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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>mpidb</shortLabel>
        <fullName>The Microbial Protein Interaction Database</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
      </xref>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Acyl-homoserine lactone binding to and stability of the orphan Pseudomonas aeruginosa quorum-sensing signal receptor QscR.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="21097632" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6413325" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-18377" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Acyl-homoserine lactone binding to and stability of the orphan Pseudomonas aeruginosa quorum-sensing signal receptor QscR.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2011</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Oinuma K., Greenberg E.P.</attribute>
            <attribute name="author-announcement">18-Dec-2012: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-18377" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="21097632" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>molecular sieving</shortLabel>
            <fullName>molecular sieving</fullName>
            <alias type="go synonym" typeAc="MI:0303">Gel Filtration</alias>
            <alias type="go synonym" typeAc="MI:0303">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Gel Filtration</alias>
            <alias type="synonym" typeAc="MI:1041">Sizing column</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0071" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1175" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="7708014" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Oinuma K., Greenberg E.P.</attribute>
          <attribute name="accepted">Accepted 2012-DEC-12 by ORCHARD</attribute>
          <attribute name="author-announcement">18-Dec-2012: Contacted by IntAct-Help.</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2011</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>g3xd77_pseae</shortLabel>
          <alias type="gene name" typeAc="MI:0301">qscR</alias>
          <alias type="locus name" typeAc="MI:0305">PA1898</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="G3XD77" version="TrEMBL_10" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAG05287" version="TrEMBL_63" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6452950" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAG05287" version="TrEMBL_63" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="PA1898" version="TrEMBL_63" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000976" version="TrEMBL_63"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0001216" version="TrEMBL_63"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009372" version="TrEMBL_63"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032993" version="TrEMBL_63"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1900377" version="TrEMBL_63"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_003118960.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_250589.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR016032"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005143"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000792"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036388"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036693"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="208964">
          <names>
            <shortLabel>pseae</shortLabel>
            <fullName>Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)</fullName>
          </names>
        </organism>
        <sequence>MHDEREGYLEILSRITTEEEFFSLVLEICGNYGFEFFSFGARAPFPLTAPKYHFLSNYPGEWKSRYISEDYTSIDPIVRHGLLEYTPLIWNGEDFQENRFFWEEALHHGIRHGWSIPVRGKYGLISMLSLVRSSESIAATEILEKESFLLWITSMLQATFGDLLAPRIVPESNVRLTARETEMLKWTAVGKTYGEIGLILSIDQRTVKFHIVNAMRKLNSSNKAEATMKAYAIGLLN</sequence>
        <attributeList>
          <attribute name="crc64">D4A62CD5ADDD57EE</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-18377-1">
        <names>
          <shortLabel>phzr-phzr-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-1511" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6413334" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-18377-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">figure 3</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>