mbinfo MBInfo http://www.mechanobio.info/ WIP regulates N-WASP-mediated actin polymerization and filopodium formation. WIP regulates N-WASP-mediated actin polymerization and filopodium formation. Nat. Cell Biol. (1465-7392) 2001 imex curation Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. Could not curate interactions from fig 1b, 2 and 3b since the source of recombinant WIP and GST-CDC42 could not be traced. Interactions involving F-actin could not be curated since the number of actin monomers in the filament is not definite. Interaction detection method: GST pull down. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Accepted 2012-JUN-25 by ORCHARD Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation WIP regulates N-WASP-mediated actin polymerization and filopodium formation. WIP regulates N-WASP-mediated actin polymerization and filopodium formation. Nat. Cell Biol. (1465-7392) 2001 imex curation Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Accepted 2012-JUN-25 by ORCHARD Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation WIP regulates N-WASP-mediated actin polymerization and filopodium formation. WIP regulates N-WASP-mediated actin polymerization and filopodium formation. Nat. Cell Biol. (1465-7392) 2001 imex curation Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Accepted 2012-JUN-25 by ORCHARD Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation WIP regulates N-WASP-mediated actin polymerization and filopodium formation. WIP regulates N-WASP-mediated actin polymerization and filopodium formation. Nat. Cell Biol. (1465-7392) 2001 imex curation Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Accepted 2012-JUN-25 by ORCHARD Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 wipf1_human WAS/WASL-interacting protein family member 1 WIPF1 WIP Wiskott-Aldrich syndrome protein-interacting protein Protein PRPL-2 WASPIP protein protein human Homo sapiens Human MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSPHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPPIPR DIP protein O43516 original sequence version: 128 DIP protein O43516 original sequence version: 97 DIP protein O43516 original sequence version: 131 09B7A95E6808A46D wasl_mouse Neural Wiskott-Aldrich syndrome protein Wasl protein protein mouse Mus musculus Mouse MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWAKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPAPSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED DIP protein Q91YD9 original sequence version: 57 F5ABF44DF9A9F716 wipf1_mouse WAS/WASL-interacting protein family member 1 Wiskott-Aldrich syndrome protein-interacting protein Wipf1 Wip Waspip protein protein mouse Mus musculus Mouse MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGASAGGYGGGGGGGGGGGGGGGGSGGNFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPILPPGGRATSAKPFSPPSGPGRFPAPSPGHRSGPPEPPRNRMPPPRPDVGSKPDSLPPPVPNTPRPVPSSLHNRGSPAGLGAPRPPFPGNRGAAFGAGSARQNPSGSSSPFPRPPLPPTPSRALDDKPPPPPPPVGNRPSMHREAVPPPPSQTSKPPVPSTPRPGLGSQAPPPPPPPSRPGPPPLPPASNDEIPRLPQRNLSLTSSAPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPINRNGSTARALPATPQLPSRSGMDSPRSGPRPPLPPDRPGAGAPPPPPPSTSVRNGFQDSSCEDEWESRFYFHPISDLPPPEPYVPTTKTYPSKLARNESRSGSNRRERGAPPLPPIPR DIP protein Q8K1I7 original sequence version: 101 0BADBE69463B5960 wasl_human Neural Wiskott-Aldrich syndrome protein WASL protein protein human Homo sapiens Human MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED DIP protein O00401 original sequence version: 55 DIP protein O00401 original sequence version: 147 DIP protein O00401 original sequence version: 150 DIP protein O00401 original sequence version: 136 DIP protein O00401 original sequence version: 138 DIP protein O00401 original sequence version: 125 DIP protein O00401 original sequence version: 112 DIP protein O00401 original sequence version: 126 B5895C6055E23049 q2ydc4_human WIPF1 protein protein human Homo sapiens Human MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGMTRRGVSCFQNPACPNVPIILSVKIV 97A90B3546A46143 wasl_rat Neural Wiskott-Aldrich syndrome protein Wasl protein protein rat Rattus norvegicus (Rat) Rat MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYSSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPLSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPASSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFQDDDEWED DIP protein O08816 original sequence version: 145 DIP protein O08816 original sequence version: 115 480E21F26F7FC77E wipf1-wasl-1 2 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig 1a. wasl-wipf1-1 3 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This region includes the WH1 domain of N-WASP. physical association physical association Fig 1d. wipf1-acta1 1 5 unspecified role unspecified role prey prey rabit Oryctolagus cuniculus (Rabbit) Rabbit 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This region contains the verprolin homology region [1-117] of human WIPF1. ecoli Escherichia coli (strain K12) physical association physical association Fig 3a. wipf1-wasl-3 4 11 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 10 unspecified role unspecified role bait bait region uncategorized protein modification uncategorized protein modification undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Human WIPF1 protein tagged with an Xpress tag. region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Fig 1c. wasl-wipf1 3 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1e.