NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells. c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells. BMC Cancer 2011 imex curation Chen Y., Xu J., Borowicz S., Collins C., Huo D., Olopade O.I. folopade@bsd.uchicago.edu human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Chen Y., Xu J., Borowicz S., Collins C., Huo D., Olopade O.I. folopade@bsd.uchicago.edu Accepted 2012-JUN-11 by ORCHARD BMC Cancer 2011 imex curation c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells. c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells. BMC Cancer 2011 imex curation Chen Y., Xu J., Borowicz S., Collins C., Huo D., Olopade O.I. folopade@bsd.uchicago.edu human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Chen Y., Xu J., Borowicz S., Collins C., Huo D., Olopade O.I. folopade@bsd.uchicago.edu Accepted 2012-JUN-11 by ORCHARD BMC Cancer 2011 imex curation brca1_human_gene breast cancer 1, early onset gene gene human Homo sapiens Human myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA DIP protein P01106 original sequence version: 195 DIP protein P01106 original sequence version: 196 DIP protein P01106 original sequence version: 148 DIP protein P01106-1 original sequence version: 161 DIP protein P01106 original sequence version: 182 DIP protein P01106 original sequence version: 197 DIP protein P01106-1 original sequence version: 208 DIP protein P01106 original sequence version: 121 DIP protein P01106-1 original sequence version: 144 DIP protein P01106 original sequence version: 144 ED5C028029A4C5D1 myc-brca1 1 3 unspecified role unspecified role prey prey e-box x region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined e-box y region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells c-Myc 4 unspecified role unspecified role bait bait human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells association association Figure 4A Target gene BRCA1 regulatory regions assessed in the ChIP-experiments: E box X: -912 to -907 E box Y: -1292 to -1286 myc-brca1-1 2 3 unspecified role unspecified role prey prey e box y binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined e box x binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait association association Figure 4A