mpidb The Microbial Protein Interaction Database The N-terminal propeptide of Vibrio vulnificus extracellular metalloprotease is both an inhibitor of and a substrate for the enzyme. The N-terminal propeptide of Vibrio vulnificus extracellular metalloprotease is both an inhibitor of and a substrate for the enzyme. J. Bacteriol. (0021-9193) 2007 imex curation Chang A.K., Park J.W., Lee E.H., Lee J.S. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Chang A.K., Park J.W., Lee E.H., Lee J.S. Accepted 2012-DEC-17 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation c3vhn8_vibvl Neutral metalloproteinase vvpE protein protein vibvl Vibrio vulnificus MKHNQRHRLGLMIAAVVCSLPVTAAEMVSVSDSAVLEQALSVQARSLAPVENGFEAVKTIQLPNGKTKVRYQQTYLGLPVFDTAVVATEGRSGLSNVHGTMAQGLAADLPSVSVNLDQQHAIALGKQRHQAKHANAKALNTENETAQLMVRLDANNKAQLVYLVSFFVAEEEPSRPFMFIDANSGEILQVWDGLNHAQADGTGPGGNSKTGRYEFGTDYPSFVIDKVGTTCTMENSVVKTVDLQNRTSGSTAYSYSCPGASNYNDHKAVNGAYSPLNDAHYFGKVVYDMYKDWMNTAPLTFKLTMRVHYSSNYENAFWDGSAMTFGDGASTFYPLVDINVSAHEVSHGFTEQNSGLIYSNMSGGMNEAFSDIAGEAAEFYMKGSVDWIVGADIFKSNGGLRYFDQPSKDGRSIDHASQYYDGLNVHHSSGVYNRAFYLLANKTGWNVRKGFEIFTLANQLYWTANSTFDAGACGVVKAAQDMGYNSNDVAEAFNQVGVNANCGVTPPSGNVLKNNTPVSNLTGNKGSEVFYTFTVDRNATAVVSISGGSGDADLYLKAGSKPTTSSWDCRPYRYGNNESCSVSAAPGTTYHVMIKGYSNYSGVTLKLQY 99B88B83C932D713 vvpe-vvpe-2 1 2 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 2 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 5 physical association physical association Fig. 6, 7 The N-terminal propeptide inhibited the activities of both the mature protease plus the C-terminal propeptide, and only the mature protease but this could not be fully captured as the substrate used for assessing protease activity was a mixture of proteins.