UniProt UniProt http://www.uniprot.org A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. Nat. Cell Biol. (1465-7392) 2010 mimix curation Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp 20-Jun-2012: Contacted by JYOTI. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Accepted 2012-MAY-30 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 mimix curation A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. Nat. Cell Biol. (1465-7392) 2010 mimix curation Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp 20-Jun-2012: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Accepted 2012-MAY-30 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 mimix curation A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. Nat. Cell Biol. (1465-7392) 2010 mimix curation Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp 20-Jun-2012: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Accepted 2012-MAY-30 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Human HeLa cell cDNA Nat. Cell Biol. (1465-7392) 2010 mimix curation A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. Nat. Cell Biol. (1465-7392) 2010 mimix curation Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp 20-Jun-2012: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Accepted 2012-MAY-30 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 mimix curation A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. Nat. Cell Biol. (1465-7392) 2010 mimix curation Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp 20-Jun-2012: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Accepted 2012-MAY-30 by ORCHARD 20-Jun-2012: Contacted by JYOTI. Human brain cDNA libraries. Nat. Cell Biol. (1465-7392) 2010 mimix curation tnip1_human TNFAIP3-interacting protein 1 TNIP1 KIAA0113 NAF1 Nef-associated factor 1 HIV-1 Nef-interacting protein Virion-associated nuclear shuttling protein Nip40-1 A20-binding inhibitor of NF-kappa-B activation 1 protein protein human Homo sapiens Human MEGRGPYRIYDPGGSVPSGEASAAFERLVKENSRLKEKMQGIKMLGELLEESQMEATRLRQKAEELVKDNELLPPPSPSLGSFDPLAELTGKDSNVTASPTAPACPSDKPAPVQKPPSSGTSSEFEVVTPEEQNSPESSSHANAMALGPLPREDGNLMLHLQRLETTLSVCAEEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTSILQTLCEQLRKENEALKAKLDKGLEQRDQAAERLREENLELKKLLMSNGNKEGASGRPGSPKMEGTGKKAVAGQQQASVTAGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ D81B96BEAD50D871 nemo_human NF-kappa-B essential modulator IKBKG FIP3 NEMO NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 FIP-3 protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein Q9Y6K9 original sequence version: 85 DIP protein Q9Y6K9 original sequence version: 118 DIP protein Q9Y6K9 original sequence version: 123 DIP protein Q9Y6K9 original sequence version: 141 DIP protein Q9Y6K9 original sequence version: 125 DIP protein Q9Y6K9 original sequence version: 156 DIP protein Q9Y6K9 original sequence version: 83 DIP protein Q9Y6K9 original sequence version: 132 DIP protein Q9Y6K9 original sequence version: 124 DIP protein Q9Y6K9 original sequence version: 90 322D1037881447FF ipa9_shifl E3 ubiquitin-protein ligase ipaH9.8 ipaH9.8 Invasion plasmid antigen ipaH9.8 RING-type E3 ubiquitin transferase ipaH9.8 SFLP090 pWR501_0234 CP0226 protein protein shifl Shigella flexneri MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSHALPALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS DIP protein Q8VSC3 original sequence version: 34 5E70042D797B5099 ikbkg-ipah9_8 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 2a and S3a and b ikbkg-ipah9_8-1 2 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 2a and S5b ipah9_8-ikbkg 2 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 2a tnip1-ipah9_8 1 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 4a, S3c and d tnip1-ipah9_8-1 2 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 4a ikbkg-ipah9_8-2 4 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association 5a ikbkg-ipah9_8-3 1 8 unspecified role unspecified role prey prey 6 ancillary ancillary prey prey 7 unspecified role unspecified role bait bait region modlys modified L-lysine residue ModLys undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NEMO was ubiquitinated (cells treated with iE-DAP to induce Nod1-stimulated ubiquitination of GST-NEMO) association association 5d ipah9_8-ikbkg-1 3 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association no figure ipah9_8-tnip1 3 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association no figure ipah9_8-tnip1-1 5 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association no figure ipah9_8-ikbkg-2 5 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association no figure ikbkg-ipah9_8-4 4 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 5a