UniProt UniProt http://www.uniprot.org AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. Genes Cells (1356-9597) 2006 imex curation Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp Accepted 2012-JUL-24 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Genes Cells (1356-9597) 2006 Only protein-protein interactions imex curation AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. Genes Cells (1356-9597) 2006 imex curation Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. pull down pull down sequence tag sequence tag identification sequence tag Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp Accepted 2012-JUL-24 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Genes Cells (1356-9597) 2006 Only protein-protein interactions imex curation AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. Genes Cells (1356-9597) 2006 imex curation Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp Accepted 2012-JUL-24 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Genes Cells (1356-9597) 2006 Only protein-protein interactions imex curation AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. Genes Cells (1356-9597) 2006 imex curation Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp Accepted 2012-JUL-24 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Genes Cells (1356-9597) 2006 Only protein-protein interactions imex curation AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. Genes Cells (1356-9597) 2006 imex curation Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 imaging technique imaging technique microscopy immunostaining immunostaining Ishizaki R., Shin H.W., Iguchi-Ariga S.M., Ariga H., Nakayama K. Origin of MYCBP/AMY-1 according pmid 9797456; origin of ARFGEF1/BIG2 according pmid 11777925 hiro@pharm.hokudau.ac.jp Accepted 2012-JUL-24 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Genes Cells (1356-9597) 2006 Only protein-protein interactions imex curation arfgef1_crigr_protein protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to mouse G3X9K3 UniProt entry big2_human Brefeldin A-inhibited guanine nucleotide-exchange protein 2 ARFGEF2 ARFGEP2 BIG2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 protein protein human Homo sapiens Human MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW DIP protein Q9Y6D5 original sequence version: 124 DIP protein Q9Y6D5 original sequence version: 89 DIP protein Q9Y6D5 original sequence version: 109 D419106E5BAF19C2 hsp7c_crigr Heat shock cognate 71 kDa protein HSPA8 HSC70 Heat shock 70 kDa protein 8 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGEAKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRNDKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD FC65A2DC5095AD1C g3hm63_crigr I79_011811 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEQSYGGYGAWSAAPANTQGAYGSGMASWQGYENYNYYNAQNTTATVSAGAPYSYGPTSWEATKASDGGLAAGNSAMQMPSFAPEPCTDNSDTLIAKINQRLDMLSKEGGRGGASSSGEGMQDRDSSFRFQSYEPYDSRPCLPEHNPYRPSYSYDYDFDLGADRNGTFGGTFNDCRDPTPERGSLDGFMRGRGQGQGQGQGRFQDRSNSNTFIRSDPFMPPSASSEPLPTTWNELNYMGGRGLGGPSTNRPPPSLFSQSMAADYSMMGVQGVGTFGSTMPYGYGRSQTRIRDWPRRRGFERFGPDNMGRKRKQFPMYEEPDAKLARADSDGDLSENDDGAGDLRSGDEEFRGEDELYDSRKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQHQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFASETGDLETEGDENVGGEKEETPEEVAAEVLAEVITAAVRAVEGEGEPAAEHNEVPDEVEGPVNTMETSSDRHTEKPLEERTCETAPEDGNPEDKARSEAVMPAAETESTIPVTAIPGIMDDDGVGQTDADSKDTPTE similar to mouse Q9DBR0 UniProt entry E61838874740B7D2 big1_human Brefeldin A-inhibited guanine nucleotide-exchange protein 1 ARFGEF1 ARFGEP1 BIG1 p200 ARF-GEP1 p200 ARF guanine nucleotide exchange factor ADP-ribosylation factor guanine nucleotide-exchange factor 1 protein protein human Homo sapiens Human MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ DIP protein Q9Y6D6 original sequence version: 124 DIP protein Q9Y6D6 original sequence version: 67 DIP protein Q9Y6D6 original sequence version: 87 DIP protein Q9Y6D6 original sequence version: 108 1EB2547F28F63BCA mycbp_human c-Myc-binding protein Associate of Myc 1 MYCBP AMY1 protein protein human Homo sapiens Human MAHYKAADSKREQFRRYLEKSGVLDTLTKVLVALYEEPEKPNSALDFLKHHLGAATPENPEIELLRLELAEMKEKYEAIVEENKKLKAKLAQYEPPQEEKRAE 72B9BD179A6808D0 arfgef2_crigr_protein protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to mouse A2A5R2 UniProt entry arfgef2-mycbp-1 1 11 biological role biological role prey prey 7 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9Y6D5:p.Ile292Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I P Wed May 02 14:35:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile292pro' Tue Jun 30 20:24:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile292Pro' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2C arfgef1-mycbp 1 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey physical association physical association 2C mycbp-arfgef2 4 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3A arfgef2-mycbp-2 4 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3A mycbp-arfgef2-1 3 7 unspecified role unspecified role prey prey cell lysate cell lysate 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B mycbp-arfgef1 3 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey cell lysate cell lysate physical association physical association 3B arfgef2-mycbp-3 5 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization 4B-B'' arfgef1-arfgef2-3 5 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 4A-A'' mycbp-akap8-1 2 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association 2A