MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. FEBS Lett. (0014-5793) 2012 imex curation Renault TT., Grandier-Vazeille X., Arokium H., Velours G., Camougrand N., Priault M., Teijido O., Dejean LM., Manon S. suggested by lperfetto Only protein-protein interactions mouse-fl5.12 murine FL5.12 pro-B-cell lymphoid cell line fl5.12 FL5.12 murine pro-B-cell lymphoid cell line, dependent on IL3 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Renault TT., Grandier-Vazeille X., Arokium H., Velours G., Camougrand N., Priault M., Teijido O., Dejean LM., Manon S. suggested by lperfetto FEBS Lett. (0014-5793) 2012 Only protein-protein interactions imex curation The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. FEBS Lett. (0014-5793) 2012 imex curation Renault TT., Grandier-Vazeille X., Arokium H., Velours G., Camougrand N., Priault M., Teijido O., Dejean LM., Manon S. suggested by lperfetto Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Renault TT., Grandier-Vazeille X., Arokium H., Velours G., Camougrand N., Priault M., Teijido O., Dejean LM., Manon S. suggested by lperfetto FEBS Lett. (0014-5793) 2012 Only protein-protein interactions imex curation The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. FEBS Lett. (0014-5793) 2012 imex curation Renault TT., Grandier-Vazeille X., Arokium H., Velours G., Camougrand N., Priault M., Teijido O., Dejean LM., Manon S. suggested by lperfetto Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page mudpit multidimensional protein identification technology mudpit Renault TT., Grandier-Vazeille X., Arokium H., Velours G., Camougrand N., Priault M., Teijido O., Dejean LM., Manon S. suggested by lperfetto FEBS Lett. (0014-5793) 2012 Only protein-protein interactions imex curation tom22_yeast Mitochondrial import receptor subunit TOM22 MOM22 TOM22 MAS17 MAS22 YNL131W N1217 N1862 Mitochondrial 22 kDa outer membrane protein Translocase of outer membrane 22 kDa subunit Protein MAS17 Mitochondrial 17 kDa assembly protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTLTTTALLLGVPLSLSILAEQQLIEMEKTFDLQSDANNILAQGEKDAAATAN 6ACEC5D37D143CD3 6ACEC5D37D143CD3 bax_mouse Apoptosis regulator BAX Bax protein protein mouse Mus musculus Mouse MDGSGEQLGSGGPTSSEQIMKTGAFLLQGFIQDRAGRMAGETPELTLEQPPQDASTKKLSECLRRIGDELDSNMELQRMIADVDTDSPREVFFRVAADMFADGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLVWIQDQGGWEGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG D2E0B3566579FAFF bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG DIP protein Q07812 original sequence version: 73 DIP protein Q07812 original sequence version: 156 DIP protein Q07812 original sequence version: 92 DIP protein Q07812 original sequence version: 94 DIP protein Q07812-1 original sequence version: 93 DIP protein Q07812 original sequence version: 95 DIP protein Q07812 original sequence version: 120 DIP protein Q07812 original sequence version: 126 DIP protein Q07812 original sequence version: 143 DIP protein Q07812 original sequence version: 172 DIP protein Q07812 original sequence version: 175 6C0CDB0A7DEE4994 tom70_yeast Mitochondrial import receptor subunit TOM70 TOM70 MAS70 YNL121C N1905 OMP1 70 kDa mitochondrial outer membrane protein Translocase of outer membrane 70 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MKSFITRNKTAILATVAATGTAIGAYYYYNQLQQQQQRGKKNTINKDEKKDTKDSQKETEGAKKSTAPSNPPIYPVSSNGEPDFSNKANFTAEEKDKYALALKDKGNQFFRNKKYDDAIKYYNWALELKEDPVFYSNLSACYVSVGDLKKVVEMSTKALELKPDYSKVLLRRASANEGLGKFADAMFDLSVLSLNGDFNDASIEPMLERNLNKQAMSKLKEKFGDIDTATATPTELSTQPAKERKDKQENLPSVTSMASFFGIFKPELTFANYDESNEADKELMNGLSNLYKRSPESYDKADESFTKAARLFEEQLDKNNEDEKLKEKLAISLEHTGIFKFLKNDPLGAHEDIKKAIELFPRVNSYIYMALIMADRNDSTEYYNYFDKALKLDSNNSSVYYHRGQMNFILQNYDQAGKDFDKAKELDPENIFPYIQLACLAYRENKFDDCETLFSEAKRKFPEAPEVPNFFAEILTDKNDFDKALKQYDLAIELENKLDGIYVGIAPLVGKATLLTRNPTVENFIEATNLLEKASKLDPRSEQAKIGLAQMKLQQEDIDEAITLFEESADLARTMEEKLQAITFAEAAKVQQRIRSDPVLAKKIQETLAKLREQGLM 664B8FC32CCE39A2 664B8FC32CCE39A2 tom40_yeast Mitochondrial import receptor subunit TOM40 TOM40 ISP42 YMR203W YM8325.04 MOM38 Mitochondrial import site protein ISP42 Translocase of outer membrane 40 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDTYKQLHSHRQSLELVNPGTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSHTFSIGSQALPKYAFSALFANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDGQPTMCQLEQDYQASDFSVNVKTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYSRTDGSAPGDAGVSYLTRYVSKKQDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQAGMVPITDPLMGTPIGIQPTVEGSTTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLPTLSVLFCGEIDHFKNDTKIGCGLQFETAGNQELLMLQQGLDADGNPLQALPQL 6436FBBA4020464B tom22_mouse Mitochondrial import receptor subunit TOM22 homolog Tomm22 Tom22 Translocase of outer membrane 22 kDa subunit homolog protein protein mouse Mus musculus Mouse MAAAVAAAGAGEPLSPEELLPKAEAEKAEEELEEDDDDELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPPLPGKM A5182A39B6EBBFDA bax-tom22 2 TOM22 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BAX 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association f1b mint bax-tom70 2 TOM70 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BAX 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association f1b homomint mint bax-tom40-1 2 TOM40 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BAX 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association f1b homomint mint bax-tom22-1 2 TOM40 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level TOM22 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BAX 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell TOM70 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association mint f3c f3d the fact that hTom22(1-82) did not change the interaction between Bax and Tom22p, Tom40p or Tom70p, further supported the view that the effect of the hTom22(1-82) did not result from a simple competiting effect with the TOM complex but had a direct effect on Bax conformation. tomm22-bax 1 TOM22 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BAX 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BAX 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell association association f1c mint bax-bax-11 3 BAX 6 mudpit multidimensional protein identification technology mudpit predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed BAX 6 mudpit multidimensional protein identification technology mudpit predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed direct interaction direct interaction f4 homomint mint In the first dimension (Fig.4, horizontal direction) BaxP8G/P13G was observed as a range of oligomers between 200 and 350kDa. In the presence of hTom22(1-82), this profile disappeared and was replaced by a streak below 200kDa, showing that oligomers formed in the presence of the peptide had a lower size. A second dimension was run by layering the first-dimension lane on top of a SDS-PAGE. These conditions preserve organized protein complexes, such as the oligomers of ATP synthase subunit 9. After this second dimension (Fig.4, vertical direction), BaxP8G/P13G expressed alone was mainly found at 21kDa, the size of Bax monomer, with only a minor band at 43kDa, the size of Bax dimers. In the presence of hTom22(1-82), monomeric Bax could not be detected, and Bax was fully dimeric. This suggests that hTom22(1-82) stimulated the formation of a dimer of Bax that could not further oligomerize