mpidb The Microbial Protein Interaction Database Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B. Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B. J. Bacteriol. (0021-9193) 2010 imex curation Rowland SL, Wadsworth KD, Robson SA, Robichon C, Beckwith J, King GF 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli affinity techniques affinity technology affinity techniques tag fluorescence tag visualisation by fluorescence tag fluorescence Rowland SL, Wadsworth KD, Robson SA, Robichon C, Beckwith J, King GF Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation pbp2b_bacsu Penicillin-binding protein 2B Penicillin-binding protein B pbpB Penicillin-sensitive peptidoglycan D,D-transpeptidase BSU15160 protein protein bacsu Bacillus subtilis MPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDTATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGISFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNVYLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLAAAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGIDLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISASTAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKAGQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPKADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD B173ABEF5F1C944C divib_bacsu Cell division protein DivIB dds Cell division and sporulation protein BSU15240 divIB Division initiation protein DivIB protein protein bacsu Bacillus subtilis MNPGQDREKIVNIEERIPKIKEQRKQKANRRLISFIMLFFIMVLIIVYLQTPISKVSTISVTGNENVSKKEIIDLSDINSGDTEFWSLDKQKTEKKIQQNKLVKKAEISKSLPNKINIAIEEYKAIAYLEKDDVYYEVLENGSVLPNEVTPDDAGPILVNWTNAKKRSQMAKQLDALSNSLKQSISEIYYTPVKMDENRIKLYMNDGYVVTASIKTFADRMKTYPSIISQLSSNKKGIIHLEVATYFEEFGKNDKAAKKEDEN 7CBEC797F4352864 divib-pbpb-3 1 3 unspecified role unspecified role bait bait p16655-- P16655-- fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region P16655-123-263 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role prey prey q07868-- Q07868-- gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2