mpidb The Microbial Protein Interaction Database Glucose- and glucokinase-controlled mal gene expression in Escherichia coli. Glucose- and glucokinase-controlled mal gene expression in Escherichia coli. J. Bacteriol. (0021-9193) 2009 imex curation Lengsfeld C., Schönert S., Dippel R., Boos W. Binding of glucokinase was demonstrated in an inverted membrane vesicles exposing the EIIB domain of PtsG were produced from a pstG deletion strain. Glucokinase was recovered by ultracentrifugation in the washed pellet fraction. Only protein-protein interactions ecoli Escherichia coli (strain K12) affinity techniques affinity technology affinity techniques anti tag western anti tag western blot anti tag western Lengsfeld C., Schönert S., Dippel R., Boos W. Binding of glucokinase was demonstrated in an inverted membrane vesicles exposing the EIIB domain of PtsG were produced from a pstG deletion strain. Glucokinase was recovered by ultracentrifugation in the washed pellet fraction. Accepted 2013-FEB-04 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation ptgcb_ecoli PTS system glucose-specific EIICB component ptsG glcA umg b1101 EIICB-Glc JW1087 protein protein ecoli Escherichia coli (strain K12) MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH D97A80FD64B74F73 D97A80FD64B74F73 glk_ecoli Glucokinase glk b2388 JW2385 Glucose kinase protein protein ecoli Escherichia coli (strain K12) MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFTAVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGHVDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDITERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL 2D6CD956641A3823 glk-ptsg-1 1 3 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 2 unspecified role unspecified role neutral component neutral component iib region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association Fig. 2