UniProt UniProt http://www.uniprot.org Characterization of the EFC/F-BAR domain protein, FCHO2. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells (1356-9597) 2011 mimix curation Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp 20-Jun-2012: Contacted by JYOTI. in vitro In vitro chromatography chromatography technology column chromatography chromatography predetermined predetermined participant predetermined Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp Accepted 2012-MAY-04 by JYOTI Genes Cells (1356-9597) 2011 mimix curation Characterization of the EFC/F-BAR domain protein, FCHO2. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells (1356-9597) 2011 mimix curation Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp 20-Jun-2012: Contacted by JYOTI. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Genes Cells (1356-9597) 2011 mimix curation Characterization of the EFC/F-BAR domain protein, FCHO2. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells (1356-9597) 2011 mimix curation Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp 20-Jun-2012: Contacted by JYOTI. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Genes Cells (1356-9597) 2011 mimix curation Characterization of the EFC/F-BAR domain protein, FCHO2. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells (1356-9597) 2011 mimix curation Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp 20-Jun-2012: Contacted by JYOTI. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H. hnakanis@gpo.kumamoto-u.ac.jp Accepted 2012-MAY-04 by JYOTI 20-Jun-2012: Contacted by JYOTI. Genes Cells (1356-9597) 2011 mimix curation a7bfv9_rat protein protein rat Rattus norvegicus (Rat) Rat MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLKKSGLPDLILGKIWDLADTDGKGVLNKQEFFIALRLVACAQNGLEVSLSSLNLAVPPPRFHDSSSPLLTSGTSVAELPWAVKSEDKAKYDAIFDSLSPVDGFLSGDKVKPVLLNSKLPVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSTLLAHIWALCDTKNCGKLSKDQFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNTIGSSPVADFSAIKELDTLNNEIIDLQREKNNVEQDLKEKEDTVRQRTTEAQDLQDEVQRESLNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAEITSQESQISTYEEELSKAREELSRLQQETAQLEESVESGKAQLEPLQQHLQDSQQEISSMQMRLAMKDLETDNNQSNWCSSPQSILVNGAADYCSLSTSSSETANLNEHAEGQNNLESEPIHPESSVRSSPEIAPSDVTDESEVVTAADIEKVSPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFATSSTDPFSASSNSSNTSVETWKHNDPFAPGGTVVAATDSATDPFASVFGNESFGDGFADFSTLSKVSNEDPFNPTISSSASSVVIPKPVLEETPSKSEDVPPALPPKTGTPTRPCPPPPGKRPINKLDSSDPFKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSSTTANKEAEPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA 8AC00E465C9DB8D7 fcho2_human F-BAR domain only protein 2 FCHO2 protein protein human Homo sapiens Human MVMAYFVENFWGEKNSGFDVLYHNMKHGQISTKELADFVRERATIEEAYSRSMTKLAKSASNYSQLGTFAPVWDVFKTSTEKLANCHLDLVRKLQELIKEVQKYGEEQVKSHKKTKEEVAGTLEAVQTIQSITQALQKSKENYNAKCVEQERLKKEGATQREIEKAAVKSKKATDTYKLYVEKYALAKADFEQKMTETAQKFQDIEETHLIHIKEIIGSLSNAIKEIHLQIGQVHEEFINNMANTTVESLIQKFAESKGTGKERPGLIEFEECDTASAVEGIKPRKRKTFALPGIIKKEKDAESVECPDADSLNIPDVDEEGYSIKPETNQNDTKENHFYSSSDSDSEDEEPKKYRIEIKPMHPNNSHHTMASLDELKVSIGNITLSPAISRHSPVQMNRNLSNEELTKSKPSAPPNEKGTSDLLAWDPLFGPSLDSSSSSSLTSSSSARPTTPLSVGTIVPPPRPASRPKLTSGKLSGINEIPRPFSPPVTSNTSPPPAAPLARAESSSSISSSASLSAANTPTVGVSRGPSPVSLGNQDTLPVAVALTESVNAYFKGADPTKCIVKITGDMTMSFPSGIIKVFTSNPTPAVLCFRVKNISRLEQILPNAQLVFSDPSQCDSNTKDFWMNMQAVTVYLKKLSEQNPAASYYNVDVLKYQVSSNGIQSTPLNLATYWKCSASTTDLRVDYKYNPEAMVAPSVLSNIQVVVPVDGGVTNMQSLPPAIWNAEQMKAFWKLSSISEKSENGGSGSLRAKFDLSEGPSKPTTLAVQFLSEGSTLSGVDFELVGTGYRLSLIKKRFATGRYLADC 0FCFBC5D814B5242 fcho2_mouse F-BAR domain only protein 2 Fcho2 protein protein mouse Mus musculus Mouse MVMAHFVENFWGEKNNGFDVLYHNMKHGQISTKELADFVRERATIEEAYSRSMTKLAKSASNYSQLGTFAPMWDVFKTSTEKLANCHLDLVRKLQELIKEVQKYGEEQVKSHKKTKEEVAGTLEAVQAIQNITQALQKSKENYTAKCVEQERLKKEGATQREIEKAAVKSKKATDTYKLYVEKYALTKADFEQKMTETAQKFQDIEETHLIHIKEIIGSLSNAVKEIHLQIGQVHEEFINNMANTTIESLIQKFAESKGTGKERPGLIEFEECDPASAVEGIKPRKRKTFALPGIIKKEKDAESVECPDADSLNIPDVDEEGFSIKPEANQNDTKENHFYSSSDSDSEDEEPKRYRIEIKPAHPNNLHHTMASLDELKVSIGNITLSPAVSRHSPVQMNRNSSNEELTKSKPSSLPTEKGTNDLLAWDPLFGSSLESSSAPLTSSSSARPTTPLSLGTLVPPPRPASRPKLASGKLSGINEIPRPFSPPVTSNTSPPPTAPLARAESSSSISSSASLSAANTPTVGVSRGPSPVSLGNQDTLPVAIALTESVNAYFKGADPTKCIVKITGDVTISFPSGIIKVFTSNPSPAVLCFRVKNISRLEQILPNSQLVFSDPSQCDSNTKDFWMNMQAVTIYLKKLSEQNPAASYYNVDVLKYQVSSNGIQSTPLNLATYWKCSASTTDLRVDYKYNPEAMVAPSVLSNIQVVVPVDGGVTNMQSLPPAIWNAEQMKAFWKLSGISEKSDSGGSGSLRAKFDLSEGPSKPTTLAVQFLSEGNTLSGVDIELVGTGYRLSLVKKRFATGRYLADC DIP protein Q3UQN2 original sequence version: 18 79FA8C2AFE6B606B eps15_human Epidermal growth factor receptor substrate 15 EPS15 AF1P Protein AF-1p protein protein human Homo sapiens Human MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA A1B9FE15B47ABAFF ap2a1_human AP-2 complex subunit alpha-1 AP2A1 ADTAA CLAPA1 Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit Alpha-adaptin A Clathrin assembly protein complex 2 alpha-A large chain 100 kDa coated vesicle protein A Adaptor-related protein complex 2 subunit alpha-1 protein protein human Homo sapiens Human MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF DIP protein O95782 original sequence version: 149 D9FB569E7EDDF6ED fcho2 1 7 unspecified role unspecified role self self direct interaction direct interaction fig 3 fcho2-eps15 3 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey direct interaction direct interaction fig 5A eps15-fcho2-2 4 8 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization fig 7A fcho2-ap2a1 2 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association fig 5B ap2a1-fcho2 4 9 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization fig 7A fcho2-eps15-1 2 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association fig 5B