mpidb The Microbial Protein Interaction Database Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. J. Bacteriol. (0021-9193) 2011 imex curation Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. Accepted 2012-DEC-12 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. J. Bacteriol. (0021-9193) 2011 imex curation Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. Accepted 2012-DEC-12 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. J. Bacteriol. (0021-9193) 2011 imex curation Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. Accepted 2012-DEC-12 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. J. Bacteriol. (0021-9193) 2011 imex curation Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. Accepted 2012-DEC-12 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation ftsz_ecoli Cell division protein FtsZ ftsZ sfiB sulB b0095 JW0093 protein protein ecoli Escherichia coli (strain K12) MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNTDAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAAGMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSLITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMSEMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFETVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQPVMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD DIP protein P0A9A6 original sequence version: 83 DIP protein P0A9A6 original sequence version: 82 DIP protein P0A9A6 original sequence version: 72 B3A53340367DBBA0 zapc_ecoli Cell division protein ZapC b0946 JW5125 zapC ycbW FtsZ-associated protein C Z-ring-associated protein C protein protein ecoli Escherichia coli (strain K12) MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAFSPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHGEMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKIMNDRLKPQVNVDSFSLEQAV DIP protein P75862 original sequence version: null 7A8CC66ECE2D1B87 zapc-ftsz 3 6 unspecified role unspecified role bait bait P75862:p.Leu22Pro P75862-22-22 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Wed May 02 14:24:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu22pro' Tue Jun 30 17:34:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu22Pro' 5 unspecified role unspecified role prey prey physical association physical association Table 4 ftsz-zapc-5 1 6 unspecified role unspecified role neutral component neutral component P75862:p.Leu22Pro P75862-22-22 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:20:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu22pro' Tue Jun 30 19:51:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu22Pro' 5 unspecified role unspecified role neutral component neutral component physical association physical association figure 5 ftsz-zapc-4 3 5 unspecified role unspecified role unspecified role unspecified role 6 unspecified role unspecified role unspecified role unspecified role P75862:p.Leu22Pro P75862-22-22 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:52:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu22pro' Tue Jun 30 17:45:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu22Pro' physical association physical association figure 6 ftsz-ftsz-7 2 5 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 6 Addition of ZapC enhanced light scattering by FtsZ polymers ftsz-ftsz-8 4 5 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 7 ZapC promotes associations between FtsZ filaments.