UniProt UniProt http://www.uniprot.org Regulation of cyclin D1 RNA stability by SNIP1. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Res. (0008-5472) 2008 imex curation Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk Accepted 2013-JAN-11 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. Cancer Res. (0008-5472) 2008 Only protein-protein interactions imex curation Regulation of cyclin D1 RNA stability by SNIP1. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Res. (0008-5472) 2008 imex curation Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down ms participant Identification by mass spectrometry ms participant Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk Accepted 2013-JAN-11 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. Cancer Res. (0008-5472) 2008 Only protein-protein interactions imex curation Regulation of cyclin D1 RNA stability by SNIP1. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Res. (0008-5472) 2008 imex curation Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk Accepted 2013-JAN-11 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. Cancer Res. (0008-5472) 2008 Only protein-protein interactions imex curation Regulation of cyclin D1 RNA stability by SNIP1. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Res. (0008-5472) 2008 imex curation Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk Accepted 2013-JAN-11 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. Cancer Res. (0008-5472) 2008 Only protein-protein interactions imex curation Regulation of cyclin D1 RNA stability by SNIP1. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Res. (0008-5472) 2008 imex curation Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bracken C.P., Wall S.J., Barre B., Panov K.I., Ajuh P.M., Perkins N.D. n.d.perkins@bristol.ac.uk Accepted 2013-JAN-11 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. Cancer Res. (0008-5472) 2008 Only protein-protein interactions imex curation bclf1_human Bcl-2-associated transcription factor 1 BCLAF1 and THRAP3 family member 1 BCLAF1 BTF KIAA0164 protein protein human Homo sapiens Human MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRMYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRGRSSFYPDGGDQETAKTGKFLKRFTDEESRVFLLDRGNTRDKEASKEKGSEKGRAEGEWEDQEALDYFSDKESGKQKFNDSEGDDTEETEDYRQFRKSVLADQGKSFATASHRNTEEEGLKYKSKVSLKGNRESDGFREEKNYKLKETGYVVERPSTTKDKHKEEDKNSERITVKKETQSPEQVKSEKLKDLFDYSPPLHKNLDAREKSTFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSAAMTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKEYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDEEETMENNEEKKDRRKEEKE 8892B98E54F52C20 snip1_human Smad nuclear-interacting protein 1 SNIP1 FHA domain-containing protein SNIP1 protein protein human Homo sapiens Human MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRARGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPGGNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS B183F83EC3184676 pinin_human Pinin 140 kDa nuclear and cell adhesion-related phosphoprotein Domain-rich serine protein Melanoma metastasis clone A protein Desmosome-associated protein SR-like protein Nuclear protein SDK3 PNN DRS MEMA protein protein human Homo sapiens Human MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQRNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLEQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINKMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAEKEQEEEEQKQEMEVKMEEETEVRESEKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENEASKELEPEMEFEIEPDKECKTLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQSQPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDSSSSTSSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKRSSRSERDRKSDRKDKRR DIP protein Q9H307 original sequence version: 79 DIP protein Q9H307-1 original sequence version: 90 1F24D3F12E053C8F tr150_human Thyroid hormone receptor-associated protein 3 BCLAF1 and THRAP3 family member 2 BCLAF2 THRAP3 TRAP150 Thyroid hormone receptor-associated protein complex 150 kDa component protein protein human Homo sapiens Human MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSLSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQNRDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYSPRRGRSRSRSPKRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGTSQDTKASESSKPWPDATYGTGSASRASAVSELSPRERSPALKSPLQSVVVRRRSPRPSPVPKPSPPLSSTSQMGSTLPSGAGYQSGTHQGQFDHGSGSLSPSKKSPVGKSPPSTGSTYGSSQKEESAASGGAAYTKRYLEEQKTENGKDKEQKQTNTDKEKIKEKGSFSDTGLGDGKMKSDSFAPKTDSEKPFRGSQSPKRYKLRDDFEKKMADFHKEEMDDQDKDKAKGRKESEFDDEPKFMSKVIGANKNQEEEKSGKWEGLVYAPPGKEKQRKTEELEEESFPERSKKEDRGKRSEGGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGAKGDFPTGKSSFSITREAQVNVRMDSFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQHIVTIVHHVKEHHFGSSGMTLHERFTKYLKRGTEQEAAKNKKSPEIHRRIDISPSTFRKHGLAHDEMKSPREPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSAEKTEKTHKGSKKQKKHRRARDRSRSSSSSSQSSHSYKAEEYTEETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTFQFRARGRGWGRGNYSGNNNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSRGRGRGAFPRGRGRFMFRKSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDNIQPTTE 01131D2479B8C0F4 snw1_human SNW domain-containing protein 1 Nuclear protein SkiP Ski-interacting protein Nuclear receptor coactivator NCoA-62 SKIIP SKIP SNW1 protein protein human Homo sapiens Human MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGDGGAFPEIHVAQYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLVPKEVMNADDPDLQRPDEEAIKEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYTPSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE DIP protein Q13573 original sequence version: 156 0CC75E0D0B2CF842 snip1-bclaf1 2 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association 3A snip1-bclaf1-1 5 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 3B thrap3-bclaf1-2 1 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association 3C snip1-bclaf1-4 1 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association 3C snw1-bclaf1-2 1 10 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association 3C snip1-bclaf1-5 4 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association 3D thrap3-bclaf1-3 4 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association 3D bclaf1-snip1 4 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association 3D snip1-snw1 4 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association Suppl. 1A The association is not RNA-dependent (RNAse added prior to IP) thrap3-snw1 4 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Suppl. 1A The association is not RNA-dependent (RNAse added prior to IP) snip1-thrap3 3 9 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Suppl. 1D snip1-snw1-1 3 10 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Suppl. 1D