UniProt UniProt http://www.uniprot.org Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions mouse-forebrain Mus musculus, forebrain brain_forebrain electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy immunostaining immunostaining Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions mouse-forebrain Mus musculus, forebrain brain_forebrain confocal microscopy confocal microscopy immunostaining immunostaining Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI BMC Neurosci 2010 Only protein-protein interactions imex curation Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence. BMC Neurosci 2010 imex curation Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin J., Kittanakom S., Wong V., Reyes B.A., Van Bockstaele E.J., Stagljar I., Berrettini W., Levenson R. Origin of OPRM1 overexpressed from 293-MOR cells (gift from v. Zastrow) accoording pmid 1371121. Experiments involving D2IC2 and CAPS (positive control, fig 1) not included as species origin of the proteins cannot be established. rlevenson@hmc.psu.edu Accepted 2012-APR-30 by JYOTI 20-Jun-2012: Contacted by JYOTI. BMC Neurosci 2010 Only protein-protein interactions imex curation wls_human Protein wntless homolog WLS C1orf139 GPR177 Integral membrane protein GPR177 Protein evenness interrupted homolog Putative NF-kappa-B-activating protein 373 UNQ85/PRO18667 protein protein human Homo sapiens Human MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE 0DAFDF51BBA0C288 oprm_human Mu-type opioid receptor OPRM1 MOR1 Mu opioid receptor Mu opiate receptor protein protein human Homo sapiens Human MDSSAAPTNASNCTDALAYSSCSPAPSPGSWVNLSHLDGNLSDPCGPNRTDLGGRDSLCPPTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQLENLEAETAPLP 1DABEBEC9AFF6F66 wls_rat Protein wntless homolog Wls Gpr177 Integral membrane protein GPR177 Protein evenness interrupted homolog protein protein rat Rattus norvegicus (Rat) Rat MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVSYVAAKCVDVRKNHHKTRWLMPWGPNKCNKINDFEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQIDIAFKLNNQIRENAEVSMDVSLGYRDDMFSEWTEMAHERVPRKLRCTFTSPKTPEHEGRHYECDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE 58DCC18634B86668 oprm_rat Mu-type opioid receptor Oprm1 Ror-b Opioid receptor B protein protein rat Rattus norvegicus (Rat) Rat MDSSTGPGNTSDCSDPLAQASCSPAPGSWLNLSHVDGNQSDPCGLNRTGLGGNDSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLP 9C916DE7C1C33743 wls_mouse Protein wntless homolog Wls Gpr177 Integral membrane protein GPR177 Protein evenness interrupted homolog protein protein mouse Mus musculus Mouse MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVPYTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE DIP protein Q6DID7 original sequence version: 52 14BDD97D6050D976 oprm_mouse Mu-type opioid receptor Oprm1 Mor Oprm protein protein mouse Mus musculus Mouse MDSSAGPGNISDCSDPLAPASCSPAPGSWLNLSHVDGNQSDPCGPNRTGLGGSHSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGNILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSARIRQNTREHPSTANTVDRTNHQLENLEAETAPLP DIP protein P42866 original sequence version: 69 DIP protein P42866 original sequence version: 70 DFE1C758E2DA197E oprm1-wls 9 12 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure oprm1-wls-1 5 11 unspecified role unspecified role prey prey c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 25 physical association physical association 1 oprm1-wls-2 1 11 unspecified role unspecified role prey prey region s tag s tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 31 29 physical association physical association 2A oprm1-wls-3 2 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2B oprm1-wls-4 3 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2C oprm1-wls-5 4 14 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 3A oprm1-wls-6 10 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association 3A oprm1-wls-7 8 15 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component colocalization colocalization 4C oprm1-wls-8 6 15 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component colocalization colocalization 4C oprm1-wls-9 7 11 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed colocalization colocalization 5A Morphine treatment causes an increased colocalization at the plasm,a membrane. DAMGO treatment causes an increased colocalization within cytosolic compartments. oprm1-wls-10 2 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5B morphine DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-enkephalin)