IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Nat. Cell Biol. (1465-7392) 2005 imex curation Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu Accepted 2012-FEB-14 by ORCHARD Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Nat. Cell Biol. (1465-7392) 2005 imex curation Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. The molecule "worm alpha-adaptin/apt-4 (T20G5.1)" has been entered as: T20B5.1, as the ORF T20G5.1 corresponds to chc-1 rather than Apt-4. grant@biology.rutgers.edu Accepted 2012-FEB-14 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Nat. Cell Biol. (1465-7392) 2005 imex curation Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu Accepted 2012-FEB-14 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Nat. Cell Biol. (1465-7392) 2005 imex curation Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. The molecule "worm alpha-adaptin/apt-4 (T20G5.1)" has been entered as: T20B5.1, as the ORF T20G5.1 corresponds to chc-1 rather than Apt-4. grant@biology.rutgers.edu Accepted 2012-FEB-14 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Nat. Cell Biol. (1465-7392) 2005 imex curation Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. The molecule "worm alpha-adaptin/apt-4 (T20G5.1)" has been entered as: T20B5.1, as the ORF T20G5.1 corresponds to chc-1 rather than Apt-4. only ONE co-loc entered as this interaction is valideated by co-IP - others are not. grant@biology.rutgers.edu Accepted 2012-FEB-14 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation rme6_caeel Receptor-mediated endocytosis protein 6 rme-6 F49E7.1 protein protein caeel Caenorhabditis elegans MADIIERIRSQDEYREFYELATKIRNQKLLTDAELHSLNRHQQEVSDNEIKLLTEAWRSSYWNNVGRQLKNQALEPTIACRLLKEISETEALPAYKHVGHHFSTLDQLLTILYNEPQSVAELLNSIDRNDITSNDSTIQVLFHLVYSCALYPEDELKIAQVVCNLLKLQLYRSGSEMRTILRKETSISTRFYKHFVELIHPTMVYLTKALRKGVLDVIQLGSFWLDIDAKQSTARFLRDNKQNEDRLPEYRALVVSKLVDFVDTFLENISKALPMLPPNLNWIIRDIFCSLYEVVDDVSAIELSHACKDMIVSNLICPAIISPQKFGVVDNDVRVGTIVNHNLVQIAMIIQMISLREFESPPEEYKEFLSQCRNTHLISEMMDALLVEKLAPDVEITSLIANGTAESDMATKSSFVGTVADVNVLIKIIRNAPSTSDAKLSRILSVCDKLPATISSTTQKSHFDNLEASPKISTLRNIHRKVQHSFKRTGDSFNDINELGAKQGEALAFGKENFDLFYLEYSPKNYKDVAEADRLKKKAEERRQLADLEKLLEPTPAPKETPVENNLIDFSSGSAETNSEHLSDSTSVSPEPLTSTEELQIGDQGVGGEQRGKLAKLKSLSDRMKKGITQSNTLSDIREHLRRSGSFVKPPPSGIPTSSSEQNLPDVATGPRDDILAKYASISSIKEQKPALGNLIDGTASASSTPRKEPLEPYYSSENLVGCRAFQDTLRKMITVLGNISYLPRIGYRNETKQNEWDKKVLLGRFLDGVLVETEHRREYGLAAQLREVKRCIELFEHAGVEILMDHLKLNEAEQDKIVNGMREERASLMRKSNDISSLEQRVLLNRRLTEQILVDFLMRTFLETGFNNKHTVGKTQEVTAVLKFYDEFKYLRAQDERAEFLKNLLTFLRDRLMQNVDWNFATDTMMSRAMTTIERYVIFAVYDNAFYPNRDADHHRDKLLRGTIAKVSDVVTPVNDFLKIPEHLHGEAPWPSAQAELSMLDIYVTAQDKLNCVVRCCDVINNLVALSSKNAVASADDLTPVLVFVIIKANPRALLSNVQFVETFAGDRIESGRDAYYWVNFKSAVEYIKTIL 8C50161C988EF4B2 q8i100_caeel rabx-5 CELE_Y39A1A.5 Y39A1A.5 protein protein caeel Caenorhabditis elegans MSDKHGEEDKKAKMHLKANDLLCVNGCGFYGTPQWENRCSKCWRAHQNEMKKCQDFAKNRSLLSFDQFQERRKSTTESKSRGIKNLFKTSPIPEGGTSSPTSTSPASTPTRRRELSPDSLEARQQFTDFLVANLSTGMAQEIARSVKNAVNKISEMRMSSDDMSELVMSYYQYLGERIGGHSLFDSPDCKVKVEDVMDQVEKYISTFCYSIFFCANHEEEVADMSLQDRIRSLHWVTAGFLETKMVFKKQTVRDKIDEAISELIEINAKRSAFEKLDCLTKSCKAIFEALKESEASTSADEFLPTLIYVLFRGNPPLIQSNVKFISRFAIPARLMSGEAAYFFTNLSCALEFARNMNHESLQMEKSEFEAYTSGHLAPPLSVINSACNQAIYVLEGTIETITNVAKKAGSLAKNLTNMHGKSDDDLEKMLAVIKETVDFYPTDEYMNMKQSIFAEEKETADILVSLSRQSSESGRGTLTDTQPSTPQPDTSLESPLIAEIQAINLTSSGNQEHVEEA B884B6A67CD06CED q19088_caeel rabn-5 F01F1.4 CELE_F01F1.4 protein protein caeel Caenorhabditis elegans MTEPSASEEVSAGVNAEEPPIPSKKISQVSPNPTLQCEMCQNYEVNLTALQENERKLKEEVKAAMELNERYQLDLSNERDYRKELEKKMNELSTNCLNQVNEAAKANAKSEQRLDELSAKHDKSIEVFTDQLNLARDKLQQADDDFVTLGKKYKKLLGSTRKSAQELRSEKIDMPADLDQLQFICLKNREELIETRAAKEHTEQQLQDEITVLRAQILEERAHREDQEVTFTAEISRLQSELGKINTRMVVASETIADQDSNIRQIRDLQTTVGELETQVQQVQNERAAVEQTAQNYKQRCTSLQQELDTSEVVQRDFVKLSQSLQIQLEKIRQSEQEVRWQWDDDVDQCSNCDTSFARVKVKPHCLHCGRIFCMNCLKDTVPSGPNHRPANVCKVCHTLLNRDTKPFFATQDK 6F4D5F5BBB46F316 q22601_caeel AP-2 complex subunit alpha T20B5.1 apa-2 CELE_T20B5.1 protein protein caeel Caenorhabditis elegans MPAVKGDGMRGLAVFISDIRNCKSKEAELKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGNDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLIEQQSDLMKLIVQGIRNDLTSRNPVHVNLALQCISNMGSREMVEAFCTDLPKLLVSGETIDFVKQSAALCILKLFRNSPDSFQPGDYASRIVHLLNDSHMGVVTSAASLIEALSKKWPEEYKGAVPLAISRLSRIVTATYTDLQDYTYYFVPAPWLCVKLLRLLQNYPPPDDPSNKARLLECLEGILNKAQDAPKSKKVQHSNAKNAVLFEAIALIIHMDSEPQLLVRACNQLGTFLSHRETNLRYLALESMCLLATSEFSHDAVKKHQDTIINSLKTERDVSVRQRAVDLLYAMCDRSNANQIVAEMLAYLETADYSIREEMVLKVAILAEKYATDYTWYVDVILKLIRIAGDYVSEEVWYRVIQIVVNREDVQGYAAKTVFEALQRPACHENMVKVGGYILGEFGNFIAGDERSTAKIQFELLHSKFHLCSITTRCLLLTTYIKFCNLFPEIKPLVQQVFQTDHNLRNPDAELQQRSIEYLQMTKLASNDVLATILEVMPAFAEKESSLLAKLKKSKPQLEEIEREEKEKRSKPTAVMSEGSTSLVDFDSTNDTTASLADVFANNSGTGLGAQGDEVEIANKDDYLKFVTKSNAILWEDDYIQIGCKLETRNNLGRLGMFYGNKTSQPFNKFTPIITCPGALAVQLQAQAKPVEPVVAAGTQVQQLINFVCVQEFQKMPIMNIKFTFTDRAGAVQNFDKNFYLPLFISKFFEPTNMTSEQFFTRWKSLGAASQEAQKIFNALSPIEHATIESRLKGFGANLLTDVDPNPDNYVCAGIIHTQTQQIGTLIRLEPNKQAKMYRLTIRSSKDTVVQTLVDLLGNQF A816ADC042FD557C p91857_caeel rab-5 CELE_F26H9.6 F26H9.6 protein protein caeel Caenorhabditis elegans MAARNAGTARPGGPNRTCQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDATIKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQESFQKAKNWVKELQRQASPNIVMALAGNKADVANKRTVEYEEANAYAEDNALLFMETSAKTSMNVNDIFMAIAKKLPIGPAQGEPTGTVDMNQPQQQQKGSCCK 3DCD9DA1716AB992 rme6-rab5 1 6 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey P91857:p.Ser33Asn mutation mutation certain certain sequence position certain certain certain sequence position certain S N Wed May 02 16:55:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33asn' Tue Jun 30 18:55:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser33Asn' physical association physical association Fig 2 RME-6 specifically interacted with RAB-5(S33N). rabx5-rab5 1 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplementary Fig 2 rabx5-rabn 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Supplementary Fig 2 rme_6-g5ed80 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 8e rab5-rme6-2 3 10 unspecified role unspecified role bait bait P91857:p.Ser33Asn mutation mutation certain certain sequence position certain certain certain sequence position certain S N Wed May 02 18:52:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33asn' Tue Jun 30 17:32:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser33Asn' n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 2 RME-6 specifically interacted with RAB-5(S33N). apa_2-rme_6-2 4 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 8f apa_2-rme_6-1 5 6 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig 7b