mpidb The Microbial Protein Interaction Database The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. J. Bacteriol. (0021-9193) 2010 imex curation Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. Only protein-protein interactions in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. Accepted 2013-FEB-17 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. J. Bacteriol. (0021-9193) 2010 imex curation Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. Only protein-protein interactions ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. Accepted 2013-FEB-17 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. J. Bacteriol. (0021-9193) 2010 imex curation Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. Only protein-protein interactions ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. Accepted 2013-FEB-17 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation rpoa_ecoli DNA-directed RNA polymerase subunit alpha rpoA pez phs sez b3295 Transcriptase subunit alpha RNA polymerase subunit alpha JW3257 protein protein ecoli Escherichia coli (strain K12) MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE DIP protein P0A7Z4 original sequence version: 55 DIP protein P0A7Z4 original sequence version: 60 DIP protein P0A7Z4 original sequence version: 69 DIP protein P0A7Z4 original sequence version: 107 DIP protein P0A7Z4 original sequence version: 93 12A14B75A3CAEA19 rl2_ecoli 50S ribosomal protein L2 rplB Large ribosomal subunit protein uL2 JW3279 b3317 protein protein ecoli Escherichia coli (strain K12) MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK DIP protein P60422 original sequence version: 81 1010BDE8D8C68B9B rpoa-rplb 3 5 unspecified role unspecified role prey prey p60422-- P60422-- myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait p0a7z4-- P0A7Z4-- t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3 rpoa-rpoa-1 2 4 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Table 3 rpoa-rplb-1 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Table 3 rpoa-rplb-2 1 5 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association Figs 1, 2