mpidb The Microbial Protein Interaction Database Crystal structure of activated CheY1 from Helicobacter pylori. Crystal structure of activated CheY1 from Helicobacter pylori. J. Bacteriol. (0021-9193) 2010 imex curation Lam K.H., Ling T.K., Au S.W. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lam K.H., Ling T.K., Au S.W. Accepted 2013-FEB-18 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Crystal structure of activated CheY1 from Helicobacter pylori. Crystal structure of activated CheY1 from Helicobacter pylori. J. Bacteriol. (0021-9193) 2010 imex curation Lam K.H., Ling T.K., Au S.W. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lam K.H., Ling T.K., Au S.W. Accepted 2013-FEB-18 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation o25675_helpy Flagellar motor switch protein FliM HP_1031 protein protein helpy Helicobacter pylori Campylobacter pylori MADILSQEEIDALLEVVDENVDIQNVQKKDIIPQRSVTLYDFKRPNRVSKEQLRSFRSIHDKMARNLSSQVSSIMRSIVEIQLHSVDQMTYGEFLMSLPSPTSFNVFSMKPMGGTGVLEINPSIAFPMIDRLLGGKGSAYDQNREFSDIELNLLDTILRQVMQILKEVWSPVVEMFPTIDAKESSANVVQIVAQNEISIMVVLEIIIGHSRGMMNICYPVISIESILSKMGSRDLMLSETNSKKSRNKELQALLSGVSVDMIVFLGAVELSLKEMLDLDVGDTIRLNKIANDEVSVYVHKKKRYLASVGFQGYRKTIQIKEVIYSEKERTKEILEMLEEQRRGKVGDIMKIEEE AE815F7E3FD21926 cheay_helpy Sensor histidine kinase CheAY cheAY HP_0392 protein protein helpy Helicobacter pylori Campylobacter pylori MDDLQEIMEDFLIEAFEMNEQLDQDLVELEHNPEDLDLLNRIFRVAHTIKGSSSFLNLNILTHLTHNMEDVLNRARKGEIKITPDIMDVVLRSIDLMKTLLVTIRDTGSDTNNGKENEIEEAVKQLQAITSQNLESAKERTTEAPQKENKEETKEEAKEENKENKAKAPTAENTSSDNPLADEPDLDYANMSAEEVEAEIERLLNKRQEADKERRAQKKQEAKPKQEVTPTKETPKAPKTETKAKAKADTEENKAPSIGVEQTVRVDVRRLDHLMNLIGELVLGKNRLIRIYSDVEERYDGEKFLEELNQVVSSISAVTTDLQLAVMKTRMQPVGKVFNKFPRMVRDLSRELGKSIELIIEGEETELDKSIVEEIGDPLIHIIRNSCDHGIEPLEERRKLNKPETGKVQLSAYNEGNHIVIKISDDGKGLDPVMLKEKAIEKGVISERDAEGMSDREAFNLIFKPGFSTAKVVSNVSGRGVGMDVVKTNIEKLNGIIEIDSEVGVGTTQKLKIPLTLAIIQALLVGVQEEYYAIPLSSVLETVRISQDEIYTVDGKSVLRLRDEVLSLVRLSDIFKVDAILESNSDVYVVIIGLADQKIGVIVDYLIGQEEVVIKSLGYYLKNTRGIAGATVRGDGKITLIVDVGAMMDMAKSIKVNITTLMNESENTKSKNSPSDYIVLAIDDSSTDRAIIRKCLKPLGITLLEATNGLEGLEMLKNGDKIPDAILVDIEMPKMDGYTFASEVRKYNKFKNLPLIAVTSRVTKTDRMRGVESGMTEYITKPYSGEYLTTVVKRSIKLEGDQS B6D9B0FC82B54E27 chey1_helpy Chemotaxis protein CheY1 cheY1 HP_1067 protein protein helpy Helicobacter pylori Campylobacter pylori MKLLVVDDSSTMRRIIKNTLSRLGYEDVLEAEHGVEAWEKLDANADTKVLITDWNMPEMNGLDLVKKVRSDSRFKEIPIIMITTEGGKAEVITALKAGVNNYIVKPFTPQVLKEKLEVVLGTND F2B40BC0C04FCBFA o25153-chey 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait o25153-- O25153-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined o25153-- O25153-- phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 7A flim-chey-1 2 3 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction Fig. 2C chey-o25153-2 1 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal O25153-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7B