mpidb The Microbial Protein Interaction Database Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. J. Bacteriol. (0021-9193) 2008 imex curation Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. Accepted 2013-JAN-07 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. J. Bacteriol. (0021-9193) 2008 imex curation Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. Accepted 2013-JAN-07 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. J. Bacteriol. (0021-9193) 2008 imex curation Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli pull down pull down sequence tag sequence tag identification sequence tag Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. Accepted 2013-JAN-07 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation gtfb_strpa UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase stabilizing protein GtfB gtfB Glycosyltransferase chaperone Gtf2 Glycosyltransferase stabilizing protein GtfB Glycosyltransferase-stabilizing protein Gtf2 gtf2 protein protein streptococcus parasa Streptococcus parasanguis MIRLFEWLTQESLDLHYSLEESGIHGTSIVLNDDGFLPEGIISPYTFFCEVEMDGSPLYFNQLEVPYLWQITGTNIEGEIWNRSSKRGVIHYHEPKYLRFVQSVDWLYPDGSIYMTDHYNKYGWAFARTYFFSDQQVSHKKYYTKSGQEVLSENILTGDILLNWKGKVYHFTKKVDFFLFYFKKSGLDLSSIWYNSLGMPFLISYYLGGEGRDILFWQENLADQLPGNMQIIFSGRTSRTKKVIVQDRSVYKKLLHLVEEKNKEMISFLNIIYPKLRENYSRKEILIVTNSDQIEGIETLTDNLSAYTFHIGALTSMSDKLQNIGQKENVLLYPNMSPKTMLDLLEQCDIYLDINHGNEVLSIVRLAFERSLLILAYDNTVHSPIFHHESGIFNHSKPQTLSDWLLNLDDYSQTVSCWRSDLFPMTYRDYKQVLVSNVD 25FFBBF4D820C0FD gtfa_strpa UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit gtfA Glycosyltransferase Gtf1 Glycosyltransferase GtfA gtf1 protein protein streptococcus parasa Streptococcus parasanguis MTIYNINLGIGWASSGVEYAQAYRAQILRSLGMPAKFIFTNMFQSENLEHFTKNIGFEDNEIIWLYGYFTDVKISGTTYKKDDLEATFSQCPTKKEASSDRKLIRYYFENQELYINASLYGENQEYVQRVEYVVKGKLIRKDYYSYTKVFSEFYSPGENGVQLCNRSFYNEDGSIAYEEILSNEKSTFVFSNKICYGLEELLEFMLEDLSLTKSDLILLDRATGIGQVVFENIGAAKLAVVIHAEHFNEKNTDEHNILWNNYYEYQFTNADKVNAFITSTERQKILLEEQFTQYTSLHPKIVAIPVGSLDQLKFPEQSRKSFSMMTGSRLAIEKHIDWLIEGVALAQKRLPELTFDIYGEGGERRKLTELLTKLHAGEFIELKGHKQLDEIYQNYELYLTASTSEGFGLTLMEAVGSGLPIIGFDVPYGNQTFVCSGENGLLIERPKGDDRSRIVQAFADSIYEYFTKFKMADAQQYSYNIAENYKHEKLVERWKDFIEEMLND B0DDBA570024ED0E gtf1-gtf2 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 4A gtf2-gtf1 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 4A gtf1-gtf2-1 3 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5 gtf1-gtf2-2 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig. 4B gtf2-gtf1-1 1 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 5 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig. 4B gtf1-gtf2-3 1 4 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind insertion analysis insertion analysis range range range range region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association Fig. 6C