mpidb The Microbial Protein Interaction Database AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. Mol. Microbiol. (0950-382X) 2009 imex curation Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. Mol. Microbiol. (0950-382X) 2009 imex curation Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. Mol. Microbiol. (0950-382X) 2009 imex curation Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions myxxd Myxococcus xanthus (strain DK 1622) fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation aglz_myxxd Adventurous-gliding motility protein Z MXAN_2991 aglZ protein protein myxxd Myxococcus xanthus (strain DK 1622) MERRVLIVESEHDFALSMATVLKGAGYQTALAETAADAQRELEKRRPDLVVLRAELKDQSGFVLCGNIKKGKWGQNLKVLLLSSESGVDGLAQHRQTPQAADGYLAIPFEMGELAALSHGIVPPGTDDTGASLDAALNGTREAPPPMPPSLKAAAGGPPKLPKRERRSAMTEEDRAFLDRTFQSIADRKAELLAESRQLKRPPPRRELMGTPEGKIQILRDELKTREAQLARLSEIWNVRERELLSGEDRIHEKDVELQGLKMQVDDLLRRFNEAQQATIQKEREHGATVDDLLLQKFSAEKDLIEVVASKEKDINLLRREVSRAEEELSRRAGELEHGRNEYDKLEKHLGVVTLEFEVKEQKLQDTVLANEGEIARLTKRGDDFEAELNRTISERDQRFAELDGEIQALQERLQQTEQERDTTVRGLEARAARAEEHGTQADAEIHRLNAERDALEAKLSQQVADLEADLARTMGERDQLRLDKDAQEAELTQRIEERDAKLGTLERELSETIARNEHTEAELNANIQQQLERIGELEGEVEAVKTHLEDRENELTAELQALGQAKDELETDLNDRLQALSQAKDALEADLSRQLEELRSAKAELEADLTGQIQALTSQLEETQRQLDDSQRTGEQLSARVAQLEDTVSQRESTIESLQGDVAARDQRISELSGDLEATSQTLAQTQQTLAQTEQQLADTQNTLASTEGALAETRGELDATSQTLQQTQQTLAQTEGALAETRGELDATSQTLAQTQQTLAQTEQQLADTQNTLASTEGTLAETRGELEATSQTLQQTHAALEDTRGALQETSDTLAHTTRERDQRIAELADLGAAKDALEQELTGQIGHLRSELSETQGNYEAERAAHEKLAAESSAHIGDLTSERDGLRSELEATSQTLEQTHGQLAATRDALAREQHAHQESRKAAASTQTTLEGQLAEARAHGEDLGEHLTLTKHELGTRVAELTQLTATLAQTENTRAHLEERLHTLTEESQRREELLQNDLTQKGTELSDTLRKLTHVTQEKMRQAEVLNREVATRTEQLKAMEAKLQTQATEARRQAEGLGQQITGLNEQLEQGRKALAGREDQLRAAGAAQQKLTAERDGLAGQLQQAEARLQQQAQQANQERADAKRAADELAAKLAKTEQRITQFAQDAQTQATEADARAKDLQGQLSARAKKIQDLELAVENAQGAKSRAEKELNAKVAAAESKAHEASTRLAAAQKERKDLEARHAKEQEDLAAKQKAELERRDAIKAQEVARLQQSVQEKSKALKVAELELARYKSKSATTATPAKAAAKPAAAEDDELAVRTQLNQVIAPAAAAQAPAPAKKPAAKPAAQAPAKKAPAPAPAPPAALSDESEPTDRTLVIQLPTAKEDDDWTALVDELDK 5E20D7DC3181E36A q1d118_myxxd MXAN_5512 protein protein myxxd Myxococcus xanthus (strain DK 1622) MRCYTAPDRVRRLSRHRDTPRSTKTMARTTQEQTQERHAFLLELFRQQPDISSKEALESFKDKFGGTINMKTFNQLREQAEDEAASAAAAAPAPAPEVEPEPEPEPEPEETEATVDDAASRLKAAAAPEAINGAAGAPAKKAKAKGNGPKNIFVDAPKEHLTFLEGIIQQFQEAGAANIRIDHATDRWMVVVVDSK 0B86FEC65EF94976 q1d7g6_myxxd MXAN_3206 protein protein myxxd Myxococcus xanthus (strain DK 1622) MVMKQNPLSFHPRAKPAAIAAMAAGEQGKYWEYHAKLFANQKKLDDVSLEQYAKELGLNLDKWKAELGNPKFQDIITRDQALAGQLGASGTPAFFINGRFLSGAQPIANFQALIDEELVKAENLVKGGVPASQVYAKIIEKGSERAAPKAQPQQPAATVRKVEIPSDSPSFGPANAKVTIVEWSDFECPFCSRVGPTLSKIKESYAKDVRVVFRHQPLPFHPNAKLAAEASHAAHEQGKFWEYHDKLFANQKAMDRASLEKYAQELGLNVAKFKAALDSGKFKAKVEADMAAGNAVGANGTPTFFINGREFVGAQPFEAFKRVIDEEIGKADKLLAAGTKPEELYAKLNAENVANAPTAPAAAPGAPAEPPVQKVDVGNAPVKGDKNAPVTIVAFSDFECPFCSRVVPTLKQLEDQYGGKIKVAFKNQPLPFHANAKLAAAAALAANEQGKFWEYHDKLFANQRALDRASLEKYAQELGLNVDKFKAALDQGKFNAQIEADMAQASSVGASGTPTFFINGRTLVGAQPVDAFKRVIDEELKKLGGAVADSK 8FC08D99C3A378BF q1d8s0_myxxd MXAN_2736 protein protein myxxd Myxococcus xanthus (strain DK 1622) MAQKKAKRANRDLEKTYPRKDFVAKLRRLADAIEEGKAFNIQVAGERLRIPADALFNIEHERGDGVDEVEFQLRWQRE 8719573665DB7AAC q1d2u5_myxxd MXAN_4870 protein protein myxxd Myxococcus xanthus (strain DK 1622) MEIPEDSRTLAYQASNWGRRCPSPRKTMRRSLLVCLVLLATASAAQEKKAPRDADLGRKSATAVDKSLAGDITREKKKEEVAPALQYDQFRLGVELQVASKRREQIQSLKKIISLSPDPKEAPSLLFRLGEFYWEESKFFFFEANRKDDDLIQAMNRNDAAGQQRAKAEKAEFSAKQKEYGKLAVEQYTKIVQEYPNFERTDEVLFFLGQYLMEDGQDRKALVAFKRLVEKHPQSKFIPDAYFAFGEYYFNNSKGKRPELEKALVAYKKAAEFPESQVYAFALYKQGWCHYNMGDFESAKDKFKTVVLYGELAGANALEKDGGKGGGRSSLLREARTDYVRAFAHHGDVAQARAEFGKVATNPEDRFTMLKQLANLYYGDGKDREAAITYNGLIKEKPLSPEAPGFQGRIVDCILRMGNKERTVVQVRRLVKIMKEVEGSGVIKEDKDKKLLAEAKELSERTLSNLAVTWHNEGKKTRNEETFRYADAVYSDYLTLFPENPKAYDLRFFWAELLNDSLQNFDKAAANYTLVVLQDAKVLEAKDDKGKPKPGKPGKWLQNAAYNAVLAYDEVVKAGEARGEGKSEAVGSDITKKATIPTLKKALLDACERYLKYVPKGEKRVEIAFKAANIYYRHNHFDEAVLRFSEIALGYPEYKFENGERAAEISANLILDSYHLLQDYAKVNEWARRFYANDKLAVGKFRDDLAKLIEQSSFKLVSQLEEKKEFEKAAEAYLAFVKDFPQTEIADLALYNASVDYYKAKRLDKAIEVRKRLFAEYPRSKHVPDSIYANAEALEAIGDFEDAAATYEAYVRGYERSLGDKGNAKARGGGKKKRGAVDDNKPAVVQKWDESKAQVALFNAATYREGLGQVKAALRNREHYLTLWPRAKDADEIRLSIIDLTAKSGAAFKAIKMLEEYERDNMRSPSRFLAAEGRIVDLYKKMGKTRDVARMQKRIFEHFDQLPRRVQGSLEKPALATAAQAQFLSVDLDWNEYRRLKLYWGAPPSPDRFKASIQDKSRALQVVEKKYVQTVTLGAPEPAICALHRIGLAYDHFADRLINAPMPRGLDEESEQALRDEFANQAQPLKDKATEAFSATVAKSRELDVYNDCAAESLKLLRTTYQPDRYPDMPDEKVALKGRVQLIGGDLLANIQDVPPPAPKAADAQQARAETLQEDLTDLTAQLRSQTETQVDARPAATTNGATPAKQGGNDEEPEDFL 0640C05B764C6924 q1d4v8_myxxd Histidine kinase MXAN_4140 frzE protein protein myxxd Myxococcus xanthus (strain DK 1622) MDTEALKKSLLKKFQEVTADRLQKIQLGVLDLEKETADQAAEDVARELHTMKGEARMLGLAAIGQLAHAAEDVLRAEREGKTATEVATDVLLRACDVLSDLNEDLSGANTGNPASEEMVRMLAEVSGQTPPAIAGARPVAPPPAPPPAPVAAPVVTPAAVAAPPAPVQAPVAPPPTQAPVAEPGAHAAAAAPHPAAAHGRDEEAPSAAKSAVADRSIRVNVEVLDALGLLAGDLLVESARGRLRSSETEALFERFSRLGDRFLRLAEEIDISNEVREQLDRVESDLHMLRDDAFRFVRRNDDGINTLHGNLAKMADHVAEARLVPLSTVFDAFPRAVREMSRTQGKEVDLVIENADIGVDRSMLGDVRDALVHLLRNSVDHGVESPDTRQQLGKPLNGRIRIRVRVDGDMLHIEVEDDGRGIDPERLRQAAISKRLINAVQAAALSEREAIELIFRPGFSTRDQVSELSGRGVGMDVVKRKVETLGGSVGVSSRIGRGSTITLRLPQSLALMKVLLVRLGDDVYGMPAADVEAVMRVKPDDRLEIFGTLAVRHRGKPTALVALGPLLGLNGGNRFDKPPAVVVRHGEDHAALVVDGFVDEREVAVKPCGGEFLKAAPFIAGTAALEDGRIAVLLHVPDIMAEVRRMARPVTQAPAAKRLRVLLVDDSPIARATEGALVKALGHSVEEAQDGEEAYVKVQNNTYDLILTDVQMPKLDGFSLARRLKSTPAVARIPVIILSSLASPEDKRRGLDAGADAYLVKGELGVEVLAQAIDRLT 9912BD40991C69E5 q1d4v7_myxxd MXAN_4141 frzCD protein protein myxxd Myxococcus xanthus (strain DK 1622) MSLDTPNEKPAGKARARKAPASKAGATNAASTSSSTKAITDTLLTVLSGNLQARVPKELVGESGVELAHLLNQVLDQFAASEHRKHVAAQEIDQALDALIGLVREGDLSRWNTTTEDPQLGPLLEGFGKVIETLRTFVREINEAALRLSSSANQVLAASTQHETSSTEQAAAIHETTATMEELKHASAQIAENAGSVARVAEETLGAARAGRGAIGEFIQAMQQIRSDGVAVADSIAKLSKRVERIGTVVEVIDEIADRSDLLALNAALEGSRAGEAGKGFSIVAAEMRRLAENVLDSTKEIKNLITEIREATAAAAGAAEASKSATESGEKLGAVAAQAVEGILAGVQETSDAARVINLATQQQRTATEQVVASMAEIEDVTRQTTQASKQATGAAAELTQLAGRLAELIKRFKAD 2AC3A70BB26070C7 q1d4v6_myxxd MXAN_4142 frzB protein protein myxxd Myxococcus xanthus (strain DK 1622) MDLLFFDIGATLYGTDASQVLRIDRALPEDLTLAELGLPHRGNRAIVFDTPEGEAHLKVDAVHGVRSIPVNSLRRMPPTAGAAAYAVGVCLEEARTVLLIDLVETARTQGRH 1C2407F577FA3EE1 q1d4v5_myxxd MXAN_4143 frzA protein protein myxxd Myxococcus xanthus (strain DK 1622) MAPDRALAAQARPEQEFFCFRVGDLRLGVPSENVLEVLRAGLLTPLPRTPSFIMGVTGHRGEVLPVLDLLRFLSKGEARIGPRTRLFVGVTGSYVAGVVADTVLGLRRIPVADILPPPLGGDAAAEHLLGVVQASGNQEAINLLNFSKLLQTARQRAVAR 26ED29B05B545A64 aglz-frzcd 1 4 unspecified role unspecified role neutral component neutral component region Q1D823-2-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component region Q1D4V7-2-135 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 18 15 direct interaction direct interaction Fig. 2 aglz-frzcd-1 3 4 unspecified role unspecified role neutral component neutral component region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined q1d4v7-2-280 Q1D4V7-2-280 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig. 7 frzcd-aglz-1 2 4 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 5 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 8 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 7 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 9 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myxxd Myxococcus xanthus (strain DK 1622) 6 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 12 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 11 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) association association Table 1 frzcd-aglz-2 2 4 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 6 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 8 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 7 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myxxd Myxococcus xanthus (strain DK 1622) 5 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) association association Table 1