mpidb The Microbial Protein Interaction Database Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. Mol. Microbiol. (0950-382X) 2009 imex curation El Qaidi S., Allemand F., Oberto J., Plumbridge J. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence El Qaidi S., Allemand F., Oberto J., Plumbridge J. Accepted 2013-FEB-01 by ORCHARD Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. Mol. Microbiol. (0950-382X) 2009 imex curation El Qaidi S., Allemand F., Oberto J., Plumbridge J. Only protein-protein interactions ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined El Qaidi S., Allemand F., Oberto J., Plumbridge J. Accepted 2013-FEB-01 by ORCHARD Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation galr_ecoli HTH-type transcriptional regulator GalR Galactose operon repressor galR b2837 JW2805 protein protein ecoli Escherichia coli (strain K12) MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDDRYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASHHATSD DIP protein P03024 original sequence version: null A4855FC8A2527A30 gals_ecoli HTH-type transcriptional regulator GalS Mgl repressor and galactose ultrainduction factor galS b2151 JW2138 protein protein ecoli Escherichia coli (strain K12) MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM F13D9DF41A29ADDA galr-gals 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Fig. 5A gals-galr 2 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig. 5A galr-galr 2 3 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Fig. 5A gals-gals 2 4 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig. 5A galr-gals-1 1 3 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal alexa 488 label alexa 488 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction Fig. 5B galr-galr-1 1 3 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 3 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal alexa 488 label alexa 488 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction Fig. 5B