I2D I2D p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. Oncogene (0950-9232) 2012 imex curation Damiano L., Le Devedec S.E., Di Stefano P., Repetto D., Lalai R., Truong H., Xiong J.L., Danen E.H., Yan K., Verbeek F.J., De Luca E., Attanasio F., Buccione R., Turco E., van de Water B., Defilippi P. Cancer - Interactions investigated in the context of cancer paola.defilippi@unito.it 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database rat-mtln3 Rat mammary adenocarcinoma mtln3 Rat mammary adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Damiano L., Le Devedec S.E., Di Stefano P., Repetto D., Lalai R., Truong H., Xiong J.L., Danen E.H., Yan K., Verbeek F.J., De Luca E., Attanasio F., Buccione R., Turco E., van de Water B., Defilippi P. Cancer - Interactions investigated in the context of cancer paola.defilippi@unito.it Accepted 2018-JUL-02 AT 14:49 BST AT 14:49 BST by ORCHARD Oncogene (0950-9232) 2012 imex curation Curated by: I2D - Interologous Interaction Database p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. Oncogene (0950-9232) 2012 imex curation Damiano L., Le Devedec S.E., Di Stefano P., Repetto D., Lalai R., Truong H., Xiong J.L., Danen E.H., Yan K., Verbeek F.J., De Luca E., Attanasio F., Buccione R., Turco E., van de Water B., Defilippi P. Cancer - Interactions investigated in the context of cancer paola.defilippi@unito.it 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Damiano L., Le Devedec S.E., Di Stefano P., Repetto D., Lalai R., Truong H., Xiong J.L., Danen E.H., Yan K., Verbeek F.J., De Luca E., Attanasio F., Buccione R., Turco E., van de Water B., Defilippi P. Cancer - Interactions investigated in the context of cancer paola.defilippi@unito.it Accepted 2018-JUL-02 AT 14:49 BST AT 14:49 BST by ORCHARD Oncogene (0950-9232) 2012 imex curation Curated by: I2D - Interologous Interaction Database p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. Oncogene (0950-9232) 2012 imex curation Damiano L., Le Devedec S.E., Di Stefano P., Repetto D., Lalai R., Truong H., Xiong J.L., Danen E.H., Yan K., Verbeek F.J., De Luca E., Attanasio F., Buccione R., Turco E., van de Water B., Defilippi P. Cancer - Interactions investigated in the context of cancer paola.defilippi@unito.it 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Damiano L., Le Devedec S.E., Di Stefano P., Repetto D., Lalai R., Truong H., Xiong J.L., Danen E.H., Yan K., Verbeek F.J., De Luca E., Attanasio F., Buccione R., Turco E., van de Water B., Defilippi P. Cancer - Interactions investigated in the context of cancer paola.defilippi@unito.it Accepted 2018-JUL-02 AT 14:49 BST AT 14:49 BST by ORCHARD Oncogene (0950-9232) 2012 imex curation Curated by: I2D - Interologous Interaction Database src8_human Src substrate cortactin CTTN EMS1 Amplaxin Oncogene EMS1 protein protein human Homo sapiens Human MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ DIP protein Q14247 original sequence version: 148 7799326C2B4383BB src8_rat Src substrate cortactin Cttn protein protein rat Rattus norvegicus (Rat) Rat MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEARRKLEEQARAKKQTPPASPSPQPAEDRPPSSPIYEDAAPLKAEPSYGSSEPEPEYSTEAAGLPEASNQQGLAYTSEPVYETTEVPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDVITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ 059CDB52681E2592 srcn1_rat SRC kinase signaling inhibitor 1 Srcin1 Snip p140Cap SNAP-25-interacting protein P140 p130Cas-associated protein protein protein rat Rattus norvegicus (Rat) Rat MQPWQCLRRFALAWWERTAEGRARSPREEVGPRDPGGRGEPDPERSSPPMLSADDAEYPREYRTLGGGGSGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPASHLASSSPPPGLPSGLPSGLPSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPTSQGVSPSPSAILERRDVKPDEDLAGKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAGDPFAYPGAGGLYKRGSVRSLSTYSAAALQSDLEDSLYKAGAGGPLYGDGYGFRLPPSSPQKLADVSAPSGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKARSTGASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKIEGSNGAATPSAPVCGSGSRSSGATPVSGPPPPAVSSTPAGQPTAVSRLQMQMHLRGLQNSASDLRGQLQQLRKLQLQNQESVRALLKRTEAELSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVAHNHRLVPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRVVTDTLAQIRRQVDEGVWPPPNNLLNQSPKKVAAETDFSKGLDFEIPPPSPPLNLHELSGPAEGTPLTPKSGNPTKGLDAPSKRNMDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKTHPGPTPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSVFIKKAESEELEIQKPQVKLRRAVSEVVRPASTPPIMASAIKDEDDEERIIAELESGGGSVPPMKVVTPGASRLKAAQGPAGSPDKGKHGKQRTEYMRIQAQQQVRVGYQAPRPLEGCTPRLCYMPLS 8C9A2E630FB31C33 srcn1_mouse SRC kinase signaling inhibitor 1 p140Cap SNAP-25-interacting protein Srcin1 Kiaa1684 P140 p130Cas-associated protein protein protein mouse Mus musculus Mouse MQPWQCLRRFALAWWERTAEGRARSPREEVGPRDPGGRGEPDPERSSPPMLSADDAEYPREYRTLGGGGGGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQVGGWTVDPVCLLSSLCSHLHGDSTPSGAGQPAQQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPASHLASSSPPPGLPSGLPSGLPSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPTGQGVSPSPSAILERRDVKPDEDLAGKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAETHSHTRARAACTSGVPCALSAPTPLPRCSPTWRTRCTRRALAALYGDGYGFRLPPSSPQKLADVSAPSGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKARSTGSASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKVEGSNGAATPSAPVCGSGSKSSGATPVSGPPPPSASSTPAGQPTAVSRLQMQLHLRGLQNSASDLRGQLQQLRNVQLQNQESVRALLKPTEADVSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVAHNHRLMPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRGVTDTLAQIRRQVDEGMWPPPNNLLNQSPKKVAAETDFSKGLDFEIPPPSPPLNLHELSGPAEGTPLTPKSTNPTKCLDASSKRNTDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKTHPGPAPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSVFIKKAESEELEVQKPQVKLRRAVSEVVRPASTPPIMASAIKDEDDEERIIAELESGGSSVPPMKVVTPGASRLKAAQGPAGSPDKGKHGKQRTEYMRIQAQQQATKPSKEVSGPNETSSPGSEKPSGSRTSIPVLTSFGARNSSISF 85F394C9523F78C0 cttn-srcin1-1 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Therefore, p140Cap was immunoprecipitated from MTLn3-EGFR GFP Ctr cells, and p140Cap and cortactin were shown to co-immunoprecipitate (Figure 6b) 6b cttn-srcin1-3 2 5 unspecified role unspecified role bait bait sh3 region required to bind necessary binding region required to bind range range range range region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association 6c cttn-srcin1-5 3 7 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey physical association physical association 6d