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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>mpidb</shortLabel>
        <fullName>The Microbial Protein Interaction Database</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
      </xref>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Functional analysis of the stability determinant AlfB of pBET131, a miniplasmid derivative of bacillus subtilis (natto) plasmid pLS32.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="20023009" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6409889" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-18323" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Functional analysis of the stability determinant AlfB of pBET131, a miniplasmid derivative of bacillus subtilis (natto) plasmid pLS32.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Tanaka T.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">teruot@scc.u-tokai.ac.jp</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-18323" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="20023009" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="4932">
            <names>
              <shortLabel>yeasx</shortLabel>
              <fullName>Saccharomyces cerevisiae (Baker's yeast)</fullName>
              <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>2 hybrid</shortLabel>
            <fullName>two hybrid</fullName>
            <alias type="go synonym" typeAc="MI:0303">2H</alias>
            <alias type="go synonym" typeAc="MI:0303">2h</alias>
            <alias type="go synonym" typeAc="MI:0303">Gal4 transcription regeneration</alias>
            <alias type="go synonym" typeAc="MI:0303">two-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">yeast two hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">2-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">classical two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">classical two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">2H</alias>
            <alias type="synonym" typeAc="MI:1041">two-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">2-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Gal4 transcription regeneration</alias>
            <alias type="synonym" typeAc="MI:1041">2h</alias>
            <alias type="synonym" typeAc="MI:1041">yeast two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Y2H</alias>
            <alias type="synonym" typeAc="MI:1041">Y-2H</alias>
            <alias type="synonym" typeAc="MI:1041">2 hybrid</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0018" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-94" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="10967325" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="12634794" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="1946372" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Tanaka T.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">teruot@scc.u-tokai.ac.jp</attribute>
          <attribute name="accepted">Accepted 2013-FEB-16 by ORCHARD</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>e9rjg4_bacna</shortLabel>
          <alias type="gene name" typeAc="MI:0301">alfA</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="E9RJG4" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6409907" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR040607"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_013603336.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="YP_004243646.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR043129"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="86029">
          <names>
            <shortLabel>bacillus subtilis su</shortLabel>
            <fullName>Bacillus subtilis subsp. natto</fullName>
          </names>
        </organism>
        <sequence>MTLTTVIDIGNFSTKYAYKDKKQIKVGSFPSILHSYKPLEDYEGMERVEYNGLDYYVGETVKNFYFGREEQMYFGNTRKGHMEGQIRLVYALYTIFKETGKKEFNLILTCPYESMVTDKKYFVQHFEGEREVIVEGKSFKFTVHNIVMAAEGLGALNFSDSLNCVIVDAGSKTLNVLYLINGSISKMDSHTINGGTIDNSIMDLAKTFAKTCSNIDYDYPIVCTGGKAEEMKECLENVGYSTVSSAELGEDKPSYYVNSVGLLLKYGRKFEEMFA</sequence>
        <attributeList>
          <attribute name="crc64">8E57CE0E2246620B</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>e9rjg5_bacna</shortLabel>
          <alias type="gene name" typeAc="MI:0301">alfB</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="E9RJG5" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6409898" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003677" version="TrEMBL_19"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_009968681.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="YP_004243647.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="86029">
          <names>
            <shortLabel>bacillus subtilis su</shortLabel>
            <fullName>Bacillus subtilis subsp. natto</fullName>
          </names>
        </organism>
        <sequence>MSREDNFMYQINWNKKKYPEICKALEDAKNRTGGIAWYLRELIQKDLEEKRGGVVRSTPVYETVDQEVQNDPPKPSKAKIETIELPDDSGGFL</sequence>
        <attributeList>
          <attribute name="crc64">58218ABC42839A51</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-18323-1">
        <names>
          <shortLabel>alfa-alfb</shortLabel>
        </names>
        <xref>
          <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-1321" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6409903" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-18323-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
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                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="6">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
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                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
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                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
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            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig. 8A</attribute>
        </attributeList>
      </interaction>
      <interaction id="7" imexId="IM-18323-2">
        <names>
          <shortLabel>alfb-alfb</shortLabel>
        </names>
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        <participantList>
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                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                  <shortLabel>prey</shortLabel>
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          </participant>
          <participant id="9">
            <interactorRef>3</interactorRef>
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              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
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                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                  <shortLabel>bait</shortLabel>
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            <fullName>physical association</fullName>
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          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig. 8B</attribute>
        </attributeList>
      </interaction>
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          <shortLabel>alfa-alfa</shortLabel>
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                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
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          </participant>
          <participant id="12">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
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                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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            <experimentalRoleList>
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                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
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            <fullName>physical association</fullName>
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        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig. 8B</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
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