mbinfo MBInfo http://www.mechanobio.info/ An interaction between zyxin and alpha-actinin. An interaction between zyxin and alpha-actinin. J. Cell Biol. (0021-9525) 1992 imex curation Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay autoradiography autoradiography Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1992 Only protein-protein interactions imex curation An interaction between zyxin and alpha-actinin. An interaction between zyxin and alpha-actinin. J. Cell Biol. (0021-9525) 1992 imex curation Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1992 Only protein-protein interactions imex curation An interaction between zyxin and alpha-actinin. An interaction between zyxin and alpha-actinin. J. Cell Biol. (0021-9525) 1992 imex curation Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1992 Only protein-protein interactions imex curation zyx_chick Zyxin ZYX protein protein chick Gallus gallus (Chicken) Chicken MASPGTPGTRMTTTVSINISTPSFYNPQKKFAPVVAPKPKVNPFKTGGTSESSQPQPPGTGAQRAQIGRVGEIPVSVTAEELPLPPPPPPGEELSFSSNCAFPPPPPPFEEPFPPAPDEAFPSPPPPPPPMFDEGPALQIPPGSTGSVEKPLAPKAHVEISSAPRDPTPPFPSKFTPKPSGTLSSKPPGLDSTPAPAPWAAPQQRKEPLASVPPPPSLPSQPTAKFTPPPVASSPGSKPGATVPMAPSNSTRYPTSLQTQFTAPSPSGPLSRPQPPNFTYAQQWERPQVQEKPVPTEKSAAVKDMRRPTADPPKGNSPLTMKEVEELELLTQKLMKDMDHPPPVEAATSELCGFCRKPLSRTQPAVRALDCLFHVECFTCFKCEKQLQGQQFYNVDEKPFCEDCYAGTLEKCSVCKQTITDRMLKATGNSYHPQCFTCVMCHTPLEGASFIVDQANQPHCVDDYHRKYAPRCSVCSEPIMPEPGKDETVRVVALEKNFHMKCYKCEDCGRPLSIEADENGCFPLDGHVLCMKCHTVRAKTAC 9D898AC180C680FC p05094-2 Alpha-actinin-1 Smooth muscle ACTN1 Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1 protein protein chick Gallus gallus (Chicken) Chicken MDHHYDPQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHESWTDGKEAMLQQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERQAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWEHVRQLVPRRDQALMEEHARQQQNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDCEDFRACLISMGYNMGEAEFARIMSIVDPNRMGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYNGRDAVPGALDYMSFSTALYGESDL 5F2485A047EA644C actn1-zyx 3 actn1 5 unspecified role unspecified role neutral component neutral component chick-gizzard Chicken gizzard gizzard gizzard 4 unspecified role unspecified role neutral component neutral component chick-gizzard Chicken gizzard gizzard gizzard physical association physical association Fig. 2B zyx-actn1 2 4 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick-gizzard Chicken gizzard gizzard gizzard actn1 5 unspecified role unspecified role prey prey chick-gizzard Chicken gizzard gizzard gizzard physical association physical association Fig. 3B& 4B Purified alpha-actinin was used in Fig 3B, while a mixture of proteins from chick gizzard extracts was used in Fig 4B. Zyxin binds to the 27kDa fragment of alpha-actinin-1, but not the 53 kDa fragment.(Fig.3B) actn1-zyx 2 actn1 5 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick-gizzard Chicken gizzard gizzard gizzard 4 unspecified role unspecified role prey prey chick-gizzard Chicken gizzard gizzard gizzard physical association physical association Fig. 5B The heat denatured alpha-actinin does not bind zyxin. zyx-actn1 1 4 unspecified role unspecified role bait bait chick-gizzard Chicken gizzard gizzard gizzard actn1 5 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick-gizzard Chicken gizzard gizzard gizzard physical association physical association Fig. 6 & 7A,B The association between zyxin and 125 i-alpha-actinin was competitively inhibited by unlabeled alpha-actinin (Fig. 6&7a).