IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol. Cell (1097-2765) 2003 imex curation Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast pull down pull down sequence tag sequence tag identification sequence tag Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Accepted 2012-JUN-22 by PPORRAS Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol. Cell (1097-2765) 2003 imex curation Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Accepted 2012-JUN-22 by PPORRAS Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol. Cell (1097-2765) 2003 imex curation Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Accepted 2012-JUN-22 by PPORRAS Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol. Cell (1097-2765) 2003 imex curation Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Accepted 2012-JUN-22 by PPORRAS Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation pex13_yeast Peroxisomal membrane protein PAS20 PEX13 PAS20 YLR191W L9470.1 Peroxin-13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSTAVPRPKPWETSASLEEPQRNAQSLSAMMTSNQQDSRPTEESNNSNSASESAPEVLPRPAALNSSGTYGESNTIPGIYGNSNYGIPYDNNPYSMNSIYGNSIGRYGYGGSYYGNNYGSFYGGGYGAGAGYGMNNGSGLGESTKATFQLIESLIGAVTGFAQMLESTYMATHNSFFTMISVAEQFGNLKEMLGSFFGIFAIMKFLKKILYRATKGRLGIPPKNFAESEGSKNKLIEDFQKFNDSGTINSNEKATRRKISWKPLLFFLMAVFGFPYLLNKFITKLQTSGTIRASQGNGSEPIDPSKLEFARALYDFVPENPEMEVALKKGDLMAILSKKDPLGRDSDWWKVRTKNGNIGYIPYNYIEIIKRRKKIEHVDDETRTH DBEA9A2372185860 DBEA9A2372185860 pex10_yeast Peroxisome biogenesis factor 10 PAS4 YDR265W D9954.8 PEX10 Peroxin-10 Peroxisome assembly protein 10 Peroxisomal biogenesis factor 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKNDNKLQKEALMRLSQLRFPFADAPSIVQAHQKDEQIQGLLIMKVTELCKLIKSQLFVNSYPKELSIFAKLLYLLFTTGRRGRTLGEEYVDLTYTNRKGTRLAGRLKMIVFAFAYPLCPYFITKLYKKIMKNNKESKIEDTESVAAFCKGLLDFILDVHMTLFYFKGAFYSISKRIFGMRYVFKHILSKNEANFREEGSQKYKVLGYILLAQNVMKWYPVLTSTLGSWIYGRKRTNDSITRSSVGLQERSEHESIEGIPKESQLTHINLSDKNQLPFIPEASRKCILCLMNMSDPSCAPCGHLFCWSCLMSWCKERPECPLCRQHCQPQEILVLRQ 9AF308A8AB2A0E28 pex14_yeast Peroxisomal membrane protein PEX14 PEX14 YGL153W G1870 Peroxin-14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTEKEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMATATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFRKKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDGMQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGMQEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP B714D7DA045E2DCB B714D7DA045E2DCB pex2_yeast Peroxisomal biogenesis factor 2 PEX2 CRT1 PAS5 YJL210W J0240 HRA271 Peroxin-2 Peroxisomal protein PAS5 Protein CRT1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRVAQLDSIALDKELYGQFWSEFNAAFNTSEHKEEWELALNTVVFMCATRFLPHYGSSCTYGSALSGVVFQCRKRTLYVVTVLAGYVWKKITHIIFNGPHCGNQMMWLKLYKWVNLLYHGCDVTNFLRFLAAEGPNARAFLSPLYRAFNVHSTRLIRDGSAIASEFYSNSVFAGLEYQNRQLLWNALLELFSNTLLTKRGLLTFVKKPPRSRSTTTYKTVCPRCGGFPTNPYQIACCRANYCYVCVVKALEWSMCDACGSSGRLTASPVY FAEFA3A526E82F3E FAEFA3A526E82F3E pex12_yeast Peroxisome assembly protein 12 PEX12 Peroxin-12 YM9711.16C YMR026C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFYSNLPSAGQSSRGSSTSGRNGVGLEPLYPTIFEIMSSQEIDSLLPASIRYLLANHLVANFPNRYTLRLNKYFFEWFQAIKGFVEWYHLKTYNSTFIDRFYGLQLFSSRDRNLALTQCLNPKGQSEWPQGLQLNQQQKSVIFLEKIILPYITAKLDEILEKISMNNIFSSDETENKWPKRAFLRIYPFIKKLLALSNLLVKLLFLTKRTGSVSLLQYLFKIEYTTVRPLSSELSGLKETKGMDNRLRKTNISSIFALMQGQLSIIPRFLTFMGSQFFPTFIFVLRVYQWWTTQDMTTKLQKRVNDLDEDIPRPPFSSHSDKTEDKEGVSEACPVCEKTVQNPCVLETGYVACYPCAISYLVNNEGHCPVTNKKLLGCTYNKHTNKWEVVTGIRKLLI 98E6300C2CBFA16A 98E6300C2CBFA16A pex15_yeast Peroxisomal membrane protein PEX15 Peroxisome biosynthesis protein PAS21 PEX15 Peroxin-15 PAS21 YOL044W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAASEIMNNLPMHSLDSSLRDLLNDDLFIESDESTKSVNDQRSEVFQECVNLFIKRDIKDCLEKMSEVGFIDITVFKSNPMILDLFVSACDIMPSFTKLGLTLQSEILNIFTLDTPQCIETRKIILGDLSKLLVINKFFRCCIKVIQFNLTDHTEQEEKTLELESIMSDFIFVYITKMRTTIDVVGLQELIEIFIFQVKVKLHHKKPSPNMYWALCKTLPKLSPTLKGLYLSKDVSIEDAILNSIDNKIQKDKAKSKGKQRGVKQKIHHFHEPMLHNSSEEQVKVEDAFNQRTSTDSRLQSTGTAPRKKNNDITVLAGSFWAVLKHHFTRSVLNKNGLLLTGLLLLLCLKKYKSLMAIFKHVPAAFHTVYPQIVGLLKLLASI B575A8D4B0DAC0B8 B575A8D4B0DAC0B8 pex8_yeast Peroxisomal biogenesis factor 8 YGR077C PEX8 PAS6 Peroxin-8 Peroxisomal protein PAS6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFDHDVEYLITALSSETRIQYDQRLLDEIAANVVYYVPRVKSPDTLYRLVGALFRSQFIVQLPPLRLLHIVKDVFLWKLEVSEPTLPISKFYLVWNAVFESHRATWNLSQLMVLDGVLVTYPSFKQLNNAYFIDESSNKTALYYRNWKLQLFSPIWAQLWNTAIVRANLSIQHCLLIALALLFNQSNRSALLHGVDVSWNLVTEKLLDLLEEYVHGIVQPMEIFSTDSVLSTNLNHLASCLTSSITRSNEATLVNSVRKLERICRYLSDTVASLKEQQLDFKFQNVFILIILALKELSAMNMTILPNHKDTFYSMICLSLFHVHVLTQKIGTVGFPSYDYVYDNLVTYFIVMDDLSKITTVLELMKRNNTKQDPNKLVFYINFLNKITNYYGCRIRLPFITEFIEPLLHFDVFFSGKTGNTLDIEIKESIHTLTITVLSIDSSYSSQVAQWQVSRILVYLKMSMDQFIAGKLSANQILLIFGHLSTQLPSLHNYNKHLLRDSLHETYIRIVNVKNPEKKNVLIECLIVQIAFINNPHHLIGWLNICLQLINTHNKKLLQQLWEMVSSLESSLAIDWWYTTVLSSQSSKL 7683417142F81701 pex17_yeast Peroxisomal membrane protein PEX17 PEX17 PAS9 YNL214W N1319 Peroxin-17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSINSFPRNIDWPSNIGIKKIEGTNPTVNAIKGLLYNGGSIYAFLYFVIAMFVEPTLQKQYQQRNDFSLFVLLRLRRIIAQLQKRLVMTPVSSLGFNEQNNFVERSTQTSDDNIIREDNSHWAEMIYQLQNMKQELQYFNRSSGQPSESIDDFVFQIKMVTDQVELTDRSRAFSNKSRNIIQGIREIKGWFVNGQVPR A0D3641B23C63C4D pex5_yeast Peroxisomal targeting signal receptor PEX5 PAS10 YDR244W YD8419.11 Peroxisomal protein PAS10 Peroxin-5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDVGSCSVGNNPLAQLHKHTQQNKSLQFNQKNNGRLNESPLQGTNKPGISEAFISNVNAISQENMANMQRFINGEPLIDDKRRMEIGPSSGRLPPFSNVHSLQTSANPTQIKGVNDISHWSQEFQGSNSIQNRNADTGNSEKAWQRGSTTASSRFQYPNTMMNNYAYASMNSLSGSRLQSPAFMNQQQSGRSKEGVNEQEQQPWTDQFEKLEKEVSENLDINDEIEKEENVSEVEQNKPETVEKEEGVYGDQYQSDFQEVWDSIHKDAEEVLPSELVNDDLNLGEDYLKYLGGRVNGNIEYAFQSNNEYFNNPNAYKIGCLLMENGAKLSEAALAFEAAVKEKPDHVDAWLRLGLVQTQNEKELNGISALEECLKLDPKNLEAMKTLAISYINEGYDMSAFTMLDKWAETKYPEIWSRIKQQDDKFQKEKGFTHIDMNAHITKQFLQLANNLSTIDPEIQLCLGLLFYTKDDFDKTIDCFESALRVNPNDELMWNRLGASLANSNRSEEAIQAYHRALQLKPSFVRARYNLAVSSMNIGCFKEAAGYLLSVLSMHEVNTNNKKGDVGSLLNTYNDTVIETLKRVFIAMNRDDLLQEVKPGMDLKRFKGEFSF 553251971E0BFD8D pex14-pex12 4 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1A Membrane extraction with Digitonin pex14-pex15-1 4 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey association association Fig. 1A Membrane extraction using n-dodecyl-beta-D-maltopyranosid (DDM.) pex14-pex17-2 4 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey physical association physical association Fig. 1A Membrane extraction using CYMAL-4 pex14-pex10 4 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Fig. 1C Membrane extraction with 1% Digitonin pex2-pex10 4 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1C Membrane extraction with 1% Digitonin pex14-pex10-2 1 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Figs. 1A, 1B, 5A Membrane extraction with 1% Digitonin In one experiment, Prohibitin was also observed to be present but could not be ascribed to a single gene product. The presence of PEX17 was confirmed by immunostaining. pex2-pex10-1 1 13 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Figs. 1C Membrane extraction with 1% Digitonin. Presnce of PEX17 confirmed by western blot. pex14-pex17-3 2 12 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 2A, 4B Observed in wild type and PEX8-/- cells pex10-pex12 2 9 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 2A pex10-pex12-1 2 9 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 2A pex14-pex17-4 3 12 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 2B, 4B Observed in wild-type and PEX8-/- cells pex10-pex12-6 3 9 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Figs. 2B, 4C Observed in wild-type and PEX8-/- cells. pex2-pex10-2 4 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 3A Membrane extraction with 1% Digitonin. Observed in wild-type, PEX1-/- and PEX4-/-cells. pex2-pex10-3 4 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3A Membrane extraction with 1% Digitonin. Observed in Pex5-/- cells pex2-pex10-4 4 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 3A Membrane extraction with 1% Digitonin. Observed in Pex14-/- cells pex2-pex10-5 4 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey association association Fig. 3A Membrane extraction with 1% Digitonin. Observed in Pex13-/- cells. pex2-pex10-6 4 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Fig. 3A Membrane extraction with 1% Digitonin. Observed in PEX17-/-cells. pex10-pex2 4 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Fig. 3B Membrane extraction with 1% Digitonin. Observed in wild-type, PEX1-/- and PEX4-/-cells. pex10-pex12-3 4 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3B Membrane extraction with 1% Digitonin. Observed in PEX5-/-cells. pex10-pex12-4 4 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 3B Membrane extraction with 1% Digitonin. Observed in PEX17-/-cells. pex10-pex12-5 4 6 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association Fig. 3B Membrane extraction with 1% Digitonin. Observed in PEX8-/- and PEX13-/- cells. pex12-pex10 4 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey association association Fig. 3C Membrane extraction with 1% Digitonin. Observed in wild-type, PEX1-/- and PEX4-/-cells. pex12-pex10-1 4 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX5-/-cells. pex12-pex10-2 4 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX17-/-cells. pex12-pex10-3 4 9 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX14-/-cells. pex12-pex10-4 4 9 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey association association Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX8-/- and PEX13-/-cells. pex14-pex10-3 4 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 4A Membrane extraction with Digitonin pex14-pex10-7 4 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Fig. 1A Membrane extraction with Digitonin pex14-pex10-6 4 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 4A Membrane extraction with Digitonin. Observed in PEX8-/- cells pex2-pex10-10 4 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey association association Fig. 4A Membrane extraction with 1% Digitonin. pex2-pex10-9 4 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4A Membrane extraction with 1% Digitonin. Observed in PEX8-/- cells. pex8-pex10-1 1 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey association association Fig. 5A Membrane extraction with 1% Digitonin. Presence of PEX17 confrimed by western blot. pex8-pex10-2 4 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey association association Fig. 5D Membrane extraction with 1% Digitonin. Observed in wild-type cells. pex8-pex10-3 4 11 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Fig. 5D Membrane extraction with 1% Digitonin. Observed in PEX17-/- cells. pex8-pex10-4 4 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5D Membrane extraction with 1% Digitonin. Observed in PEX2-/-, PEX10-/- and PEX12-/- cells. pex2-pex10-2 1 8 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association Figs. 5A,5B Membrane extraction with 1% Digitonin. Presnce of PEX17 confirmed by western blot.