mpidb The Microbial Protein Interaction Database ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway. ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway. Mol. Microbiol. (0950-382X) 2009 imex curation Schmidt R., Zahn R., Bukau B., Mogk A. Only protein-protein interactions in vitro In vitro protease assay protease assay tag fluorescence tag visualisation by fluorescence tag fluorescence Schmidt R., Zahn R., Bukau B., Mogk A. Accepted 2013-FEB-08 by ORCHARD Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway. ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway. Mol. Microbiol. (0950-382X) 2009 imex curation Schmidt R., Zahn R., Bukau B., Mogk A. Only protein-protein interactions ecoli Escherichia coli (strain K12) pull down pull down ms participant Identification by mass spectrometry ms participant Schmidt R., Zahn R., Bukau B., Mogk A. Accepted 2013-FEB-08 by ORCHARD Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation apha_ecoli Class B acid phosphatase aphA napA b4055 yjbP JW4015 protein protein ecoli Escherichia coli (strain K12) MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQAPIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFWEKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPATNMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTYKPLPQAGAFGEEVIVNSEY DIP protein P0AE22 original sequence version: null 35C1BFE6DF9E530D ubie_ecoli Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE ubiE b3833 yigO JW5581 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase Demethylmenaquinone methyltransferase protein protein ecoli Escherichia coli (strain K12) MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMSFGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLKMGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVLKPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQDTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF B1EEE209BDC68F5E B1EEE209BDC68F5E lipa_ecoli Lipoyl synthase Lipoic acid synthase Lipoate synthase Sulfur insertion protein LipA Lip-syn lipA lip b0628 JW0623 protein protein ecoli Escherichia coli (strain K12) MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK BE7BF32A4E3AA358 BE7BF32A4E3AA358 nhar_ecoli Transcriptional activator protein NhaR Na(+)/H(+) antiporter regulatory protein nhaR antO b0020 JW0019 protein protein ecoli Escherichia coli (strain K12) MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADALSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGLFSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYSALFSPAVR E81FAFC4FC4FD2F1 pat_ecoli Putrescine aminotransferase Putrescine--2-oxoglutaric acid transaminase JW5510 b3073 patA ygjG Diamine transaminase Cadaverine transaminase Putrescine transaminase Putrescine:2-OG aminotransferase protein protein ecoli Escherichia coli (strain K12) MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA 0B4D42A24627335D cfa_ecoli Cyclopropane-fatty-acyl-phospholipid synthase cfa cdfA b1661 JW1653 protein protein ecoli Escherichia coli (strain K12) MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR 861FF3314FDAF2C4 861FF3314FDAF2C4 clpa_ecoli ATP-dependent Clp protease ATP-binding subunit ClpA clpA lopD b0882 JW0866 protein protein ecoli Escherichia coli (strain K12) MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREALEACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNEVTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSEEQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGVGKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKNELTYGFQSAQKHKAEAAH DIP protein P0ABH9 original sequence version: 77 DIP protein P0ABH9 original sequence version: 40 DIP protein P0ABH9 original sequence version: 38 0C96A2EB64A9F7DC clps_ecoli ATP-dependent Clp protease adapter protein ClpS yljA clpS b0881 JW0865 protein protein ecoli Escherichia coli (strain K12) MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVIDVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCTLEKA DIP protein P0A8Q6 original sequence version: 43 2E9DC8000EA3CE86 dps_ecoli DNA protection during starvation protein dps pexB vtm b0812 JW0797 protein protein ecoli Escherichia coli (strain K12) MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLSLITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKTPLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLWFIESNIE DIP protein P0ABT2 original sequence version: 50 DIP protein P0ABT2 original sequence version: 93 596A79B1C6A5E60B yeah_ecoli UPF0229 protein YeaH yeaH b1784 JW1773 protein protein ecoli Escherichia coli (strain K12) MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVTDVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQGQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGVPANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAELRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILLYLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNPAQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHLQSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKG B78AFC54CCC230B3 hemy_ecoli Protein HemY hemY b3802 JW3774 protein protein ecoli Escherichia coli (strain K12) MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGLAIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQVEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRVRLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHVGDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIECDDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTLGQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTLQNNPPQ DIP protein P0ACB7 original sequence version: 39 56BDDC4E19099D3C mrec_ecoli Cell shape-determining protein MreC mreC Cell shape protein MreC Rod shape-determining protein MreC JW3219 b3250 protein protein ecoli Escherichia coli (strain K12) MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDTAVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENARLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQVVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGDVLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNGANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATAPAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ DIP protein P16926 original sequence version: null 1BAA7C5EF9E84C92 clpp_ecoli ATP-dependent Clp protease proteolytic subunit clpP lopP Endopeptidase Clp Caseinolytic protease Protease Ti Heat shock protein F21.5 b0437 JW0427 protein protein ecoli Escherichia coli (strain K12) MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN DIP protein P0A6G7 original sequence version: 51 DIP protein P0A6G7 original sequence version: 96 A7843D036C8CB3C2 argp_ecoli HTH-type transcriptional regulator ArgP argP Inhibitor of chromosome initiation OriC replication inhibitor iciA JW2883 b2916 protein protein ecoli Escherichia coli (strain K12) MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLGALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPESRMMRKVTDALLDYGHKVLRQD This sequence has been withdrawn from Uniprot. 8AD60B7B6661E3EF 8AD60B7B6661E3EF clps-apha 2 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Fig. 4, Supp Table 1 clpa-clpp 1 11 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 ancillary ancillary neutral component neutral component 15 enzyme enzyme unspecified role unspecified role 10 ancillary ancillary unspecified role unspecified role cleavage cleavage reaction cleavage Fig. 6 clps-apha-1 2 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0A8Q6:p.Asp35_Asp36delinsAlaAla mutation mutation certain certain sequence position certain certain certain sequence position certain DD AA Thu Jul 07 12:17:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp35_36ala Fri Jul 22 10:54:13 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp35ala,asp36ala' Wed May 02 16:28:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_asp35-36ala_ala' Tue Jun 30 19:56:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp35_Asp36delinsAlaAla' 7 unspecified role unspecified role prey prey association association Fig. 4, Supp Table 1