mpidb The Microbial Protein Interaction Database Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates. Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates. J. Bacteriol. (0021-9193) 2011 imex curation Hsieh F.C., Chen C.T., Weng Y.T., Peng S.S., Chen Y.C., Huang L.Y., Hu H.T., Wu Y.L., Lin N.C., Wu W.F. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Hsieh F.C., Chen C.T., Weng Y.T., Peng S.S., Chen Y.C., Huang L.Y., Hu H.T., Wu Y.L., Lin N.C., Wu W.F. Accepted 2012-DEC-10 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates. Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates. J. Bacteriol. (0021-9193) 2011 imex curation Hsieh F.C., Chen C.T., Weng Y.T., Peng S.S., Chen Y.C., Huang L.Y., Hu H.T., Wu Y.L., Lin N.C., Wu W.F. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hsieh F.C., Chen C.T., Weng Y.T., Peng S.S., Chen Y.C., Huang L.Y., Hu H.T., Wu Y.L., Lin N.C., Wu W.F. Accepted 2012-DEC-10 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation hslu_ecoli ATP-dependent protease ATPase subunit HslU hslU htpI b3931 JW3902 Unfoldase HslU Heat shock protein HslU protein protein ecoli Escherichia coli (strain K12) MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEELRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFRKKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKIKDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQALTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGARRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL DIP protein P0A6H5 original sequence version: 98 DIP protein P0A6H5 original sequence version: 97 DIP protein P0A6H5 original sequence version: 96 D2188639EA9AEF4C sula_ecoli Cell division inhibitor SulA sulA sfiA b0958 JW0941 protein protein ecoli Escherichia coli (strain K12) MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPMMTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVESMVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSGLKIHSNLYH C76B4493773C77C2 hslu-sula-1 2 4 unspecified role unspecified role prey prey P0AFZ5:p.Met89Ile P0AFZ5-89-89 mutation mutation certain certain sequence position certain certain certain sequence position certain M I Wed May 02 19:06:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met89ile' Tue Jun 30 19:48:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met89Ile' 3 unspecified role unspecified role bait bait P0A6H5:p.Gly90Asp P0A6H5-90-90 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 15:00:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly90asp' Tue Jun 30 17:28:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly90Asp' physical association physical association figure 2, 3, 5 and 6 hslu-sula-2 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait P0A6H5:p.Gly90Asp P0A6H5-90-90 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 16:29:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly90asp' Tue Jun 30 16:02:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly90Asp' P0A6H5:p.Tyr91Phe P0A6H5-91-91 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 17:02:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr91phe' Tue Jun 30 17:52:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr91Phe' P0A6H5:p.Glu266Gln P0A6H5-266-266 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 17:13:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu266gln' Tue Jun 30 20:10:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu266Gln' P0A6H5:p.Tyr408Ala P0A6H5-408-408 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 16:20:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr408ala' Tue Jun 30 17:10:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr408Ala' p0a6h5-- P0A6H5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction figure 7