IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. J. Biol. Chem. (0021-9258) 2011 imex curation Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. J. Biol. Chem. (0021-9258) 2011 imex curation Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay tag visualisation tag visualisation Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. The cleavage of the protein is monitored through the detection of a biotin tag that is located in the irreversible inhibitor Biotin-FPR-cmk that is added to the reaction. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. J. Biol. Chem. (0021-9258) 2011 imex curation Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu Accepted 2012-APR-30 by JYOTI J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. J. Biol. Chem. (0021-9258) 2011 imex curation Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation mca4_arath Metacaspase-4 AMC4 AMC7 MCP2D Metacaspase 2d Metacaspase-7 YUP8H12R.4 At1g79340 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKAVLIGINYPGTKAELRGCVNDVRRMYKCLVERYGFSEENITVLIDTDESSTQPTGKNIRRALADLVESADSGDVLVVHYSGHGTRLPAETGEDDDTGFDECIVPCDMNLITDDDFRDLVDKVPPGCRMTIISDSCHSGGLIDEAKEQIGESTKKEAEDEDESEESSSRFGFRKFLRSKVEGAIESRGFHIGGNKKDEDEAEEIETKEIELEDGETIHAKDKSLPLQTLIDILKQQTGNDNIEVGKIRPSLFDAFGDDSSPKVKKFMKVILGKLQAGNGEEGGLMGMLGKLASGFLEGKLNDEDYVKPAMQTHVGSKEEVYAGGSRGSVPLPDSGILISGCQTDQTSADATPAGKPTEAYGAMSNSIQTILEETDGEISNREMVTRARKALKKQGFTQQPGLYCHDGYANAPFIC F1E844F476FB51C4 amc4 3 5 putative self putative self neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O64517:p.Cys139Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A Wed May 02 17:54:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys139ala' Tue Jun 30 19:44:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys139Ala' O64517:p.Lys225Gly mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K G Wed May 02 17:11:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys225gly' Tue Jun 30 19:37:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys225Gly' This is the cleavage site of this protease. ecoli Escherichia coli (strain K12) putative self interaction putative self interaction true 2a, 2b, 2e and S2a Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-1 1 5 enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 enzyme target enzyme target substrate substrate neutral component neutral component purified purified t7 tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O64517:p.Cys139Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 15:32:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys139ala' Tue Jun 30 17:54:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys139Ala' This mutation inactivates this protease. ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 4a (right) Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-2 4 5 enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O64517:p.Cys139Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 19:25:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys139ala' Tue Jun 30 19:26:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys139Ala' This mutation inactivates this protease. t7 tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 4a (left) Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-amc4 4 5 putative self putative self neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) putative self interaction putative self interaction true 5b, 6a, 6b, S5b and S6 Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-1 2 5 putative self putative self putative self putative self purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range putative self interaction putative self interaction true 5b, 6a, 6b and S6 (right) Ca2+ is required for the amc4 protease to show this autocatalytic activity. The cleavage is inhibited by FPR-cmk and FK-tmbk.