<?xml version='1.0' encoding='UTF-8'?>
<entrySet xmlns="http://psi.hupo.org/mi/mif300" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/mi/mif300 https://raw.githubusercontent.com/HUPO-PSI/miXML/master/3.0/src/MIF300.xsd" level="3" version="0" minorVersion="0">
  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>mpidb</shortLabel>
        <fullName>The Microbial Protein Interaction Database</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
      </xref>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Differential interaction of Aggregatibacter (Actinobacillus) actinomycetemcomitans LsrB and RbsB proteins with autoinducer 2.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="17526716" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6400150" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-18496" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Differential interaction of Aggregatibacter (Actinobacillus) actinomycetemcomitans LsrB and RbsB proteins with autoinducer 2.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Bacteriol.   (ISSN:0021-9193)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Shao H, James D, Lamont RJ, Demuth DR</attribute>
            <attribute name="exp-modification" nameAc="MI:0627">Proteins incubated with AI-2  purified from  A. actinomycetemcomitans JP2.  Samples were then added to V. harveyi BB170 cells. Bioluminescence was determined at hourly intervals by luminometry</attribute>
            <attribute name="author-announcement">18-Dec-2012: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-18496" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="17526716" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="669">
            <names>
              <shortLabel>vibha</shortLabel>
              <fullName>Vibrio harveyi</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>affinity techniques</shortLabel>
            <fullName>affinity technology</fullName>
            <alias type="synonym" typeAc="MI:1041">affinity techniques</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0400" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608485" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Shao H, James D, Lamont RJ, Demuth DR</attribute>
          <attribute name="exp-modification" nameAc="MI:0627">Proteins incubated with AI-2  purified from  A. actinomycetemcomitans JP2.  Samples were then added to V. harveyi BB170 cells. Bioluminescence was determined at hourly intervals by luminometry</attribute>
          <attribute name="accepted">Accepted 2012-DEC-14 by ORCHARD</attribute>
          <attribute name="author-announcement">18-Dec-2012: Contacted by IntAct-Help.</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Bacteriol.   (ISSN:0021-9193)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>h0khb0_aggac</shortLabel>
          <alias type="orf name" typeAc="MI:0306">RHAA1_09361</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="H0KHB0" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="EHK89536" version="TrEMBL_20" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6400159" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="EHK89536" version="TrEMBL_20" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="RHAA1_09361" version="TrEMBL_20" refType="gene"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR025997"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR028082"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="1089447">
          <names>
            <shortLabel>aggregatibacter actinomycetemcomitans rhaa1</shortLabel>
            <fullName>aggregatibacter actinomycetemcomitans rhaa1</fullName>
          </names>
        </organism>
        <sequence>MDKFVRSQTDLKFKYADANGDPAKMLNDVENFIDSNVDALIIMPTDQKIVKAIGLKAKKAKIPLIVVTVKPNEEDMQYVASYVGSEEIKSGEMQGEFIVNSLNGKPAKVIILLGPLGLEAQIKRTEGNKTIFAQHPEIKVVAEQEAKWDRSKGMEVAENLLSAHRDANVILSNNDEMAIGALLAAKKLGFKDEDLLIVGIDATPDALGYLGNGLDATVYQSASGQGRLSAEMAYKAALGEDIPKYNWIPFELVTPDMKEQYINKYKE</sequence>
        <attributeList>
          <attribute name="crc64">85B5EF3CDCE41807</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>h0kca1_aggac</shortLabel>
          <fullName>S-ribosylhomocysteine lyase</fullName>
          <alias type="gene name" typeAc="MI:0301">luxS</alias>
          <alias type="gene name synonym" typeAc="MI:0302">AI-2 synthesis protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Autoinducer-2 production protein LuxS</alias>
          <alias type="orf name" typeAc="MI:0306">RHAA1_00125</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="H0KCA1" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="EHK91552" version="TrEMBL_42" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6400163" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="EHK91552" version="TrEMBL_42" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="RHAA1_00125" version="TrEMBL_42" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005506" version="TrEMBL_42"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009372" version="TrEMBL_42"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043768" version="TrEMBL_42"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR003815"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011249"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_005575894.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR037005"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="1089447">
          <names>
            <shortLabel>aggregatibacter actinomycetemcomitans rhaa1</shortLabel>
            <fullName>aggregatibacter actinomycetemcomitans rhaa1</fullName>
          </names>
        </organism>
        <sequence>MPLLDSFKVDHTRMKAPAVRIAKIMRTPKGDNITVFDLRFTIPNKENLPPKGIHTLEHLFAGFMRDHLNGKNVEIIDISPMGCRTGFYMSLIGTPNEAQVAQAWAASMQDILNVKKQSEIPELNEYQCGTYTEHSLEEAHQIAQNVLDRGIGVNKNEDLTLDESLLKQ</sequence>
        <attributeList>
          <attribute name="crc64">BC0D8D45F3533112</attribute>
        </attributeList>
      </interactor>
      <interactor id="4">
        <names>
          <shortLabel>h0kd38_aggac</shortLabel>
          <fullName>Autoinducer 2-binding protein LsrB</fullName>
          <alias type="orf name" typeAc="MI:0306">RHAA1_01359</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="H0KD38" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="EHK91255" version="TrEMBL_33" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6400177" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="EHK91255" version="TrEMBL_33" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="RHAA1_01359" version="TrEMBL_33" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042597" version="TrEMBL_33"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043190" version="TrEMBL_33"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR030159"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR025997"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR028082"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_005576281.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="1089447">
          <names>
            <shortLabel>aggregatibacter actinomycetemcomitans rhaa1</shortLabel>
            <fullName>aggregatibacter actinomycetemcomitans rhaa1</fullName>
          </names>
        </organism>
        <sequence>MKTRVKLTALSVLLGLSVVATAQAADRIAFIPKLVGVGFFTSGGQGATEMGKTLGVDVTYDGPTEPSVPNQVQLINNFVNQGYNAIVVSAVSPDGLCSTLQRAMKRGVKVLTWDSDTKPECRSHYINQGTPEQLGSMLVDMAANQISKPKAKVAFFYSSPTVTDQNQWVKEAKVKIAKDHPEWQIVITQFGYNDAIKSLQTAESILKAYPDLDAIIAPDANALPAAAQAAENLKVNSVVIVGFSTPNVMRPYVKRGTVKQFGLWDVVQQGKISVAAANTLLKGEKLNVGDKLNVQGIGTIEVSPNKVQGYEYEADGNGIILLPERVVFTKDNIDNYNF</sequence>
        <attributeList>
          <attribute name="crc64">5967C724B384DF2C</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="5" imexId="IM-18496-1">
        <names>
          <shortLabel>h0khb0-luxs-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-2074" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6400167" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-18496-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="6">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="7">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">figure 3</attribute>
        </attributeList>
      </interaction>
      <interaction id="8" imexId="IM-18496-2">
        <names>
          <shortLabel>h0kd38-luxs-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-2073" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6400173" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-18496-2" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="9">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="10">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">figure 2</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>