UniProt UniProt http://www.uniprot.org ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. J. Biol. Chem. (0021-9258) 2003 imex curation Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. Rat brain MATCHMAKER cDNA library (Clontech). Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de Accepted 2012-JUN-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Rat brain MATCHMAKER cDNA library (Clontech). J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. J. Biol. Chem. (0021-9258) 2003 imex curation Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. Rat brain MATCHMAKER cDNA library (Clontech). Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de Accepted 2012-JUN-08 by PPORRAS Rat brain MATCHMAKER cDNA library (Clontech). J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. J. Biol. Chem. (0021-9258) 2003 imex curation Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. Rat brain MATCHMAKER cDNA library (Clontech). Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de Accepted 2012-JUN-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Rat brain MATCHMAKER cDNA library (Clontech). J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation oprm_rat Mu-type opioid receptor Oprm1 Ror-b Opioid receptor B protein protein rat Rattus norvegicus (Rat) Rat MDSSTGPGNTSDCSDPLAQASCSPAPGSWLNLSHVDGNQSDPCGLNRTGLGGNDSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLP 9C916DE7C1C33743 arfg1_human ADP-ribosylation factor GTPase-activating protein 1 ADP-ribosylation factor 1 GTPase-activating protein ARF1-directed GTPase-activating protein ARFGAP1 ARF1GAP protein protein human Homo sapiens Human MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW CAE41828DE660621 pld2_rat Phospholipase D2 Pld2 Choline phosphatase 2 PLD1C Phosphatidylcholine-hydrolyzing phospholipase D2 protein protein rat Rattus norvegicus (Rat) Rat MTVTQTDLFPYGDYLNSSQLHMEPDVVDTLKEGEDPADRMHPFLAIYDLQPLRAHPLVFAPGVPVIAQVVGTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLNLARFAAAHSPAREAANENIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIRKRSGGHRVPGFTCCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTEARYGVRIDTSHRSLILKCSSYRQARWWGQEITELAQGPGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAREEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTLMLLHPNIKVMRHPDLVTLWAHHEKLLVVDQAVAFLGGLDLAYGRWDDVQYRLTDLGDPSESADSQTPTPGSDPAATPDLSHNHFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFIQRWNFTKTIKARYKIPQYPYLLPKSASTANHLPFIIPGGQCATVQVLRSVDRWSAGTLESSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKLLIADDRTVIIGSANINDRSLLGKRDSELAILIKDTEMEPSLMDGVEYQAGRFALSLRGRCFSVILGANTWPDLDLRDPVCDDFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHIRGHLVHFPLKFLEDESLLPHWGAKRGMIPLEVWT D430843B4D541EEA oprm1-pld2 2 4 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 9 physical association physical association no figure oprm1-pld2-1 3 4 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure oprm1-arfgap1 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 2a DAMGO; increases association of ARFGAP1/ARF1