mpidb The Microbial Protein Interaction Database Spa32 interaction with the inner-membrane Spa40 component of the type III secretion system of Shigella flexneri is required for the control of the needle length by a molecular tape measure mechanism. Spa32 interaction with the inner-membrane Spa40 component of the type III secretion system of Shigella flexneri is required for the control of the needle length by a molecular tape measure mechanism. Mol. Microbiol. (0950-382X) 2008 imex curation Botteaux A., Sani M., Kayath C.A., Boekema E.J., Allaoui A. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Botteaux A., Sani M., Kayath C.A., Boekema E.J., Allaoui A. Accepted 2013-FEB-01 by ORCHARD Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation Spa32 interaction with the inner-membrane Spa40 component of the type III secretion system of Shigella flexneri is required for the control of the needle length by a molecular tape measure mechanism. Spa32 interaction with the inner-membrane Spa40 component of the type III secretion system of Shigella flexneri is required for the control of the needle length by a molecular tape measure mechanism. Mol. Microbiol. (0950-382X) 2008 imex curation Botteaux A., Sani M., Kayath C.A., Boekema E.J., Allaoui A. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Botteaux A., Sani M., Kayath C.A., Boekema E.J., Allaoui A. Accepted 2013-FEB-01 by ORCHARD Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation span_shifl Surface presentation of antigens protein SpaN spaN spa32 CP0151 Spa32 protein protein protein shifl Shigella flexneri MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEYSLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQDIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSEMTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKGNKNNSTGYNEQSEEEC 78A112B3D24AB63C a1ju84_yere8 yscP YEP0026 protein protein yere8 Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) MNKITTRSPLEPEYQPLGKPHHDLQARVDFEQALLHNKDNRHPKEEPRRPVRPHDLGKKEGQKGDGLRAHAPLAATFQPGREVGLKPQHNHQNNHDFNLSPLAEGATNRKHLYQQDSRFDDRVESIINALMPLAPFLEGVTCETGTSSESPCEPFGHDELFVQQSPIDSAQPVQLNTKPTVQSLNPAADGAEVMIWSVGRDTLASIAKNQRDSRQKRLAEEPLPLHQEALPEVCPPAVSTTPDDHLVARWCATPVAEVAEKSARFPHKATVQSEQLDMTELADRSQHLTDGVDSSKDTIEPPRPEELLLPREETLPEMYSLSFTAPVITPGDHLLATMRATRLASVSEQLIQLAQRLAVELELRGGSSQVTQLHLNLPELGAIMVRIAEIPGKLHVELIASRETLRILAQGSYDLLERLQRIEPTQLDFQASGDSEQESRQKRHVYEEWEAEE 956C69FF51FC48ED q54046_saler spaN protein protein salmonella choleraes Salmonella choleraesuis MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSDDKKAREYGDAFMMHKETALPVLLAAWRHGVPAKSEHHNGNVAGLHHNGKGELRIAEKLLKVTAEKSVGLISAEAKVDKSAALLSSKNGQLEGVSGKKLSADLKAVESVSELGDKATGISDDNLKALPGDNKAIAGEGVRKEGALLARDVAPSRMAAANTSKPDDKDHKKIKDVSQLPLQPTTIADLSQLSGGDEKMPLAAQSKPMMTIFPTVDGVKGEDSSLTYRFQRWGNDYSVNIQARQVGEFSLIPSNTQVEHRLHDQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA 6D9C35959345AC94 q6xvw1_shifl spa40 protein protein shifl Shigella flexneri MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISFFSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKFVLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIFSQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH 47F0058AAEEA98B6 spa40-span 1 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined shifl Shigella flexneri 9 12 physical association physical association Fig. 4 spa40-span-3 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association Fig. 6E spa40-yscp 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6D spa40-span-2 2 3 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined shifl Shigella flexneri physical association physical association Fig. 4