UniProt UniProt http://www.uniprot.org Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Biochem. Biophys. Res. Commun. (0006-291X) 2000 imex curation Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov 15-Nov-2012: Contacted by pporras. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov Accepted 2012-OCT-03 by ORCHARD 15-Nov-2012: Contacted by pporras. Biochem. Biophys. Res. Commun. (0006-291X) 2000 Only protein-protein interactions imex curation Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Biochem. Biophys. Res. Commun. (0006-291X) 2000 imex curation Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov 15-Nov-2012: Contacted by pporras. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov Accepted 2012-OCT-03 by ORCHARD 15-Nov-2012: Contacted by pporras. Biochem. Biophys. Res. Commun. (0006-291X) 2000 Only protein-protein interactions imex curation Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Biochem. Biophys. Res. Commun. (0006-291X) 2000 imex curation Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov 15-Nov-2012: Contacted by pporras. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov Accepted 2012-OCT-03 by ORCHARD 15-Nov-2012: Contacted by pporras. Biochem. Biophys. Res. Commun. (0006-291X) 2000 Only protein-protein interactions imex curation Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Biochem. Biophys. Res. Commun. (0006-291X) 2000 imex curation Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov 15-Nov-2012: Contacted by pporras. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Take H., Watanabe S., Takeda K., Yu Z.X., Iwata N., Kajigaya S. kajigays@nih.gov Accepted 2012-OCT-03 by ORCHARD 15-Nov-2012: Contacted by pporras. Biochem. Biophys. Res. Commun. (0006-291X) 2000 Only protein-protein interactions imex curation sh3k1_human SH3 domain-containing kinase-binding protein 1 SH3KBP1 CIN85 Cbl-interacting protein of 85 kDa Human Src family kinase-binding protein 1 CD2-binding protein 3 protein protein human Homo sapiens Human MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPPDFEKEGNRPKKPPPPSAPVIKQGAGTTERKHEIKKIPPERPEMLPNRTEEKERPEREPKLDLQKPSVPAIPPKKPRPPKTNSLSRPGALPPRRPERPVGPLTHTRGDSPKIDLAGSSLSGILDKDLSDRSNDIDLEGFDSVVSSTEKLSHPTTSRPKATGRRPPSQSLTSSSLSSPDIFDSPSPEEDKEEHISLAHRGVDASKKTSKTVTISQVSDNKASLPPKPGTMAAGGGGPAPLSSAAPSPLSSSLGTAGHRANSPSLFGTEGKPKMEPAASSQAAVEELRTQVRELRSIIETMKDQQKREIKQLLSELDEEKKIRLRLQMEVNDIKKALQSK 3BD350FCDB14BD4C cbl_human E3 ubiquitin-protein ligase CBL Signal transduction protein CBL Proto-oncogene c-Cbl Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 CBL CBL2 RNF55 RING-type E3 ubiquitin transferase CBL protein protein human Homo sapiens Human MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT DIP protein P22681 original sequence version: 173 DIP protein P22681 original sequence version: 128 DIP protein P22681 original sequence version: 145 DIP protein P22681 original sequence version: 150 DIP protein P22681 original sequence version: 191 DIP protein P22681 original sequence version: 91 DIP protein P22681 original sequence version: 139 1AA6BF67377322CA cbl-sh3kbp1 3 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association no figure cbl-sh3kbp1-1 4 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 3B and 4B cbl-sh3kbp1-3 1 6 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3C sh3kbp1-cbl 2 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection P22681:p.[Tyr700Phe;Tyr731Phe;Ile744Phe] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain I F Wed May 02 20:24:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr700phe,tyr731phe,ile744phe' Tue Jun 30 19:31:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr700Phe;Tyr731Phe;Ile744Phe]' P22681:p.[Tyr700Phe;Tyr731Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 17:13:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr700phe,tyr731phe' Tue Jun 30 16:01:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr700Phe;Tyr731Phe]' physical association physical association 5 and 6B EGF treatment c-CBL association is correlated with tyrosine phosphorylation