mbinfo MBInfo http://www.mechanobio.info/ The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell (0092-8674) 1999 imex curation Rohatgi R., Ma L., Miki H., Lopez M., Kirchhausen T., Takenawa T., Kirschner M.W. The interaction between G-actin and N-WASP could not be curated since the specific actin isoform that was used was not specified. Interaction detection method: GST pull down assay. The source of N-WASP and Arp2/3 complex specified by the author. marc@hms.harvard.edu Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rohatgi R., Ma L., Miki H., Lopez M., Kirchhausen T., Takenawa T., Kirschner M.W. The interaction between G-actin and N-WASP could not be curated since the specific actin isoform that was used was not specified. Interaction detection method: GST pull down assay. The source of N-WASP and Arp2/3 complex specified by the author. marc@hms.harvard.edu Accepted 2012-JUN-25 by ORCHARD Cell (0092-8674) 1999 Only protein-protein interactions imex curation wasl_bovin Neural Wiskott-Aldrich syndrome protein WASL protein protein bovin Bos taurus (Bovine) Bovine MSSGQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQINNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVGAPPPPPNRMYPPPLPALPSSAPSGPPPPPPPLSVSGSVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTPAGSKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVTDAPESTPPAPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDDEDFEDDDEWED 54B83B48F1CDB3B8 arp2_bovin Actin-related protein 2 ACTR2 ARP2 Actin-like protein 2 protein protein bovin Bos taurus (Bovine) Bovine MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR DIP protein A7MB62 original sequence version: 6 DIP protein A7MB62 original sequence version: 35 DIP protein A7MB62 original sequence version: 47 1BFA6B442ED1A797 actr2-actr3 1 3 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] 2 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cofilin homology and acidic domains sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) association association Fig 3B. The interaction was demonstrated between N-WASP and the Arp 2/3 complex (composed of Arp3, Arp2, p41-Arc, p34-Arc, p21-Arc, p20-Arc, p16-Arc).