mbinfo MBInfo http://www.mechanobio.info/ Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature (0028-0836) 2002 imex curation Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Accepted 2012-JUL-04 by ORCHARD Nature (0028-0836) 2002 Only protein-protein interactions imex curation Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature (0028-0836) 2002 imex curation Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Only protein-protein interactions bovin-brain Bos taurus brain brain Brain [cerebrum] chromatography chromatography technology column chromatography chromatography de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Accepted 2012-JUL-04 by ORCHARD Nature (0028-0836) 2002 Only protein-protein interactions imex curation Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature (0028-0836) 2002 imex curation Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Only protein-protein interactions bovin-brain Bos taurus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Accepted 2012-JUL-04 by ORCHARD Nature (0028-0836) 2002 Only protein-protein interactions imex curation f1mpz9_bovin NCKAP1 protein protein bovin Bos taurus (Bovine) Bovine MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLSDHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA 5207B38542646176 wasf1_bovin Wiskott-Aldrich syndrome protein family member 1 WASF1 Protein WAVE-1 protein protein bovin Bos taurus (Bovine) Bovine MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPTTALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVTPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE E1CE0954B42C9C79 f1mx60_bovin Cytoplasmic FMR1-interacting protein CYFIP2 protein protein bovin Bos taurus (Bovine) Bovine MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGHEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMKFMYFQRKAIERFCSEVKRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNRITQCLHQQLEVIPGYEELLADIVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSNIYKLDAKKRINLSKIDKFFKQLQVVPLFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMVQIRDDHIRFISELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTDKFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLKGEKDPKGGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFFFTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMMEYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSVLLDKRFRAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQRISAAMYKSLDQAISRFESEDLTSIVELEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITLHVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPYYLYGSKPLNIAYSHIYSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIVKSLLQGTILQYVKTLIEVMPKICRLPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFCLLIEQALSQEEVCDLLHAAPFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSYLQDPVWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIPVGTNEFTAEQCFGDGLNWAGCSIIVLLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPLKKMADRIRKYQILNNEVFAILNKYMKSVETDSSTVEHVRCFQPPIHQSLATTC 413CC4F480B65696 a8yxx8_bovin BRK1 C22H3ORF10 protein protein bovin Bos taurus (Bovine) Bovine MAGQEDPVQREIHQDWANREYIEVITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTKGETLT 637B5476A9B4C457 rac1_human Ras-related C3 botulinum toxin substrate 1 RAC1 MIG5 p21-Rac1 Ras-like protein TC25 Cell migration-inducing gene 5 protein TC25 protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL ACEDF83A45E5EA67 c22h3orf10-cyfip2 2 7 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component association association Fig 1a (left). wasf1-c22h3orf10-1 3 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Fig 1a (Right). rac1-cyfip2-1 1 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] 4 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] association association Fig 3d. The Wave complex subunits (PIR121 and NAP125) bound tightly to GTP-bound Rac1 whereas the interaction was very weak with GDP-bound Rac1.