mbinfo MBInfo http://www.mechanobio.info/ Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions human-melanoma Human melanoma melanoma Melanoma fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions bovin-pulmonary_artery Bos taurus pulmonary artery cells pulmonary_artery fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation Myosin-X provides a motor-based link between integrins and the cytoskeleton. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. (1465-7392) 2004 imex curation Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Only protein-protein interactions bovin-pulmonary_artery Bos taurus pulmonary artery cells pulmonary_artery anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang H., Berg J.S., Li Z., Wang Y., Lang P., Sousa A.D., Bhaskar A., Cheney R.E., Stromblad S. Interaction detection: Yeast two hybrid screening. The source of integrin beta-5 and myosin 10 specified by the author. hongquan.zhang@labmed.ki.se Accepted 2013-JAN-04 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mouse embryo cDNA Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation itav_bovin Integrin alpha-V ITGAV Vitronectin receptor subunit alpha protein protein bovin Bos taurus (Bovine) Bovine MAFPPRRRLRLGPRGLPLLLSGLLLPLCRAFNLDVESPAEYSGPEGSYFGFAVDFFVPSASSRMFLLVGAPKANTTQPGIVEGGQVLKCDWSSHRRCQPIEFDATGNRDYAKDDPLEFKSHQWFGASVRSKQDKILACAPLYHWRTEMKQEREPVGTCFLQDGTKTVEYAPCRSKNIDADGQGFCQGGFSIDFTKADRVLLGGPGSFYWQGQLISDQVAEIVSKYDPKVYSIKYNNQLATRTAQAIFDDSYLGYSVAVGDFNGDGIDDFVSGVPRAARTLGMVYIYDGKNMSSLHNFTGEQMAAYFGFSVAATDINGDDYADVFIGAPLFMDRGSDGKLQEVGQVSVSLQKASGDFQTIKLNGFEVFARFGSAIAPLGDLDQDGFNDIAIAAPYGGEDKKGIVYIFNGRPTGLNAVPSQILEGKWAARSMPPSFGYSMKGATDIDKNGYPDLIVGAFGVDRAVLYRARPVITVNAGLEVYPSILNQENKTCPLPGTDLKVSCFNVRFCLKADGKGALPTKLDFQVELLLDKLKQKGAIRRALFLHNRSPGHSKNMTISRGGQMQCEELIAYLRDESEFRDKLTPITIFMEYWLDYRTAADATGLQPILNQFTPANVSRQAHILLDCGEDNVCKPKLEVSVDSDQKKIYIGDDNPLTLIVKAQNQGEGAYEAELIVSIPLQADFIGVVRNSEALARLSCAFKTENQTRQVVCDLGNPMKAGTQLLAGLRFSVHQQSEMDTSVKFDLQIQSSNLFDKVSPVVSYKVDLAVLAAVEIRGVSSPDHIFLPIPNWKYKENPETEEDVGPVVQHIYELRNNGPSSFSKAMLHLQWPYKYNNNTLLYILQYDIDGPMNCTSDMEINPLRIKISNSQTSEKNDTVGGQGDRNHLITKRDLTLNEGDVHTLGCGIAECLKIVCQVGRLDRGKSAILYVRSLLWTETFMNKENQNHSYSLKSSASFNVIEFPYKNLPIEDIFNSTLVTTNVTWGIQPAPMPVPVWVIILAVLAGLLLLAVLVFVMYRMGFFKRVRPPQEEQEREQLQPHENGEGNSET A6A5620EB3144502 myo10_mouse Unconventional myosin-X Myo10 Unconventional myosin-10 protein protein mouse Mus musculus Mouse MDSFFPEGARVWLRENGQHFPSTVNSCAEGVVVFQTDYGQVFTYKQSTITNQKVTAMHPLHEEGVDDMASLAELHGGSIMYNLFQRYKRNQIYTYIGSIIASVNPYQPIAGLYERATMEEYSRCHLGELPPHIFAIANECYRCLWKRHDNQCVLISGESGAGKTESTKLILKFLSVISQQTLDLGLQEKTSSVEQAILQSSPIMEAFGNAKTVYNNNSSRFGKFVQLNICQQGNIQGGRIVDYLLEKNRVVRQNPGERNYHIFYALLAGLDQGEREEFYLSLPENYHYLNQSGCTEDKTISDQESFRQVITAMEVMQFSKEEVREVLRLLAGILHLGNIEFITAGGAQIPFKTALGRSADLLGLDPTQLTDALTQRSMILRGEEILTPLSVQQAVDSRDSLAMALYARCFEWVIKKINSRIKGKDDFKSIGILDIFGFENFEVNHFEQFNINYANEKLQEYFNKHIFSLEQLEYSREGLVWEDIDWIDNGECLDLIEKKLGLLALINEESHFPQATDSTLLEKLHSQHANNHFYVKPRVAVNNFGVKHYAGEVQYDVRGILEKNRDTFRDDLLNLLRESRFDFIYDLFEHVSSRNNQDTLKCGSKHRRPTVSSQFKDSLHSLMATLSSSNPFFVRCIKPNTQKMPDQFDQVVVLNQLRYSGMLETVRIRKAGYAVRRPFQDFYKRYKVLMRNLALPDDIRGKCTVLLQVYDASNSEWQLGKTKVFLRESLEQKLEKRREEEIDRAAMVIRAHILGYLARKQYRKVLCGVVTIQKNYRAFLARKKFLHLKKAAIVFQKQLRGQLARRVYRQLLAEKRELEEKKRREEEKKREEEERERERAQREADLLRAHQEAETRRQQELEALQKSQREADLTRELEKQRENKQVEEILRLEKEIEDLQRMKERQELSLTEASLQKLQQLRDEELRRLEDEACRAAQEFLESLNFDEIDECVRNIERSLSVGSEISGEELSELAESASGEKPNFNFSQPYPAEEEVDEGFEADDDAFKDSPNPSEHGHSDQRTSGIRTSDDSSEEDPYMNYTVVPTSPSADSTVLLAASMQDSASLHNSSSGESTYCMPQNNGDLPSPDGDYDYDQDDYEDGAITSGSSVTFSNSYGSQWSPDYRYSVGTYNSSGAYRFSSEGAQSSFEDSEEDFDSRFDTDDELSYRRDSVYSCVTLPYFHSFLYMKGGLMNSWKRRWCVLKDETFLWFRSKQEALKQGWLHKKGGGSSTLSRRNWKKRWFVLRQSKLMYFENDSEEKLKGTVEVRTAKEIIDNTSKENGIDIILADRTFHLIAESPEDASQWFSVLSQVHSSTDQEIREMHDEQANPQNAVGTLDVGLIDSVCASDSPDRPNSFVIITANRVLHCNADTPEEMHHWITLLQRSKGDTRVEGQEFIVRGWLHKEVKNSPKMSSLKLKKRWFVLTHNSLDYYKSSEKNALKLGTLVLNSLCSVVPPDEKIFKETGYWNVTVYGRKHCYRLYTKLLNEATRWSSAIQNVTDTKAPIDTPTQQLIQDIKENCLNSDVVEQIYKRNPILRYTHHPLHSPLLPLPYGDINLNLLKDKGYTTLQDEAIKIFNSLQQLESMSDPIPIIQGILQTGHDLRPLRDELYCQLIKQTNKVPHPGSVGNLYSWQILTCLSCTFLPSRGILKYLKFHLKRIREQFPGTEMEKYALFIYESLKKTKCREFVPSRDEIEALIHRQEMTSTVYCHGGGSCKITINSHTTAGEVVEKLIRGLAMEDSRNMFALFEYNGQVDKAIESRTIVADVLAKFEKLAATSEAGDAPWKFYFKLYCFLDTDSMPKDSVEFAFMFEQAHEAVIHGHHPAPEESLQVLAALRLQYLQGDYTPHTSIPPLEEVYSVQRLRARISQSTKTFTPYERLEKRRTSFLEGTLRRSFRTGSVVRQKAEEEQMLDMWIKEEVCSARTSIIDKWKKLQGMNQEQAMAKYMALIKEWPGYGSTLFDVECKEGGFPQELWLGVSADAVSVYKRGEGKPLEVFQYEHILSFGAPLANTYKIVVDERELLFETSEVVDVAKLMKAYISMIVKKRYSTTRSVSSQGSSR FD685BEE8B88DE30 itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C itb3_human Integrin beta-3 Platelet membrane glycoprotein IIIa ITGB3 GP3A protein protein human Homo sapiens Human MRARPRPRPLWATVLALGALAGVGVGGPNICTTRGVSSCQQCLAVSPMCAWCSDEALPLGSPRCDLKENLLKDNCAPESIEFPVSEARVLEDRPLSDKGSGDSSQVTQVSPQRIALRLRPDDSKNFSIQVRQVEDYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEALENPCYDMKTTCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVNLYQNYSELIPGTTVGVLSMDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCMGLKIGDTVSFSIEAKVRGCPQEKEKSFTIKPVGFKDSLIVQVTFDCDCACQAQAEPNSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPVCSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGALHDENTCNRYCRDEIESVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT DIP protein P05106 original sequence version: 202 DIP protein P05106-1 original sequence version: 158 DIP protein P05106-1 original sequence version: 164 DIP protein P05106 original sequence version: 200 DIP protein P05106-1 original sequence version: 213 DIP protein P05106-1 original sequence version: 175 DIP protein P05106-1 original sequence version: 192 DIP protein P05106-1 original sequence version: 171 F246623608E05F9E f1mtn1_bovin Integrin beta ITGB3 protein protein bovin Bos taurus (Bovine) Bovine MRARRLWAAVLVVGALAGVGVGGPNICATRGVSSCQQCLAVSPTCAWCSDEALPPGSPRCNLKENLLKDNCHPESIEFPISEARILEARPLSDKGTGDSSQITQVSPQRIALRLRPDDSKIFSVQVRQVEDYPVDIYYLMDLSYSMKDDLRNIQNLGTKLASQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEAIRNPCYDMKATCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTESVVNLYQNYSELIPGTTVGVLSTDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCVGLKIGDTVSFSIEAKVRGCPQKKEKSFTIKPVGFKDSLTVQVTFDCDCACQAEAEPFSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPICSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFNRGALNEENTCNRYCRDEIEPVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLATLLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT F56B6F4269B780A1 itb5_human Integrin beta-5 ITGB5 protein protein human Homo sapiens Human MPRAPAPLYACLLGLCALLPRLAGLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANLVKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTFQLQVRQVEDYPVDLYYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFGSFVDKDISPFSYTAPRYQTNPCIGYKLFPNCVPSFGFRHLLPLTDRVDSFNEEVRKQRVSRNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDVPHIALDGKLGGLVQPHDGQCHLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTALIPGTTVEILDGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGDTASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECSPGYLGTRCECQDGENQSVYQNLCREAEGKPLCSGRGDCSCNQCSCFESEFGKIYGPFCECDNFSCARNKGVLCSGHGECHCGECKCHAGYIGDNCNCSTDISTCRGRDGQICSERGHCLCGQCQCTEPGAFGEMCEKCPTCPDACSTKRDCVECLLLHSGKPDNQTCHSLCRDEVITWVDTIVKDDQEAVLCFYKTAKDCVMMFTYVELPSGKSNLTVLREPECGNTPNAMTILLAVVGSILLVGLALLAIWKLLVTIHDRREFAKFQSERSRARYEMASNPLYRKPISTHTVDFTFNKFNKSYNGTVD D7E4727CA310512B myo10_bovin Unconventional myosin-X MYO10 Unconventional myosin-10 protein protein bovin Bos taurus (Bovine) Bovine MDNFFPEGTRVWLRENGQHFPSTVNSCAEGVVVFQTDYGQVFTYKQSTITHQKVMPMQPTDEEGVDDMATLTELHGGAIMHNLYQRYKRNQIYTYIGSIIASVNPYKTITGLYSRDAVDRYSRCHLGELPPHVFAIANECYRCLWKRHDNQCVLISGESGAGKTESTKLILKFLSAISQQSVDLSSKEKTSSVEQAILESSPIMEAFGNAKTVYNNNSSRFGKFVQLNIGQKGNIQGGRIVDYLLEKNRVVRQNPGERNYHIFYALLAGLGHEEREEFYLSVPENYHYLNQSGCVTDRTISDQESFREVIMAMEVMQFSKEEVREVLRLLAGILHLGNIEFITAGGAQVSFKTALGRSAELLGLDPAQLTDALTQRSMFLRGEEILTPLNVQQAADSRDSLAMALYARCFEWVIKKINSRIKGKDDFKSIGILDIFGFENFEVNHFEQFNINYANEKLQEYFNKHIFSLEQLEYSREGLVWEDIDWIDNGECLDLIEKKLGLLALINEESHFPQATDSTLLEKLHNQHANNHFYVKPRVAVNNFGVKHYAGEVQYDVRGILEKNRDTFRDDLLNLLRESRFDFIYDLFEHVSSRNNQDTLKCGSKHRRPTVSSQFKDSLHSLMATLSASNPFFVRCIKPNMQKMPDQFDQAVVVNQLRYSGMLETVRIRKAGYAVRRPFQDFYKRYKVLMRNVALPEDIRGKCTALLQLYDASNSEWQLGKTKVFLRESLEQKLEKRQEEEVTRAAMVIRAHVLGYLARKQYKKVLDCVVIIQKNYRAFLLRRRFLHLKKAAVVFQKQLRGQIARRVYRQLLAEKRAEEEKRKREEEEKRKREEEERERERERREAELRAQQEEAARKQRELEALQQESQRAAELSRELEKQKENKQVEEILRLEKEIEDLQRMKERQELSLTEASLQKLQQLRDEELRRLEDEACRAAQEFLESLNFDEIDECVRNIERSLSVGSGCTGEQGAGAEKPSFNFSQPYPEEEEVDEGFEADDDAFKDSPNPSEHGHSDQRTSGIRTSDESSEEDPYMNDTVVPTSPSADSTVLLAPSEHDSSAGEPTYCLPQTPGALPAPEGDYDYDQDDYEDGAITSGSSVTFSNSCSSQWSPDYRCSVGTYNSSGAYRFSSEGAQSSFEDSEEDFDSRFDTDDELSYRRDSVYSCVTLPYFHSFLYMKGGLMNSWKRRWCVLKDETFLWFRSKQEALKQGWLHKKGGGSSTLSRRNWKKRWFVLRQAKLMYFENDSEEKLKGTVEVRAAKEIIDNTSKENGIDIIMADRTFHLIAESPEDASQWFSVLSQVHASTDQEIREMHDEQANPQNAVGTLDVGLIDSVCASDSPDRPNSFVIITANRVLHCNADTPEEMHHWITLLQRSKGDTRVEGQEFIVRGWLHKEVKNSPKMSSLKLKKRWFVLTHNSLDYYKSSEKNALKLGTLVLNSLCSVVPPDEKIFKETGYWNVTVYGRKHCYRLYTKLLNEATRWSSAIQNVTDTKAPIDTPTQQLIQDIKENCLNSDVVEQIYKRNPILRHTHHPLHSPLLPLPYGDINLNLLKDKGYTTLQDEAIKIFNSLQQLESMSDPIPIIQGILQTGHDLRPLRDELYCQLIKQTNKVPHPGSVGNLCSWQILTCLSCTFLPSRGILKYLKFHLRRIREQFPGTEMEKYALFIYESLKKTKCREFVPSRDEIEALIHRQEMTSTVHCHGGGSCKITVNSHTTAGEVVEKLIRGLAMEDSRNMFALFEYNGHVDKAIESRTIVADVLAKFEKLAATSEVGEQPWKFYFKLYCFLDTDNVPKDSVEFAFMFEQAHEAVIHGHYPAPEENLQVLAALRLQYLQGDYAPHAPVPPLEEVYSLQRLKARISQSTKSFTPGERLEKRRTSFLEGTLRRSFRTGSAIRQKAEEEQMVDMWVKEEVCSARASILDKWKKFQGMSQEQAMAKYMALIKEWPGYGSTLFDVECKEGGFPQDLWLGVSADAVSVYKRGEGRPLEVFQYEHILSFGAPLANTYKIVVDERELLFETSEVVDVAKLMKAYISMIVKKRYSTSRSVSSQGSSR DIP protein P79114 original sequence version: 64 DIP protein P79114 original sequence version: 87 43DF13424B4B2D28 itgb5-myo10-1 4 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Text reference (p 529). itgb5-myo10-3 3 10 unspecified role unspecified role prey prey myth4-ferm sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The region includes the MyTH4 and the FERM domain within the Myosin X tail and corresponds to aa [1551-2048] as inferred from uniprot. 14 unspecified role unspecified role bait bait P18084:p.Pro772Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:07:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro772ala' Tue Jun 30 18:56:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro772Ala' cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P18084:p.Tyr774Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 20:31:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr774ala' Tue Jun 30 18:29:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr774Ala' P18084:p.Leu773Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 19:18:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu773ala' Tue Jun 30 18:20:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu773Ala' 25 22 physical association physical association Fig 1a, S2. myo10-intb3-1 8 15 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association Fig 1d. intb3-myo10 6 15 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1f. intb3-itgav 6 13 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1e. myo10-itgb5 2 14 unspecified role unspecified role prey prey P18084:p.Pro772Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 19:54:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro772ala' Tue Jun 30 16:40:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro772Ala' cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 15 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ferm domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 41 45 direct interaction direct interaction Fig 2b. itgb1-myo10 5 15 unspecified role unspecified role prey prey ferm domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait physical association physical association Fig S1. itgb3-myo10-1 1 12 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig S3 (a, b). itgb1-myo10-1 7 15 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig S4. The colocalization of integrin beta-1 with myosin X at filopodial tips was enhanced in GFP-Myo10 transfected HeLa cells, but not in cells transfected with GFP-Myo10-HMM or GFP-Myo10-Tail.