<?xml version='1.0' encoding='UTF-8'?>
<entrySet xmlns="http://psi.hupo.org/mi/mif300" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/mi/mif300 https://raw.githubusercontent.com/HUPO-PSI/miXML/master/3.0/src/MIF300.xsd" level="3" version="0" minorVersion="0">
  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>mpidb</shortLabel>
        <fullName>The Microbial Protein Interaction Database</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
      </xref>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>The flagellar basal body-associated protein FlgT is essential for a novel ring structure in the sodium-driven Vibrio motor.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="20729351" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6412270" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-18364" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">The flagellar basal body-associated protein FlgT is essential for a novel ring structure in the sodium-driven Vibrio motor.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Terashima H., Koike M., Kojima S., Homma M.</attribute>
            <attribute name="author-announcement">04-Feb-2013: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-18364" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="20729351" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="663">
            <names>
              <shortLabel>vibal</shortLabel>
              <fullName>Vibrio alginolyticus</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>pull down</shortLabel>
            <fullName>pull down</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1223" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Terashima H., Koike M., Kojima S., Homma M.</attribute>
          <attribute name="accepted">Accepted 2012-DEC-17 by ORCHARD</attribute>
          <attribute name="author-announcement">04-Feb-2013: Contacted by IntAct-Help.</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>p74944_vibal</shortLabel>
          <alias type="gene name synonym" typeAc="MI:0302">motY</alias>
          <alias type="orf name" typeAc="MI:0306">Vag1382_19510</alias>
          <alias type="gene name" typeAc="MI:0301">MotY</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P74944" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6412283" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="TrEMBL_119"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009279" version="TrEMBL_119"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR041544"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006665"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006690"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2ZF8"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036737"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="663">
          <names>
            <shortLabel>vibal</shortLabel>
            <fullName>Vibrio alginolyticus</fullName>
          </names>
        </organism>
        <sequence>MKKWLITSGVVFSLFSTSSFAVMGKRYVATPQQSQWEMVVNTPLECQLVHPIPSFGDAVFSSRANKKINLDFELKMRRPMGETRNVSLISMPPPWRPGEHADRITNLKFFKQFDGYVGGQTAWGILSELEKGRYPTFSYQDWQSRDQRIEVALSSVLFQNKYNAFSDCISNLLKYSFEDIAFTILHYERQGDQLTKASKKRLSQIADYIRHNQDIDLVLVATYTDSTDGKSASQSLSERRAESLRDYFQSLGLPEDRIQVQGYGKRRPIADNGSPIGKDKNRRVVISLGRTQV</sequence>
        <attributeList>
          <attribute name="crc64">BF543DA04222AA31</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>d9n551_vibal</shortLabel>
          <alias type="orf name" typeAc="MI:0306">Vag1382_07110</alias>
          <alias type="gene name" typeAc="MI:0301">flgT</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="D9N551" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6412288" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR032370"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR032386"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR032388"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3W1E"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR038165"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR038180"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="663">
          <names>
            <shortLabel>vibal</shortLabel>
            <fullName>Vibrio alginolyticus</fullName>
          </names>
        </organism>
        <sequence>MKKILNSLFSITLVMLVPFKVMASWYEVTGVATIVSSEETARLHALEDALFKAVNFSGADIGSISNLMPLLEESRNEYQFTNHEVRYILVESERKRRGKVEVKIRVDIYPSATGCHTDQYKKTILVGNIEVASPQQAVMGQIYQVGDDFSRVVNRQLDQTSRSFVSVGTTDYSISSNYPARTQMIAQDNGAQYIIGGVITDLTATVESQLLQDDIINRQFALEMKVFDGKTGHEVFNKAYREVARWPFAKTSQVDTRSARFWASTYGEMMLRVSRNIMLDLESELSCKITLPEVVAVFGNTVTMDLGRMHGVKEGDKLQLWHTASFIDQNGLPRNKVSQSEITLTVSRIYEHEAELTIDQPNLASSVQIGDVMNKIL</sequence>
        <attributeList>
          <attribute name="crc64">E8A0B33007CF7964</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-18364-1">
        <names>
          <shortLabel>flgt-moty</shortLabel>
        </names>
        <xref>
          <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-1502" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6412279" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-18364-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>d9n551--</shortLabel>
                  <fullName>D9N551--</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-6412294" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>his tag</shortLabel>
                    <fullName>his tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">Hexa-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">6-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Hexa-His-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">6-His-tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
          </participant>
          <participant id="7">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Figure 7</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>