mbinfo MBInfo http://www.mechanobio.info/ WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP. WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP. Biochem. Biophys. Res. Commun. (0006-291X) 2006 imex curation Rajmohan R., Meng L., Yu S., Thanabalu T. Interaction detection method: GST pull down. The source of WASP and WIP specified by the author. thirumaran@ntu.edu.sg 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rajmohan R., Meng L., Yu S., Thanabalu T. Interaction detection method: GST pull down. The source of WASP and WIP specified by the author. thirumaran@ntu.edu.sg Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Biophys. Res. Commun. (0006-291X) 2006 Only protein-protein interactions imex curation WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP. WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP. Biochem. Biophys. Res. Commun. (0006-291X) 2006 imex curation Rajmohan R., Meng L., Yu S., Thanabalu T. Interaction detection method: GST pull down. The source of WASP and WIP specified by the author. thirumaran@ntu.edu.sg 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Rajmohan R., Meng L., Yu S., Thanabalu T. Interaction detection method: GST pull down. The source of WASP and WIP specified by the author. thirumaran@ntu.edu.sg Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochem. Biophys. Res. Commun. (0006-291X) 2006 Only protein-protein interactions imex curation wasp_human Wiskott-Aldrich syndrome protein WAS IMD2 protein protein human Homo sapiens Human MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD DIP protein P42768 original sequence version: 183 DIP protein P42768 original sequence version: 168 DIP protein P42768 original sequence version: 132 DIP protein P42768 original sequence version: 155 7228428672B7CB78 wipf1_human WAS/WASL-interacting protein family member 1 WIPF1 WIP Wiskott-Aldrich syndrome protein-interacting protein Protein PRPL-2 WASPIP protein protein human Homo sapiens Human MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSPHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPPIPR DIP protein O43516 original sequence version: 128 DIP protein O43516 original sequence version: 97 DIP protein O43516 original sequence version: 131 09B7A95E6808A46D wipf1-was-4 2 3 unspecified role unspecified role unspecified role unspecified role wh1 domain required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range The amino acid sequence for the WH1 domain was taken from UniProt. region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P42768:p.Ala134Thr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A T Wed May 02 19:13:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala134thr' Tue Jun 30 16:01:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala134Thr' 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4A. was-wipf1-2 1 4 unspecified role unspecified role prey prey wasp-binding domain required to bind necessary binding region required to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait physical association physical association Table 1.