mbinfo MBInfo http://www.mechanobio.info/ Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. Interaction detection method: GST pull down. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-bone-marrow Mouse bone marrow bone_marrow bone marrow anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The coimmunoprecipitation was done in the presence of chemotactic stimulants such as fMLP or MIP-2. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-bone-marrow Mouse bone marrow bone_marrow bone marrow fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro in gel kinase assay in-gel kinase assay in gel kinase assay autoradiography autoradiography Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The kinase assay was carried out in fMLP-stimulated mouse bone marrow neutrophils. The autophosphorylation by Hck was quantified by densitometry. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-hl_60 Homo sapiens acute myeloid leukemia cell line hl_60 human acute myeloid leukemia cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. Interaction detection method: Protein A Sepharose pull down. Lysates were precipitated with anti-GFP antibody coupled to Protein A Sepharose and was incubated with His-HCK. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochem. Cell Biol. (0829-8211) 2009 imex curation Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Dong B., Miliotis H., Liu J., Alberts A.S., Zhang J., Siminovitch K.A. The in vitro kinase assay involving enolase (Fig 5C) could not be curated since the organism source of enolase could not be identified. Interaction detection method: Protein A Sepharose pull down. Lysates were immunoprecipitated with GFP antibodies coupled to Protein A Sepharose and the coimmunoprecipitated Flag-Hck was detected with anti-Flag antibodies. The source of Hck inferred from previous work of contributing scientist [PMID:10644735]. The source of WASP inferred from the amino acid length (502 aa). ksimin@mshri.on.ca Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Cell Biol. (0829-8211) 2009 Only protein-protein interactions imex curation diap1_mouse Protein diaphanous homolog 1 Diaphanous-related formin-1 p140mDIA Diaph1 Diap1 protein protein mouse Mus musculus Mouse MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS DIP protein O08808 original sequence version: 62 DIP protein O08808 original sequence version: 141 09404164873CA7C1 diap1_human Protein diaphanous homolog 1 DIAPH1 DIAP1 Diaphanous-related formin-1 protein protein human Homo sapiens Human MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS 9C8273DE4748564F hck_mouse Tyrosine-protein kinase HCK Hck B-cell/myeloid kinase Hematopoietic cell kinase Hemopoietic cell kinase p56-HCK/p59-HCK protein protein mouse Mus musculus Mouse MGGRSSCEDPGCPRSEGRAPRMGCVKSRFLRDGSKASKTEPSANQKGPVYVPDPTSSSKLGPNNSNSMPPGFVEGSEDTIVVALYDYEAIHREDLSFQKGDQMVVLEEAGEWWKARSLATKKEGYIPSNYVARVNSLETEEWFFKGISRKDAERHLLAPGNMLGSFMIRDSETTKGSYSLSVRDFDPQHGDTVKHYKIRTLDSGGFYISPRSTFSSLQELVLHYKKGKDGLCQKLSVPCVSPKPQKPWEKDAWEIPRESLQMEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANLMKSLQHDKLVKLHAVVSQEPIFIVTEFMAKGSLLDFLKSEEGSKQPLPKLIDFSAQISEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARIIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALEHGYRMPRPDNCPEELYNIMIRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP DF72FD69B38C9706 wasp_mouse Wiskott-Aldrich syndrome protein homolog Was Wasp protein protein mouse Mus musculus Mouse MNSGPGPVGGRPGGRGGPAVQQNIPSNLLQDHENQRLFELLGRKCWTLATTVVQLYLALPPGAEHWTMEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYLTPTPFFHTFAGDDCQVGLNFADESEAQAFRALVQEKIQKRNQRQSGERRQLPPPPAPINEERRGGLPPVPPHPGGDHGGPSGGPLSLGLVTVDIQNPDITSSRYRGLPAPGPGPTDKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPCRGGGGGGGGGGGGGGGGGGQPLRPPVVGSNKGRSGPLPPVPMGGAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPPPPPLPGAGGPPAPPPPPPPPPPPPCPGSGPAPPPLPPTPVSGGSPAPGGGRGALLDQIRQGIQLNKTPGALENSVQQPPAQQSEGLVGALMHVMQKRSRVIHSSDEGEDQTGEDEEDDEWDD DIP protein P70315 original sequence version: 114 DIP protein P70315 original sequence version: 125 9C223733C59F0C8A wasp_human Wiskott-Aldrich syndrome protein WAS IMD2 protein protein human Homo sapiens Human MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD DIP protein P42768 original sequence version: 183 DIP protein P42768 original sequence version: 168 DIP protein P42768 original sequence version: 132 DIP protein P42768 original sequence version: 155 7228428672B7CB78 hck_human Tyrosine-protein kinase HCK HCK Hemopoietic cell kinase p59-HCK/p60-HCK Hematopoietic cell kinase p59Hck p61Hck protein protein human Homo sapiens Human MGGRSSCEDPGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP DIP protein P08631 original sequence version: 120 DIP protein P08631 original sequence version: 150 DIP protein P08631-1 original sequence version: 163 847E877A0A641725 hck-diaph1-1 5 15 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association Fig 1B. The interaction of Hck with Wasp increased after chemoattractant (fMLP/MIP-2) stimulation. mDia1 association with Hck was noticed only in the presence of chemoattractant stimulation. diaph1-hck 3 12 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1C. Colocalization was seen in mice cells stimulated with the chemoattractant MIP-2. was-hck 6 14 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 15 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig 2A. His-Hck was identified to be expressed in sf9 cells by tracing it to previous work of the contributing author [PMID:10644735]. hck-was-5 7 14 unspecified role unspecified role prey prey region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 15 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig 2B. His-Hck was identified to be expressed in sf9 cells based on the previous work of the Hck cDNA contributing author [PMID:10644735]. was-hck-1 9 15 unspecified role unspecified role prey prey c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range sh3 domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3A. was-hck-2 1 14 unspecified role unspecified role bait bait proline-rich region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig 3C. His-Hck was identified to be expressed in sf9 cells based on the previous work of the Hck cDNA contributing author [PMID:10644735]. was-hck-3 2 13 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Fig 4B. The amount of Hck coimmunoprecipitated with Wasp decreased in mDia1-deificient cells. was-hck-4 8 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5A. The phopshorylation of Wasp by Hck was detected by blotting the WASP immunoprecipitates with anti-phosphotyrosine antibodies. hck-1 4 12 putative self putative self putative self putative self mouse-bone-marrow Mouse bone marrow bone_marrow bone marrow phosphorylation phosphorylation reaction phosphorylation true Fig 5C. The autophosphorylation by Hck was decreased in mDia1-deficient cells. hck-was-6 3 12 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1C, 4C. The Hck-Wasp colocalization was disrupted in mDia1-deficient cells (Fig 4C). The colocalization is also disrupted in the absence of stimulation with the chemoattractant, MIP-2. MIP-2 was-hck-5 9 14 unspecified role unspecified role bait bait proline-rich region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 3B.