mbinfo MBInfo http://www.mechanobio.info/ IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature (0028-0836) 2000 imex curation Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Experiments showing interactions with purified IRSp53 (GST-IRSp53) in fig 1c, 1d could not be curated since the amino acid length (521) specified by the author could be matched to two organisms (bovine and Chinese hamster) and the specific one used here could not be identified. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp Accepted 2012-JUL-18 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Nature (0028-0836) 2000 Only protein-protein interactions imex curation IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature (0028-0836) 2000 imex curation Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp Accepted 2012-JUL-18 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Nature (0028-0836) 2000 Only protein-protein interactions imex curation IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature (0028-0836) 2000 imex curation Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Miki H., Yamaguchi H., Suetsugu S., Takenawa T. The source of IRSp53 inferred from cDNA screened in two-hybrid experiment and the source of WAVE2 traced from references. Experiments showing interactions with purified IRSp53 (GST-IRSp53) in fig 1c, 1d could not be curated since the amino acid length (521) specified by the author could be matched to two organisms (bovine and Chinese hamster) and the specific one used here could not be identified. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp Accepted 2012-JUL-18 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Nature (0028-0836) 2000 Only protein-protein interactions imex curation IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature (0028-0836) 2000 imex curation Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp Accepted 2012-JUL-18 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Nature (0028-0836) 2000 Only protein-protein interactions imex curation IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature (0028-0836) 2000 imex curation Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. Interaction detection method: two-hybrid screening. Interactions involving Rac1 and Cdc42 (Fig 3b, 3c, 3d and 4a) could not be curated since the source of these proteins could not be traced. The in vivo coimmunoprecipitation experiment in fig 1a could not be curated since the specific WAVE isoform coprecipitated was not mentioned. takenawa@ims.u-tokyo.ac.jp Accepted 2012-JUL-18 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. human brain cDNA library. Nature (0028-0836) 2000 Only protein-protein interactions imex curation fyn_human Tyrosine-protein kinase Fyn p59-Fyn Proto-oncogene c-Fyn Src-like kinase FYN Proto-oncogene Syn protein protein human Homo sapiens Human MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL DIP protein P06241 original sequence version: 195 DIP protein P06241 original sequence version: 147 DIP protein P06241 original sequence version: 164 4A1E443A4B5A0977 srbs2_human Sorbin and SH3 domain-containing protein 2 SORBS2 ARGBP2 KIAA0777 Arg/Abl-interacting protein 2 Sorbin Arg-binding protein 2 protein protein human Homo sapiens Human MSYYQRPFSPSAYSLPASLNSSIVMQHGTSLDSTDTYPQHAQSLDGTTSSSIPLYRSSEEEKRVTVIKAPHYPGIGPVDESGIPTAIRTTVDRPKDWYKTMFKQIHMVHKPDDDTDMYNTPYTYNAGLYNPPYSAQSHPAAKTQTYRPLSKSHSDNSPNAFKDASSPVPPPHVPPPVPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPRSIFEYEPGKSSILQHERPASLYQSSIDRSLERPMSSASMASDFRKRRKSEPAVGPPRGLGDQSASRTSPGRVDLPGSSTTLTKSFTSSSPSSPSRAKGGDDSKICPSLCSYSGLNGNPSSELDYCSTYRQHLDVPRDSPRAISFKNGWQMARQNAEIWSSTEETVSPKIKSRSCDDLLNDDCDSFPDPKVKSESMGSLLCEEDSKESCPMAWGSPYVPEVRSNGRSRIRHRSARNAPGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYESEQQHKDLLRAWSQCSTEEVPRDMVPTRISEFEKLIQKSKSMPNLGDDMLSPVTLEPPQNGLCPKRRFSIEYLLEEENQSGPPARGRRGCQSNALVPIHIEVTSDEQPRAHVEFSDSDQDGVVSDHSDYIHLEGSSFCSESDFDHFSFTSSESFYGSSHHHHHHHHHHHRHLISSCKGRCPASYTRFTTMLKHERARHENTEEPRRQEMDPGLSKLAFLVSPVPFRRKKNSAPKKQTEKAKCKASVFEALDSALKDICDQIKAEKKRGSLPDNSILHRLISELLPDVPERNSSLRALRRSPLHQPLHPLPPDGAIHCPPYQNDCGRMPRSASFQDVDTANSSCHHQDRGGALQDRESPRSYSSTLTDMGRSAPRERRGTPEKEKLPAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPEKAQPARPPPPAQPGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKIPGTNRQGIFPVSYVEVVKKNTKGAEDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALYNYTPRNEDELELRESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL 7E98B196D4DB38E6 wasf2_human Wiskott-Aldrich syndrome protein family member 2 WASF2 WAVE2 Protein WAVE-2 Verprolin homology domain-containing protein 2 protein protein human Homo sapiens Human MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD DIP protein Q9Y6W5 original sequence version: 93 DIP protein Q9Y6W5 original sequence version: 121 DIP protein Q9Y6W5 original sequence version: 137 C737CE963016DE94 baip2_human Brain-specific angiogenesis inhibitor 1-associated protein 2 Insulin receptor substrate protein of 53 kDa Insulin receptor substrate p53/p58 Fas ligand-associated factor 3 BAIAP2 protein protein human Homo sapiens Human MSLSRSEEMHRLTENVYKTIMEQFNPSLRNFIAMGKNYEKALAGVTYAAKGYFDALVKMGELASESQGSKELGDVLFQMAEVHRQIQNQLEEMLKSFHNELLTQLEQKVELDSRYLSAALKKYQTEQRSKGDALDKCQAELKKLRKKSQGSKNPQKYSDKELQYIDAISNKQGELENYVSDGYKTALTEERRRFCFLVEKQCAVAKNSAAYHSKGKELLAQKLPLWQQACADPSKIPERAVQLMQQVASNGATLPSALSASKSNLVISDPIPGAKPLPVPPELAPFVGRMSAQESTPIMNGVTGPDGEDYSPWADRKAAQPKSLSPPQSQSKLSDSYSNTLPVRKSVTPKNSYATTENKTLPRSSSMAAGLERNGRMRVKAIFSHAAGDNSTLLSFKEGDLITLLVPEARDGWHYGESEKTKMRGWFPFSYTRVLDSDGSDRLHMSLQQGKSSSTGNLLDKDDLAIPPPDYGAASRAFPAQTASGFKQRPYSVAVPAFSQGLDDYGARSMSRNPFAHVQLKPTVTNDRCDLSAQGPEGREHGDGSARTLAGR DIP protein Q9UQB8 original sequence version: 87 3B9EDC6405DCC99D wasf1_human Wiskott-Aldrich syndrome protein family member 1 WASF1 KIAA0269 SCAR1 WAVE1 Protein WAVE-1 Verprolin homology domain-containing protein 1 protein protein human Homo sapiens Human MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE DIP protein Q92558 original sequence version: 78 DIP protein Q92558 original sequence version: 82 DIP protein Q92558 original sequence version: 124 DIP protein Q92558 original sequence version: 87 44B4527BDB77BC6E wasl_mouse Neural Wiskott-Aldrich syndrome protein Wasl protein protein mouse Mus musculus Mouse MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWAKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPAPSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED DIP protein Q91YD9 original sequence version: 57 F5ABF44DF9A9F716 q9uqb8-4 Brain-specific angiogenesis inhibitor 1-associated protein 2 BAIAP2-alpha BAIAP2 Fas ligand-associated factor 3 Insulin receptor substrate p53/p58 Insulin receptor substrate protein of 53 kDa protein protein human Homo sapiens Human MSLSRSEEMHRLTENVYKTIMEQFNPSLRNFIAMGKNYEKALAGVTYAAKGYFDALVKMGELASESQGSKELGDVLFQMAEVHRQIQNQLEEMLKSFHNELLTQLEQKVELDSRYLSAALKKYQTEQRSKGDALDKCQAELKKLRKKSQGSKNPQKYSDKELQYIDAISNKQGELENYVSDGYKTALTEERRRFCFLVEKQCAVAKNSAAYHSKGKELLAQKLPLWQQACADPSKIPERAVQLMQQVASNGATLPSALSASKSNLVISDPIPGAKPLPVPPELAPFVGRMSAQESTPIMNGVTGPDGEDYSPWADRKAAQPKSLSPPQSQSKLSDSYSNTLPVRKSVTPKNSYATTENKTLPRSSSMAAGLERNGRMRVKAIFSHAAGDNSTLLSFKEGDLITLLVPEARDGWHYGESEKTKMRGWFPFSYTRVLDSDGSDRLHMSLQQGKSSSTGNLLDKDDLAIPPPDYGAASRAFPAQTASGFKQRPYSVAVPAFSQGLDDYGARSMSSGSGTLVSTV DIP protein Q9UQB8-4 original sequence version: 97 DIP protein Q9UQB8-4 original sequence version: 125 618A09FDBEB3A5C3 wasf1-baiap2-1 2 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait proline-rich region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association wasf1-sorbs2-1 2 10 unspecified role unspecified role bait bait proline-rich region sufficient to bind sufficient binding region sufficient to bind range range range range 7 unspecified role unspecified role prey prey physical association physical association wasl-fyn 4 11 unspecified role unspecified role prey prey mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells 6 unspecified role unspecified role bait bait sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig 1b. baiap2-wasf2-1 1 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells physical association physical association Fig 1c. wasf2-baiap2 4 12 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 unspecified role unspecified role bait bait proline-rich region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig 1d, Suppl fig 2. baiap2-wasf2-2 3 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2a. baiap2-wasf2-4 4 8 unspecified role unspecified role prey prey proline-rich region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 49 45 physical association physical association Fig 2c (top). baiap2-wasf2-6 5 8 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 unspecified role unspecified role bait bait sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Suppl. fig 1b.