mbinfo MBInfo http://www.mechanobio.info/ Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. J. Cell Biol. (0021-9525) 2000 imex curation Nikolopoulos S.N., Turner C.E. Could not curate interactions from Fig 4C, 5, 6, 7A, 8 and 9 since the source of recombinant actopaxin proteins (GST-actopaxin, GFP-actopaxin, Xpress-tagged actopaxin) was not specified in the paper and could not be traced. interaction detection method: GST pull down. The source of actopaxin inferred from the cell lysates (REF-52) used and the source of paxillin inferred from the amino acid (559) length specified by the author. turnerc@mail.upstate.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-intestinal muscle Human intestinal smooth mucle (HISM) intestinal_muscle intestinal muscle anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nikolopoulos S.N., Turner C.E. Could not curate interactions from Fig 4C, 5, 6, 7A, 8 and 9 since the source of recombinant actopaxin proteins (GST-actopaxin, GFP-actopaxin, Xpress-tagged actopaxin) was not specified in the paper and could not be traced. interaction detection method: GST pull down. The source of actopaxin inferred from the cell lysates (REF-52) used and the source of paxillin inferred from the amino acid (559) length specified by the author. turnerc@mail.upstate.edu Accepted 2012-MAY-29 by ORCHARD 20-Jun-2012: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2000 Only protein-protein interactions imex curation Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. J. Cell Biol. (0021-9525) 2000 imex curation Nikolopoulos S.N., Turner C.E. Could not curate interactions from Fig 4C, 5, 6, 7A, 8 and 9 since the source of recombinant actopaxin proteins (GST-actopaxin, GFP-actopaxin, Xpress-tagged actopaxin) was not specified in the paper and could not be traced. interaction detection method: GST pull down. The source of actopaxin inferred from the cell lysates (REF-52) used and the source of paxillin inferred from the amino acid (559) length specified by the author. turnerc@mail.upstate.edu 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nikolopoulos S.N., Turner C.E. Could not curate interactions from Fig 4C, 5, 6, 7A, 8 and 9 since the source of recombinant actopaxin proteins (GST-actopaxin, GFP-actopaxin, Xpress-tagged actopaxin) was not specified in the paper and could not be traced. interaction detection method: GST pull down. The source of actopaxin inferred from the cell lysates (REF-52) used and the source of paxillin inferred from the amino acid (559) length specified by the author. turnerc@mail.upstate.edu Accepted 2012-MAY-29 by ORCHARD J. Cell Biol. (0021-9525) 2000 Only protein-protein interactions imex curation paxi_chick Paxillin PXN protein protein chick Gallus gallus (Chicken) Chicken MDDLDALLADLESTTSHISKRPVFLTEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVIDPLDQWQPSVSRYGHQQPQSQSPIYSSSAKSSSASVPRDGLSSPSPRASEEEHVYSFPNKQKSAEPSPTMTSTSLGSNLSELDRLLLELNAVQHNPPSGFSADEVSRSPSLPNVTGPHYVIPESSSSAGGKAAPPTKEKPKRNGGRGIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQRVNASQQQTRISASSATRELDELMASLSDFKFMAQGKAGGSSSPPSTTPKPGSQLDTMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGVFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFINGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMGKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC 6450270D90B2DE84 parva_human Alpha-parvin Calponin-like integrin-linked kinase-binding protein CH-ILKBP Matrix-remodeling-associated protein 2 Actopaxin PARVA MXRA2 protein protein human Homo sapiens Human MATSPQKSPSVPKSPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSELQEEGMNAINLPLSPIPFELDPEDTMLEENEVRTMVDPNSRSDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLESEKLNVAEVTQSEIAQKQKLQTVLEKINETLKLPPRSIKWNVDSVHAKSLVAILHLLVALSQYFRAPIRLPDHVSIQVVVVQKREGILQSRQIQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEGYFVPLHSFFLTPDSFEQKVLNVSFAFELMQDGGLEKPKPRPEDIVNCDLKSTLRVLYNLFTKYRNVE DIP protein Q9NVD7 original sequence version: null F48BB5B1E83F8CEF paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC DIP protein P49023 original sequence version: 115 DIP protein P49023 original sequence version: 126 DIP protein P49023 original sequence version: 169 DIP protein P49023 original sequence version: 140 DIP protein P49023 original sequence version: 175 ABF6C0BE5939623F parva_rat Alpha-parvin Parva Actp Actopaxin protein protein rat Rattus norvegicus (Rat) Rat MATSPQKSPSVPKSPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSEFQEEGMNAINLPLSPISFELDPEDTMLEENEVRTMVDPNSRNDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLESEKLNVAEVTQSEIAQKQKLQTVLEKINEALKLPPRSIKWNVDSVHAKNLVAILHLLVALSQYFRAPIRLPDHVSIQVVVVQKREGILQSRQIQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEGYFVPLHSFFLTPDSFEQKVLNVSFAFELMQDGGLEKPKPRPEDIVNCDLKSTLRVLYNLFTKYRNVE 7AAD24EBC25D094C pxn-parva-1 2 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ld1 motif sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined The sequence range for the paxillin LD1 motif was not given by the author and also could not be found in the chick paxillin swissprot entry. ld4 motif sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined The sequence range for paxillin LD4 motif was not given by the author and also could not be found on the chick paxillin swisprot entry page. ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey rat-embryonic fibroblast Rat embryonic fibroblast embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association Fig 4B (top lane). actopaxin expressed in REF-52 cells. parva-pxn-1 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig 7B.