mpidb The Microbial Protein Interaction Database Phosphorylation and dephosphorylation among Dif chemosensory proteins essential for exopolysaccharide regulation in Myxococcus xanthus. Phosphorylation and dephosphorylation among Dif chemosensory proteins essential for exopolysaccharide regulation in Myxococcus xanthus. J. Bacteriol. (0021-9193) 2010 imex curation Black W.P., Schubot F.D., Li Z., Yang Z. weblack@vt.edu Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Black W.P., Schubot F.D., Li Z., Yang Z. weblack@vt.edu Accepted 2013-FEB-06 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Phosphorylation and dephosphorylation among Dif chemosensory proteins essential for exopolysaccharide regulation in Myxococcus xanthus. Phosphorylation and dephosphorylation among Dif chemosensory proteins essential for exopolysaccharide regulation in Myxococcus xanthus. J. Bacteriol. (0021-9193) 2010 imex curation Black W.P., Schubot F.D., Li Z., Yang Z. weblack@vt.edu Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Black W.P., Schubot F.D., Li Z., Yang Z. weblack@vt.edu Accepted 2013-FEB-06 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation o85781_myxxa difC protein protein myxxa Myxococcus xanthus MDRTLLLCGTSSSGWRRSVTGCRSRAVREVVVPPERFTRVPRAPLAITGVMNLRGRVVTVVELRQLLSLPEGPTPPGRVVLLDRGRRDLGLLVTDVDGIEAVERVSTAPGKMTPAIRGVARLGGLGVTVLDPEGLDAAVVALFTHSK 08589FDC9C471E84 o85783_myxxa Chemotaxis protein CheA difE HK404_12450 protein protein myxxa Myxococcus xanthus MTMDMSRYLGLFISEATDHLEALGRDLVELEREGSSSAVDSMFRHAHSVKGMASSMGFEPIAIVAHRVEDLVDAVRQDRGRLDRDLVDLLLTAADTMLAQVRAVAEGKQPDDSAALLTQLSQRVTTMTGQAPAATRVAKVTALKPEGGGSDGTGGGSDGSGTAGGSGTAGGSGTTGGAGGSGTTGGGSDGSGTTGGGPDGSGTAGGAGGSSGTTGGAGGGSGTAGGAGGSGPTGAGASPASTHGGGAAGSGTRSCVDGGSGAAGGSASPGVASGTGVDGAGPGPASPNLMNGGPGTSATSSAPNGAHGTSAPLSPDGATGGVVVPLSTRRASDVPGTDAGSAAPTPDLANSLKAAASAPLPETLAQRWAVRLRIAPTCQVPGVRAFLVHKRLTNLGTLVELRPALEELKAGRIPDGNIQLELETPAGEAGIHAALKNVAEVEVLSVKPAVAAPVVQPTLVPVPDGARASSDTASRTVRVRTELLDYFLDTVGELMLATARLREVGKVLPENTRPALEEGVYRLHTLVKDLHDKVMTARMTPLSLITDRLPRAARDIARRKEREVDLVITGAEIELDRAILEELSDPLLHLLRNCIDHGLEAPEDRAAAKKGPRGRVLVAVKRARDRVIIELEDDGRGMDPAKLKNAAVSRGLLSAEAAVRLTDREAFMLSCLPGVSTAKDITDISGRGVGMDAVKRVVENVGGTLEIDSERGRGTRFTLRLPLTVAVVHLLLVEVGEEVFGLPIAKVVGATEADGESLSRSRETALLPHGNSLLPVHSLDALVGVPAPRRLGARPFVVMDGDSGRVALAVDRLLGQEEVVLKPLSRPLDLLPGLSGVTILGSGRPVFILDVPRLLSA 0CA94314DD5AF420 q7wvt8_myxxa difG HK404_12445 protein protein myxxa Myxococcus xanthus MSQPLHSDAQLDALREVANIGCGHAANALSRLMGGRKVDLSIPRVLLTGPSDAAELLGGAAPVVSGWLGIQGGLRGALLLVLPQQDGAALEALLLEGQPAIHQTERDSVMAETANIVASACLSAMGRLTGWKLVPTVPTVRRGRARDVVSDAVGQVEGDASSVVVLEARFLATAAPPVGGQLLLVLARDSIRDLLARLGV C754BD031E3C2433 o85782_myxxa difD BHS05_33420 BHS06_31730 HK404_12455 HNV27_33310 HNV28_07480 protein protein myxxa Myxococcus xanthus MAKRVLVVDDAIFMRNMIKDIFASGGFEVVGEAANGLEAVEKYKELKPDLTTMDIVMPFKSGIEATREIIKADSSAVVIMCSALGQESLVMEAIEAGASDFIVKPFRAEDVLAVVKKVLGET 4D555ED2DE21FFDF o85779_myxxa difA protein protein myxxa Myxococcus xanthus MGLALTGWMYASDGLINFVFPALSAGGKDAVRIGVAIIITGAAAALLPSWLARVTRVKVLSRSAYEISQGDLSKPVAAEGGSKRDEIDELTGAITRMQENLRELVGKIQETAKSVADTAIDLQRSAENVNGSTEEVGSSMEKIAGGAESQSQLVSKASKVITEMAGSIQRTTASAEDAARTTAETSSAAEDGSKAARLAGDKVKKVFNRIESASQQVFAFGEKTQEISKIVDAITQVAQQTNLLALNATIEAARAGEYGRGFAVVADEVRKLAESAGRSAEQISKLARDISGQSTSVVSAMKEGIAELAEGREDLTNIVRSMGAITDTIRKGSEKVHLISESAREQLKGSEEMVTAIEEIKLVARNNASSTEAIQAVIQEQTAAVSRMTSLASELTNLSVELQSVVRSFRLGP D4064E613C48221A dife-1 1 4 putative self putative self putative self putative self n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true figures 1, 2 difd-dife-5 2 4 phosphate donor phosphate donor unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 6 phosphate acceptor phosphate acceptor unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer figure 4 difd-difg-1 2 6 phosphate donor phosphate donor unspecified role unspecified role region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 phosphate acceptor phosphate acceptor unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer figure 5 difa-difc-2 1 3 inhibitor inhibitor unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 putative self putative self putative self putative self n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 inhibitor inhibitor unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association figures 1, 2