mpidb The Microbial Protein Interaction Database Dissection of a type VI secretion system in Edwardsiella tarda. Dissection of a type VI secretion system in Edwardsiella tarda. Mol. Microbiol. (0950-382X) 2007 imex curation Zheng J., Leung K.Y. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zheng J., Leung K.Y. Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2007 Only protein-protein interactions imex curation Dissection of a type VI secretion system in Edwardsiella tarda. Dissection of a type VI secretion system in Edwardsiella tarda. Mol. Microbiol. (0950-382X) 2007 imex curation Zheng J., Leung K.Y. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions edwta Edwardsiella tarda pull down pull down western blot western blot Immuno blot Immuno blot Zheng J., Leung K.Y. Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2007 Only protein-protein interactions imex curation Dissection of a type VI secretion system in Edwardsiella tarda. Dissection of a type VI secretion system in Edwardsiella tarda. Mol. Microbiol. (0950-382X) 2007 imex curation Zheng J., Leung K.Y. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions edwta Edwardsiella tarda anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zheng J., Leung K.Y. Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2007 Only protein-protein interactions imex curation q6ee19_edwta evpC protein protein edwta Edwardsiella tarda MAFDTYIKLDKVDGESTDDKHKKWIEVLGFAWGAGNECTMESGTQGLNTGKAMMSVLRVTKWMDCASVKLASAAVQGQNFPTLELEICTQAGDKFAFCIYKFTHVAVSSYQCSGATGGSDRPQETIDFAYKEVTWEYVPQDQNGKAGGKIGPEGWSLITNKKK 3AE68522FDAE5918 a8yqr8_edwta evpL protein protein edwta Edwardsiella tarda MSGSERWWKMTAPGGCSKVYWAMMKNKAIWSAIGCLLLSLSGCTSDAAKQQARSSVSWRYGSQAILVNAQTAPDLNSYDGESHSLVLAVVQTRSPDAFYQLIQTPELAGLVLQGGRPPDGILQVSRYALEPGHAFRLVLDRLQEARQVGFIAAFYGLPLTQTSRLFDIPVSVTRSGILFPDWQAAPSPLALDLKLGNSGIGEAESHPLERIPAAPDPASAPTAATPIRLSNSGEP 794B45AA3C0365FE q6ee21_edwta evpA protein protein edwta Edwardsiella tarda MASESKQHTLDRVRSPRVQITYDVEIGDAQEMKELPFVMGVLGDYSGQPATPLPKLKERKFVSIDRDNFNDVIKGVHPHLSFRTENTLSGDDSQLSVDLHFQSMADFTPERVAAQVEPLRKLMDIRSRLSDLKNKMYSNERLGEVLQGIIEDTEKLQSLGKETGFGEEKQP 92D9BBBA6CAE36A6 a8yqs1_edwta evpO protein protein edwta Edwardsiella tarda MAAMKRLFKWIAIVVLLSVLAMLCWLLTLWQRWPAAAAWVLFLAILAGALLTRYLWRKVRAWRTRSLEAEPVEKARAAERHPMTLQRQWKAATTLLKHSQLRRFRHPLQVLPWFMLLGRPGSGKSALLAQSGLAAPLDLPHELNDGKALVDWWYFDKAVILDISGQFVQPEASQETRSTWNRMLDLLGRDRARAGLNGLVLTVSAQTLLSADGGGVVDEGRLIRTRIEQLIRLFDRRFPVYVLVTQIDSLYGMEAWSHLLTPEALDQAMGHVVDEPENGRDVRDVIDIALGRVNSRLKTMQLTMLLQHPDADPALLMFPTELARLAEPLKRYVYHALGDSPYLESPILRGVFFASAVQKGNTRSGPEDQQEIALQEAAHDTVKRGVFVRDLFNRVLPADRHLNRPVAVTSPWRRLNRHLGLFAWWLCVAAALGYLGVSVVYWQHTLDDLRQRYPATMAMNRALPHDMQMMIAQRDAIYWLENRNAQWASRVLAFQHQLLSVEADAKSQYVNDFSQYILPSLDKAIQQSTTADSIASHPDGLVNAIQVEVRRANLLRARLRGSSYGALMAMPPIPTPPLRLLYPALDSDTVSRFNDLFLAQLAWSTDIQQQNARLLHLQARLRQLAAQSATLDWIIPWANAQPDLTNVTLDDFWQGSLRPDKLPEVDAAYTLEGQQRIRAFLVEMRSSMPNDIAFDKKRAAFWHEYAQKRLQAWQDFAGQFSQGERILAGQGEWQSTIDRLPHDDNPYLRLARRLLTEFAEMPDDLRPGWLRVLPQWLEIRQRAHQQALVRVPGLVSDVGGMILRGTAQKGALDQTRSQLVGRLDAIRLYQEYQAAMTQAMNSVVISQAQATKVAADFHGFDSQQSGQSSLQTAYARLMSLRSLLGYNRPDEQLTWGLMAGPLQLVIRYADQQASCFLQSAWQDRVIAPSRWTLNQAERADKLYGDNGLLWTFLSGPIKPFIQRNDSGWQEVDTLGQSVPFTADFLPFINRALIRQVDLKAGKIALHQKQQQATLAQQQQKQRWQTVAAESGEKLAELNTAAAALKAKSYPVALTGLPTDVNSNAKAKVVGTLLTMQCTNATLRLNNLNFAVMQSVLWSQQQCGQVTLQIRLPTFTLTRSYPGPQGFVHFLRDFPGGARRFTPADFPVDKSRMAAVGLTQITVRYRIAGQETLLQDAAQLAQAERDIQSAQTRQQQAQQALLTLNRPSASESLPPLDTLPGLTMMSVPERIGQCWRGDIRLARENDGIGRLMSELVAKQLSDKP FA058CCC8305D55D a8yqr4_edwta evpP protein protein edwta Edwardsiella tarda MSILNSELDWSHVGSISTGPGTVVSDAFNISYGLPTKELLPAGTALYKFNGFSSLARPPITDDTPLSPWWSPVQPFRHDGGLQQRMLVAKLNGVSMREWGRLTSVIKENWSSLDHLLEIVLKVPVYAWFGGFKGMSRIDNGMPSKRNITLEQKGRGSNLPGGATQFYIPNLTVGHISSHNFSALK C4499828877C314C a8yqs0_edwta evpN protein protein edwta Edwardsiella tarda MSLSEAFIQPMLYVRQYLQAPEGEPSLFRERLQSYLHHSQLRAQEAGESPAAIDSALYAVVAWMDETIMCSTWDGVATWRRDPLQASYFNTVCAGVDFFDKLTALSPEADDVREVYLLCLALGFEGRYAGPTGQTALTQIRVHQLRDLHGEVWEASRRLFPEAYPRSSVTPTQQTRWLTQSRFTTVVVPLVILVALYGILNWVLYEYADLALEWLP DFFC5704034805A8 evpp-evpc 3 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig. 4A evpo-evpn 1 7 unspecified role unspecified role bait bait region A8YQS1-444-1263 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region A8YQS0-1-182 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 17 physical association physical association Fig. 5C evpo-evpl 1 7 unspecified role unspecified role bait bait region A8YQS1-444-1263 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association evpo-evpa 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region A8YQS1-444-1263 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5 evpp-evpc-1 2 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association Fig. 4A