRFVTPSMMMPLGPNYSQMGLAMGVGMQMGEQQFLPAHVLGAGLPGINDSADMLRFLNHPGLMPMQNSAPFIPTENCSPQSVPPSCAAFPNQIPNPNSLSNLDGATLHKKSRKTNR 15AD124E07949873 ubiq_arath Ubiquitin UBQ1 UBQ2 UBQ3 UBQ4 UBQ5 UBQ6 UBQ7 UBQ10 UBQ11 UBQ14 UBQ15 UBQ16 RPL40B RPL40A RPS27AC RPS27AB RUB2 RUB1 RPS27AA F3C22.8 F22O6_30 F9C22.10 F15A17_270 MOK16.15 T1M15.20 T17J13.210 F14M4.6 T20F21.24 C17L7.240 T32N4.13 T5J8.21 T19E23.13 F28C11.5 F26F24.28 At3g52590 At2g36170 At5g03240 At5g20620 At3g62250 At2g47110 At2g35635 At4g05320 At4g05050 At4g02890 At1g31340 At1g23410 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 852936277CD8403E pif3_arath Transcription factor PIF3 Phytochrome-associated protein 3 Basic helix-loop-helix protein 8 PIF3 PAP3 BHLH8 At1g09530 F14J9.19 Transcription factor EN 100 bHLH transcription factor bHLH008 Phytochrome-interacting factor 3 EN100 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLFELFRLTKAKLESAQDRNPSPPVDEVVELVWENGQISTQSQSSRSRNIPPPQANSSRAREIGNGSKTTMVDEIPMSVPSLMTGLSQDDDFVPWLNHHPSLDGYCSDFLRDVSSPVTVNEQESDMAVNQTAFPLFQRRKDGNESAPAASSSQYNGFQSHSLYGSDRARDLPSQQTNPDRFTQTQEPLITSNKPSLVNFSHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAKDSEDKVLNESVASATPKDNQKACLISEDSCRKDQESEKAVVCSSVGSGNSLDGPSESPSLSLKRKHSNIQDIDCHSEDVEEESGDGRKEAGPSRTGLGSKRSRSAEVHNLSERRRRDRINEKMRALQELIPNCNKVDKASMLDEAIEYLKSLQLQVQIMSMASGYYLPPAVMFPPGMGHYPAAAAAMAMGMGMPYAMGLPDLSRGGSSVNHGPQFQVSGMQQQPVAMGIPRVSGGGIFAGSSTIGNGSTRDLSGSKDQTTTNNNSNLKPIKRKQGSSDQFCGSS DIP protein O80536 original sequence version: 116 DIP protein O80536 original sequence version: 85 1044AC01D598DE7C pif1_arath Transcription factor PIF1 EN101 BHLH15 PIL5 Protein PHY-INTERACTING FACTOR 1 Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 Transcription factor EN 101 bHLH transcription factor bHLH015 Basic helix-loop-helix protein 15 T2G17.2 At2g20180 PIF1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MHHFVPDFDTDDDYVNNHNSSLNHLPRKSITTMGEDDDLMELLWQNGQVVVQNQRLHTKKPSSSPPKLLPSMDPQQQPSSDQNLFIQEDEMTSWLHYPLRDDDFCSDLLFSAAPTATATATVSQVTAARPPVSSTNESRPPVRNFMNFSRLRGDFNNGRGGESGPLLSKAVVRESTQVSPSATPSAAASESGLTRRTDGTDSSAVAGGGAYNRKGKAVAMTAPAIEITGTSSSVVSKSEIEPEKTNVDDRKRKEREATTTDETESRSEETKQARVSTTSTKRSRAAEVHNLSERKRRDRINERMKALQELIPRCNKSDKASMLDEAIEYMKSLQLQIQMMSMGCGMMPMMYPGMQQYMPHMAMGMGMNQPIPPPSFMPFPNMLAAQRPLPTQTHMAGSGPQYPVHASDPSRVFVPNQQYDPTSGQPQYPAGYTDPYQQFRGLHPTQPPQFQNQATSYPSSSRVSSSKESEDHGNHTTG DIP protein Q8GZM7 original sequence version: 95 93572A0D4669D350 pif1-pif1 2 15 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure pif1-pif3 2 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role prey prey physical association physical association no figure pif1-hfr1 2 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey physical association physical association no figure pif1-hfr1-1 4 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey physical association physical association Fig. 1 pif1-pif1-1 4 15 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1 pif1-pif3-1 4 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role prey prey physical association physical association Fig. 1 pif1-pif4 4 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association Fig. 1 pif1-pif5 4 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1 pif3-pif1 4 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1 pif5-pif1 4 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey physical association physical association Fig. 1 pif6-pif1 4 12 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association Fig. 1 bhlh72-pif1 4 9 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association Fig. 1 hfr1-pif1 4 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 1 pif1-pif3-2 1 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 15 unspecified role unspecified role bait bait region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 2A pif1-pif1-2 1 15 unspecified role unspecified role bait bait region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 15 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 2A pif3-pif3-2 1 14 unspecified role unspecified role bait bait region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 2A pif3-pif1-1 1 15 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role bait bait region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 2A pif3-pif1-2 6 14 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2B pif3-ubq1 3 14 unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6B Blue light Dark phyb-pif3-6 5 10 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig. 7A phyb-pif1-1 5 10 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig. 7A