mpidb The Microbial Protein Interaction Database The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. J. Bacteriol. (0021-9193) 2009 imex curation Steen J.A., Bannam T.L., Teng W.L., Devenish R.J., Rood J.I. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag perox activity tag visualisation by peroxidase activity tag perox activity Steen J.A., Bannam T.L., Teng W.L., Devenish R.J., Rood J.I. Accepted 2013-FEB-08 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. J. Bacteriol. (0021-9193) 2009 imex curation Steen J.A., Bannam T.L., Teng W.L., Devenish R.J., Rood J.I. Only protein-protein interactions ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Steen J.A., Bannam T.L., Teng W.L., Devenish R.J., Rood J.I. Accepted 2013-FEB-08 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. J. Bacteriol. (0021-9193) 2009 imex curation Steen J.A., Bannam T.L., Teng W.L., Devenish R.J., Rood J.I. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Steen J.A., Bannam T.L., Teng W.L., Devenish R.J., Rood J.I. Accepted 2013-FEB-08 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation q1plh8_clopf pCW3_0032 tcpC G6Z03_15175 protein protein clostridium perfring Clostridium perfringens MFRKNKENTKPKKEKKEKKSFFKKKDKLNMDLEDSVANLDKQVNGVKEKKLVSVMPFRIARAFIWVLIGILCFRGGVSVFKGNQSEALKKENEKILNNMEKQSSLETQAFSFAEEFAWDYFSRYPSDTQDFVRRITKYTTEQLANEMNNGTYSDVIYTSAFYFEKYSENQVNVSVKARVRVYTPKAGQEQTPQDQLQYDTNLVDYYLEVPIVFDKDMNMAVDALPVMTAPPEKAYFKNKEFSGTSENDADKTKKITDSVSQFFKAYYEQNQTQIDYFLVDGADIKGAGQKFSFNKIDRINIYKLSDKEFLAIVDLNVDSFGNAIKQGFNLTVVQEGDKFLVKTLEPRTSNIDLNNKSNN F6C54B4178DEC58E q1plh4_clopf pCW3_0036 tcpG G6Z03_15155 protein protein clostridium perfring Clostridium perfringens MKKLFIWSGIISLIFPLLFFFLIIGGSGEQPTPVNPNPNLTEEQLNFISQIVSGAKQSYEETGIFPSITLAQAILESGWGRSGLAIKANNLFGIKADSGWKGKVLEMPTQEHVNGGIITIIARWRVYESWNESVIDHGKFFVENSRYKENGVLDAKNYVEQAQCIQKAGYATDPNYANQLIQVINDFGLNLYDMNGDVVGNDVIEKAIEAGMKWVGKSPYVWGGGRNQADVDAGRFDCSSLVHYCYASAGIQLGPRESVTTWSLINMGKPVPASEMKRGDLIFFDTAGRNGHIGIYLGNGKFLNDSSTQGVSIGDLNSAYWSRYFNGNVRRVVE 722B4871D6EC58D8 q1plh3_clopf ERS852446_03029 pCW3_0037 tcpH pTet_030 G6Z03_15150 protein protein clostridium perfring Clostridium perfringens MENVKINIYSKFLYLKNKLTYKNILKAIVIIATIVIAYNCLTKIAYADGFLSYGNSDDPYKNIPEGWGMFISEKYRNNYALDIIDTHGWELIDKAITGLTNIVFSGIVMISWFGVNIFRFCFTNDIAAGFANQLNSVLGSLNNGIFNNFFMIVFMISLFSIVYNLYKKNFSAIGSQILAVAIIYATTAFLSNGAVGFLSKTTEFSKTIGTSAIVAINGKDNNEGSLEDYSKTVVGTLWGNFVQQPWVMLEFDGKVPLNDENMTTKNSINEEALNYSKDILSEPYGSDEREKALESINEKFNKELFSKNGINTKLVSVCVLFVITLIKMIILIAVGMIQIGFQLMSILLILLLPFIMLIAIVPFFGGTNIIKGIARKYLGTQLGIIVSSFVLALLVLVDTVTLTMFQAWGANLIIALLMQCTCWIMVIAFRKQLMNGLINLQNRIAPGTAGAFRFGAKPLDKGADIAKEYAVNPAMDRAYDIGDNLITKAQYAKEYTKGNLSLYKDRKVAQAIDFLGSKYDNLKNNKPEDIENANNPKENIDGVKDTKNKVNSNENINKKPVEKFDLNGVKATKPSNITSENIPNKNINGENKPNIDEDAVTKNDIKDPIENKDIAPKTIDENIKNNGNDNGTIQDKSPGSKENKTISEDEEITTKPEEIKTPEKTINKENLNELKNENTSADKKNNDSNSSNISVTEIPEDLVEEKSVEAINKAKNDVKANKEKINNNINSKNVESNPEVKENIEVASSPEVTEIKKKSTKKVENIKNNGQPKEKSADKKEIKEVSTKERVERVKSFMGVSNKDIKSKEKHLENKLEYIKKGNSKNFKKKGS DF893D02E2890C19 q1pli0_clopf pCW3_0030 tcpA protein protein clostridium perfring Clostridium perfringens MIFLSSDWEKKVNQEIENGANAIINEIANVLRIFFFRVGLGFKKSWKNKKLFIGFFLSFLIPIAARIKSDYFLVDTKFYFKIIYFLTFIAPLFYMVIVSFVKNKEDKRNAEYRLAFEQLNFVGADSKTPILKSFIEDKGTRIDEITFESMIPIETWKSYIPQLQTSLNISIISIEQGASKRIVIIKSMAGDAKIPKYLPWDDKYIEEQEGVVVVGQTFSGNIKIDLNKSPHILSAGETGSGKSVILRCILWQLLKQGAIAYMVDFKGGVEFGLEYEKVGQVITEVDAAEKLFKYLVDENAKRLKLLRESGSKNIGEYNKKFEGEELKRIIVVIDELAELMDKTGVDDETRAKLVRIEGYTSTLARLSRATGINLCIGVQRPDAKVITGQIKNNVPVRICGRFADSKASEIVLSNTKAKDLPEVKGRFLFKLGADTVQFQAFYFDDDKHFIPNKILKLRKDEIEDKKVGLDLNKEKLIDNDLMNSVNEKFEIKEVKSMDSNIENEDDSKFENFEEQNKTENLKSTINDDYE 48E9BB270550D0DA tcpg-tcpg-2 3 5 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 5 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction Fig. 2 tcpc-tcpc-1 3 4 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 4 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction Fig. 2 tcpa-tcph-1 2 7 unspecified role unspecified role bait bait Q1PLI0:p.Asp334_Glu335delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DE AA Thu Jul 07 12:17:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp334_glu335ala Fri Jul 22 11:03:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp334ala,glu335ala' Wed May 02 18:08:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_glu334-335ala_ala' Tue Jun 30 17:07:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp334_Glu335delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association Fig. 1, 4E tcpa-tcpg 2 7 unspecified role unspecified role bait bait Q1PLI0:p.Asp334_Glu335delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DE AA Thu Jul 07 12:17:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp334_glu335ala Fri Jul 22 10:49:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp334ala,glu335ala' Wed May 02 15:44:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_glu334-335ala_ala' Tue Jun 30 15:44:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp334_Glu335delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q1PLI0:p.Gln379Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 19:02:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln379ala' Tue Jun 30 16:43:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln379Ala' 5 unspecified role unspecified role prey prey physical association physical association Fig. 1, 4D tcpa-tcpc 2 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q1PLI0:p.Asp334_Glu335delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DE AA Thu Jul 07 12:17:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp334_glu335ala Fri Jul 22 10:50:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp334ala,glu335ala' Wed May 02 17:49:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_glu334-335ala_ala' Tue Jun 30 19:49:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp334_Glu335delinsAlaAla' physical association physical association Fig. 1, 4C tcpa-tcpa 2 7 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait Q1PLI0:p.Asp334_Glu335delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DE AA Thu Jul 07 12:17:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp334_glu335ala Fri Jul 22 11:09:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp334ala,glu335ala' Wed May 02 19:48:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_glu334-335ala_ala' Tue Jun 30 15:48:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp334_Glu335delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1, 4B tcpg-tcpg-1 2 5 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 1B tcpa-tcpa-2 3 7 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 7 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction Fig. 2 tcpc-tcpc-2 2 4 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 4 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association Fig. 2 tcpa-tcph-2 1 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 6 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association Fig. 3