IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the DNA binding domain of E. coli SSB bound to ssDNA. Structure of the DNA binding domain of E. coli SSB bound to ssDNA. Nat. Struct. Biol. (1072-8368) 2000 imex curation Raghunathan S., Kozlov A.G., Lohman T.M., Waksman G. multiwavelength anomalous dispersion (MAD) phasing method lohman@ biochem.wustl.edu, waksman@biochem.wustl.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Raghunathan S., Kozlov A.G., Lohman T.M., Waksman G. multiwavelength anomalous dispersion (MAD) phasing method lohman@ biochem.wustl.edu, waksman@biochem.wustl.edu Accepted 2013-JAN-15 by MGT 04-Feb-2013: Contacted by IntAct-Help. Nat. Struct. Biol. (1072-8368) 2000 Only protein-protein interactions imex curation ssdna_dc ssDNA_dC ss dna single stranded deoxyribonucleic acid ss DNA ss dna chemical synthesis Chemical synthesis (Chemical synthesis) CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC ssb_ecoli Single-stranded DNA-binding protein Helix-destabilizing protein ssb exrB lexC b4059 JW4020 protein protein ecoli Escherichia coli (strain K12) MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF DIP protein P0AGE0 original sequence version: 44 F0AF43B6DC8D02FC ssb-ssdna-1 1 2 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1, Table 1 R = 0.26 Rworking = 0.26 Rfree = 0.3 2.8A Residues 41-49 were missing and 113-135 were unordered and therefore only 1-116 are modelled in pdbe.