IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy. Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy. J. Biol. Chem. (0021-9258) 2009 imex curation Ahuja N., Korkin D., Chaba R., Cezairliyan B.O., Sauer R.T., Kim K.K., Gross C.A. They were unable to perform similar experiments with the N-terminal domain of RseB because that domain alone forms high molecular weight (MW) aggregates. The Rse-A and the RseB constructs that were used for the pull-down were previously purified cgross@cgl.ucsf.edu 15-Nov-2012: Contacted by pporras. Only protein-protein interactions ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Ahuja N., Korkin D., Chaba R., Cezairliyan B.O., Sauer R.T., Kim K.K., Gross C.A. They were unable to perform similar experiments with the N-terminal domain of RseB because that domain alone forms high molecular weight (MW) aggregates. The Rse-A and the RseB constructs that were used for the pull-down were previously purified cgross@cgl.ucsf.edu Accepted 2012-OCT-26 by ORCHARD 15-Nov-2012: Contacted by pporras. J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy. Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy. J. Biol. Chem. (0021-9258) 2009 imex curation Ahuja N., Korkin D., Chaba R., Cezairliyan B.O., Sauer R.T., Kim K.K., Gross C.A. They were unable to perform similar experiments with the N-terminal domain of RseB because that domain alone forms high molecular weight (MW) aggregates. The Rse-A and the RseB constructs that were used for the pull-down were previously purified cgross@cgl.ucsf.edu 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ahuja N., Korkin D., Chaba R., Cezairliyan B.O., Sauer R.T., Kim K.K., Gross C.A. They were unable to perform similar experiments with the N-terminal domain of RseB because that domain alone forms high molecular weight (MW) aggregates. The Rse-A and the RseB constructs that were used for the pull-down were previously purified cgross@cgl.ucsf.edu Accepted 2012-OCT-26 by ORCHARD 15-Nov-2012: Contacted by pporras. J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation rseb_ecoli Sigma-E factor regulatory protein RseB rseB b2571 JW2555 protein protein ecoli Escherichia coli (strain K12) MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYELSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTLNGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWMDTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKAKFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQMLRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ DIP protein P0AFX9 original sequence version: 63 3F8C34DD85600B54 rsea_ecoli Anti-sigma-E factor RseA yfiJ rseA mclA b2572 JW2556 Regulator of SigE Sigma-E anti-sigma factor RseA Sigma-E factor negative regulatory protein protein protein ecoli Escherichia coli (strain K12) MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMRGDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLTQMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANNGQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ DIP protein P0AFX7 original sequence version: 71 DIP protein P0AFX7 original sequence version: 40 DIP protein P0AFX7 original sequence version: 39 DIP protein P0AFX7 original sequence version: 76 63BD12131C611C32 rsea-rseb 2 4 unspecified role unspecified role bait bait nucl transformation nucleic acid transformation nucl transformation histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey nucl transformation nucleic acid transformation nucl transformation region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFX9:p.Gln182Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:27:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln182ala' Tue Jun 30 16:52:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln182Ala' P0AFX9:p.[Asp245_Asn246delinsAlaAla;Ile249_Glu250delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DN AA certain certain sequence position certain certain certain sequence position certain IE AA Thu Jul 07 12:17:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp 245 ala, asn 246 ala, ile 249 ala, glu 250 ala Fri Jul 22 11:15:07 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp245ala,asn246ala,ile249ala,glu250ala' Wed May 02 19:14:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_asn245-246ala_ala,ile_glu249-250ala_ala' Tue Jun 30 16:17:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp245_Asn246delinsAlaAla;Ile249_Glu250delinsAlaAla]' P0AFX9:p.Ile249_Glu250delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain IE AA Thu Jul 07 12:18:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ile 249 ala, glu 250 ala Fri Jul 22 10:59:06 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ile249ala,glu250ala' Wed May 02 18:20:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile_glu249-250ala_ala' Tue Jun 30 19:19:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile249_Glu250delinsAlaAla' P0AFX9:p.Asp176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:53:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp176ala' Tue Jun 30 20:02:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp176Ala' P0AFX9:p.Ile249Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A Wed May 02 16:58:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile249ala' Tue Jun 30 20:08:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile249Ala' P0AFX9:p.Asp171Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:07:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp171ala' Tue Jun 30 18:04:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp171Ala' P0AFX9:p.Arg238_Arg239delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain RR AA Thu Jul 07 12:17:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg 238 ala, arg 239 ala Fri Jul 22 10:54:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg238ala,arg239ala' Wed May 02 15:48:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg238-239ala_ala' Tue Jun 30 20:09:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg238_Arg239delinsAlaAla' flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0AFX9:p.Glu250Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:26:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu250ala' Tue Jun 30 17:09:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Ala' P0AFX9:p.Glu178Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:49:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu178ala' Tue Jun 30 18:37:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu178Ala' ecoli Escherichia coli (strain K12) 8 10 direct interaction direct interaction Figure 2 A ,C, 5A, 6A When N-terminally Flag-tagged C-terminal domain of RseB (residues 218 –318) was allowed to interact with N-terminally His tagged RseA-peri (121-216), only about 15% of the C-terminal domain of RseB (Fig.2B, C) was bound to RseA-peri. rsea-rseb-1 2 3 unspecified role unspecified role prey prey nucl transformation nucleic acid transformation nucl transformation flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait nucl transformation nucleic acid transformation nucl transformation P0AFX7:p.Leu177Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:46:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu177ala' Tue Jun 30 17:05:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu177Ala' P0AFX7:p.Asp179Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:22:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp179ala' Tue Jun 30 17:32:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp179Ala' P0AFX7:p.Gln183Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 14:35:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln183ala' Tue Jun 30 19:04:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln183Ala' P0AFX7:p.Arg185Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:28:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg185ala' Tue Jun 30 17:13:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg185Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFX7:p.Asn174Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 20:22:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn174ala' Tue Jun 30 17:16:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn174Ala' P0AFX7:p.Glu181Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:04:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu181ala' Tue Jun 30 20:20:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu181Ala' P0AFX7:p.Leu182Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 19:34:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu182ala' Tue Jun 30 16:41:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu182Ala' trx tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0AFX7:p.Met176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A Wed May 02 19:17:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met176ala' Tue Jun 30 20:10:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met176Ala' P0AFX7:p.Tyr180Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:23:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr180ala' Tue Jun 30 19:42:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr180Ala' P0AFX7:p.Leu186Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:16:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu186ala' Tue Jun 30 18:08:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu186Ala' P0AFX7:p.Ser188Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:58:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser188ala' Tue Jun 30 16:58:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser188Ala' histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0AFX7:p.Gln178Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 14:36:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln178ala' Tue Jun 30 16:49:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln178Ala' P0AFX7:p.His187Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A Wed May 02 18:26:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187ala' Tue Jun 30 19:10:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Ala' ecoli Escherichia coli (strain K12) 24 30 direct interaction direct interaction Figure 4, 7 rsea-rseb-3 2 3 unspecified role unspecified role prey prey P0AFX9:p.Arg238_Arg239delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain RR AA Thu Jul 07 12:15:40 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg 238 ala, arg 239 ala Fri Jul 22 11:00:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg238ala,arg239ala' Wed May 02 18:17:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg238-239ala_ala' Tue Jun 30 18:32:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg238_Arg239delinsAlaAla' P0AFX9:p.[Asp245_Asn246delinsAlaAla;Ile249_Glu250delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DN AA certain certain sequence position certain certain certain sequence position certain IE AA Thu Jul 07 12:15:41 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp 245 ala, asn 246 ala, ile 249 ala, glu 250 ala Fri Jul 22 10:49:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp245ala,asn246ala,ile249ala,glu250ala' Wed May 02 15:34:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_asn245-246ala_ala,ile_glu249-250ala_ala' Tue Jun 30 16:10:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp245_Asn246delinsAlaAla;Ile249_Glu250delinsAlaAla]' P0AFX9:p.Ile249_Glu250delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain IE AA Thu Jul 07 12:18:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ile 249 ala, glu 250 ala Fri Jul 22 10:49:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ile249ala,glu250ala' Wed May 02 18:10:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile_glu249-250ala_ala' Tue Jun 30 17:05:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile249_Glu250delinsAlaAla' P0AFX9:p.Glu181Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:23:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu181ala' Tue Jun 30 16:26:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu181Ala' P0AFX9:p.Ile249Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A Wed May 02 17:46:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile249ala' Tue Jun 30 19:28:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile249Ala' P0AFX9:p.Glu250Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:44:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu250ala' Tue Jun 30 15:56:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Ala' P0AFX9:p.Asp174Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:51:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp174ala' Tue Jun 30 17:02:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp174Ala' P0AFX9:p.Asp176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:49:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp176ala' Tue Jun 30 17:48:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp176Ala' P0AFX9:p.Glu178Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:55:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu178ala' Tue Jun 30 18:14:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu178Ala' P0AFX9:p.Gln182Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 17:11:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln182ala' Tue Jun 30 17:25:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln182Ala' flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFX9:p.Asp171Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:49:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp171ala' Tue Jun 30 18:55:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp171Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 59 55 direct interaction direct interaction Figures 5B, 6B rsea-rseb-2 2 4 unspecified role unspecified role bait bait nucl transformation nucleic acid transformation nucl transformation P0AFX7:p.Leu186Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:22:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu186ala' Tue Jun 30 19:19:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu186Ala' histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trx tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0AFX7:p.Tyr180Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 18:23:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr180ala' Tue Jun 30 19:49:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr180Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFX7:p.Leu182Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:49:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu182ala' Tue Jun 30 17:39:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu182Ala' P0AFX7:p.Ser188Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:17:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser188ala' Tue Jun 30 17:01:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser188Ala' P0AFX7:p.Asn174Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 14:41:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn174ala' Tue Jun 30 16:44:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn174Ala' P0AFX7:p.Met176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A Wed May 02 16:08:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met176ala' Tue Jun 30 18:11:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met176Ala' P0AFX7:p.His187Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A Wed May 02 15:34:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187ala' Tue Jun 30 18:23:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Ala' P0AFX7:p.Arg185Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:13:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg185ala' Tue Jun 30 16:29:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg185Ala' P0AFX7:p.Gln178Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 18:09:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln178ala' Tue Jun 30 19:38:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln178Ala' P0AFX7:p.Glu189Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:56:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu189ala' Tue Jun 30 17:01:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu189Ala' P0AFX7:p.Glu181Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:54:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu181ala' Tue Jun 30 18:37:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu181Ala' P0AFX7:p.Gln183Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:51:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln183ala' Tue Jun 30 19:21:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln183Ala' P0AFX7:p.Ile173Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A Wed May 02 18:49:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile173ala' Tue Jun 30 16:02:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile173Ala' P0AFX7:p.Leu177Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:44:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu177ala' Tue Jun 30 17:52:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu177Ala' P0AFX7:p.Asp179Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:16:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp179ala' Tue Jun 30 17:47:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp179Ala' ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey nucl transformation nucleic acid transformation nucl transformation P0AFX9:p.Ile249_Glu250delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain IE AA Thu Jul 07 12:15:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ile 249 ala, glu 250 ala Fri Jul 22 11:08:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ile249ala,glu250ala' Wed May 02 15:58:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile_glu249-250ala_ala' Tue Jun 30 18:10:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile249_Glu250delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 82 66 direct interaction direct interaction Figure 7 rsea-rseb-4 1 3 unspecified role unspecified role prey prey nucl transformation nucleic acid transformation nucl transformation region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0AFX9:p.Pro248Cys mutation mutation certain certain sequence position certain certain certain sequence position certain P C Wed May 02 14:57:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro248cys' Tue Jun 30 18:29:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro248Cys' 4 unspecified role unspecified role bait bait nucl transformation nucleic acid transformation nucl transformation histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0AFX7:p.Leu186Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L C Wed May 02 16:28:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu186cys' Tue Jun 30 16:26:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu186Cys' P0AFX7:p.Asp179Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain D C Wed May 02 18:58:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp179cys' Tue Jun 30 19:58:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp179Cys' P0AFX7:p.Ile173Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain I C Wed May 02 16:30:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile173cys' Tue Jun 30 18:34:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile173Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 94 86 physical association physical association Figure 8 rsea-rseb-5 1 4 unspecified role unspecified role bait bait nucl transformation nucleic acid transformation nucl transformation P0AFX7:p.His187Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain H C Wed May 02 18:03:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187cys' Tue Jun 30 16:14:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Cys' P0AFX7:p.Asp179Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain D C Wed May 02 14:48:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp179cys' Tue Jun 30 17:04:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp179Cys' histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey nucl transformation nucleic acid transformation nucl transformation flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0AFX9:p.Ser251Cys mutation mutation certain certain sequence position certain certain certain sequence position certain S C Wed May 02 15:36:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser251cys' Tue Jun 30 18:49:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser251Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 100 104 physical association physical association Figure 8