IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) Aurora A was detected by immunostaining. The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The authors have confirmed that the IPs shown in Figs 6A/B were carried out using HeLa cells. I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The authors confirm that Myc-Cdh1/Cdc20 were used in this Experiment, rather than HA-Cdh1/Cdc20; also that SIRT-2 and mutants have an "HA" tag and are Human in origin. I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. Cancer Cell (1535-6108) 2011 imex curation Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer I cannot tell if the Fig 5A/5B bottom IP, used anti-tagWB or WB for participant detection, so have left as "WB". (antibodies for both myc and cdh1/cdc20 are used in the paper.) The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Accepted 2011-DEC-08 by JYOTI 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation cdc23_human Cell division cycle protein 23 homolog CDC23 ANAPC8 Anaphase-promoting complex subunit 8 Cyclosome subunit 8 protein protein human Homo sapiens Human MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP DIP protein Q9UJX2 original sequence version: 103 DIP protein Q9UJX2 original sequence version: 131 DIP protein Q9UJX2 original sequence version: 135 DIP protein Q9UJX2 original sequence version: 145 DIP protein Q9UJX2 original sequence version: 154 358F2B8745DB9D32 q8ixj6-2 NAD-dependent protein deacetylase sirtuin-2 SIRT2 SIR2L SIR2L2 SIR2-like protein 2 Regulatory protein SIR2 homolog 2 protein protein human Homo sapiens Human MDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ FFED07DEF9E3416A cdc16_human Cell division cycle protein 16 homolog ANAPC6 CDC16 homolog Anaphase-promoting complex subunit 6 Cyclosome subunit 6 CDC16 protein protein human Homo sapiens Human MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHALRSRKLDKLYEACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSDWEMSQSSIKSSICLLRGKIYDALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLESLPLSKLCNEEQELLRFLFENKLKKYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNKANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEHDQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKTAEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHSLMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIISPPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST A9C4F24615DF17EB cdc27_human Cell division cycle protein 27 homolog CDC27 D0S1430E D17S978E CDC27 homolog H-NUC ANAPC3 Anaphase-promoting complex subunit 3 protein protein human Homo sapiens Human MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF DIP protein P30260 original sequence version: 141 DIP protein P30260 original sequence version: 111 DIP protein P30260 original sequence version: 117 DIP protein P30260 original sequence version: 135 DIP protein P30260 original sequence version: 148 DIP protein P30260 original sequence version: 153 DIP protein P30260 original sequence version: 157 DIP protein P30260 original sequence version: 162 DIP protein P30260 original sequence version: 171 DIP protein P30260 original sequence version: 118 E6C8F59C1EF1DCBA cdc20_mouse Cell division cycle protein 20 homolog p55CDC Cdc20 protein protein mouse Mus musculus Mouse MAQFVFESDLHSLLQLDAPIPNAPVARWQRKAKEATGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRFIPQRSASQMEVASFLLSKENQPEDRGTPTKKEHQKAWSLNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWNAGSGDILQLLQMEQPGDYISSVAWIKEGNYLAVGTSNAEVQLWDVQQQKRLRNMTSHSARVSSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNIVNVWPSGPGESGWAPLQTFTQHQGAVKAVAWCPWQSNILATGGGTSDRHIRIWNVCSGACLSAVDVHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTARVLGLTMSPDGATVASAAADETLRLWRCFEMDPALRREREKASVAKSSLIHQGIR 81E0781BA87BA79E sir2_mouse NAD-dependent protein deacetylase sirtuin-2 SIR2-like protein 2 Sirt2 Sir2l2 Regulatory protein SIR2 homolog 2 protein protein mouse Mus musculus Mouse MAEPDPSDPLETQAGKVQEAQDSDSDTEGGATGGEAEMDFLRNLFTQTLGLGSQKERLLDELTLEGVTRYMQSERCRKVICLVGAGISTSAGIPDFRSPSTGLYANLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFIRLLKEKGLLLRCYTQNIDTLERVAGLEPQDLVEAHGTFYTSHCVNTSCRKEYTMGWMKEKIFSEATPRCEQCQSVVKPDIVFFGENLPSRFFSCMQSDFSKVDLLIIMGTSLQVQPFASLISKAPLATPRLLINKEKTGQTDPFLGMMMGLGGGMDFDSKKAYRDVAWLGDCDQGCLALADLLGWKKELEDLVRREHANIDAQSGSQAPNPSTTISPGKSPPPAKEAARTKEKEEQQ 15F96635445A1BC0 aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 apc4_human Anaphase-promoting complex subunit 4 APC4 Cyclosome subunit 4 ANAPC4 protein protein human Homo sapiens Human MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDTRSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEKVRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS DIP protein Q9UJX5 original sequence version: 78 DIP protein Q9UJX5 original sequence version: 115 DIP protein Q9UJX5 original sequence version: 120 DIP protein Q9UJX5 original sequence version: 124 DIP protein Q9UJX5 original sequence version: 133 80362CC8D8B2063F fzr1_mouse Fizzy-related protein homolog Fzr1 Fyr Fzr Cdh1/Hct1 homolog protein protein mouse Mus musculus Mouse MDQDYERRLLRQIIIQNENTVPCVSEMRRTLTPANSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEHKGLFTYSLSSKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNADQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFTRIR B5DC86653D74D9A5 sir2_human NAD-dependent protein deacetylase sirtuin-2 SIR2-like protein 2 SIRT2 SIR2L SIR2L2 Regulatory protein SIR2 homolog 2 protein protein human Homo sapiens Human MAEPDPSHPLETQAGKVQEAQDSDSDSEGGAAGGEADMDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ DIP protein Q8IXJ6 original sequence version: 113 A392442A8F6316F1 cdc20_human Cell division cycle protein 20 homolog CDC20 p55CDC protein protein human Homo sapiens Human MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR DIP protein Q12834 original sequence version: 132 DIP protein Q12834 original sequence version: 74 DIP protein Q12834 original sequence version: 100 DIP protein Q12834 original sequence version: 118 DIP protein Q12834 original sequence version: 125 DIP protein Q12834 original sequence version: 114 DIP protein Q12834 original sequence version: 147 DIP protein Q12834 original sequence version: 162 FD5C967AF84089E8 cdc27_mouse Cell division cycle protein 27 homolog Cdc27 protein protein mouse Mus musculus Mouse MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTTLVSNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSTVISPDNVPLGPGTAILSKQVQNKPKTGRSLLGGPTALSPLTPSFGILPLETPSPGDGSYLQNYTNTPSVIDVAPTGAPTKKSVARMGQTGTKSVFSQSGNSREVTPVLVAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGLTQPSINDSLEITKLDSSIISEGKITTVTPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF 554D8B1136826DA3 apc1_human Anaphase-promoting complex subunit 1 ANAPC1 TSG24 Cyclosome subunit 1 Testis-specific gene 24 protein Mitotic checkpoint regulator protein protein human Homo sapiens Human MSNFYEERTTMIAARDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGEDVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSNEVEKCICILQSSCINMHSIEGKDYIASLPFQVANVWPTKYGLLFERSASSHEVPPGSPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNTDPSIVMTYDAVQNVHSVWTLRRVKSEEENVVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGVQRFNISSHNQSPKRHSISHSPNSNSNGSFLAPETEPIVPELCIDHLWTETITNIREKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNIPAKDAAPVEKIDTMLVLEGSGNLVLYTGVVRVGKVFIPGLPAPSLTMSNTMPRPSTPLDGVSTPKPLSKLLGSLDEVVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSIRDPVHNRVTLELSNGSMVRITIPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYNVHSAPGGPSYHSEWNLFVTCLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDDDWEYLLNSDYHQNVESHLLNRSLCLSPSEASQMKDEDFSQNLSLDSSTLLFTHIPAIFFVLHLVYEELKLNTLMGEGICSLVELLVQLARDLKLGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGFMHHPSFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYILGDESLVSDESSQYLTRITIAPQKLQVEQEENRFSFRHSTSVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNHEVMSLIWSEDLRVQDVRRLLQSAHPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLNSGNIDVPPNMTSWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLSAPNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRLGRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHLPPIGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM DIP protein Q9H1A4 original sequence version: 85 DIP protein Q9H1A4 original sequence version: 119 DIP protein Q9H1A4 original sequence version: 128 DIP protein Q9H1A4 original sequence version: 137 E08B1FE3FC28917E fzr1_human Fizzy-related protein homolog FYR FZR KIAA1242 FZR1 CDH1 Cdh1/Hct1 homolog CDC20-like protein 1 protein protein human Homo sapiens Human MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNAEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKVKWESVSVLNLFTRIR DIP protein Q9UM11 original sequence version: 100 DIP protein Q9UM11 original sequence version: 131 DIP protein Q9UM11 original sequence version: 136 DIP protein Q9UM11 original sequence version: 145 F6C8FDAF81D8103A sirt2-aurka 6 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3B aurka-sirt2 1 16 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3B sirt2-anapc1 2 22 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey association association Fig 4A sirt2-anapc1-2 5 23 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey association association Fig 4B sirt2-fzr1 4 18 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Fig 4C fzr1-sirt2 4 15 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Fig 4D sirt2-cdc20 4 15 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Fig 4E cdc20-sirt2 4 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association Fig 4F fzr1-cdc27-6 5 19 unspecified role unspecified role ancillary ancillary region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 19:17:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187tyr' Tue Jun 30 19:12:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Tyr' 23 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role prey prey association association Fig 5A (top) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. cdc27-fzr1-1 9 19 unspecified role unspecified role ancillary ancillary Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 18:44:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187tyr' Tue Jun 30 20:04:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Tyr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role bait bait physical association physical association Fig 5A (bottom) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. cdc20-cdc27 5 13 unspecified role unspecified role prey prey 19 unspecified role unspecified role ancillary ancillary Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 14:56:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187tyr' Tue Jun 30 17:17:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Tyr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig 5B (top) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. cdc27-cdc20-1 9 19 unspecified role unspecified role ancillary ancillary Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 19:44:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187tyr' Tue Jun 30 17:47:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Tyr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait 20 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 5B (bottom) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. fzr1-cdc27-7 5 13 unspecified role unspecified role prey prey P30260:p.Cys66Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain C R Wed May 02 18:59:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys66arg' Tue Jun 30 17:35:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys66Arg' 23 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9UM11:p.Lys69Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain K R Wed May 02 14:55:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys69arg' Tue Jun 30 17:20:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys69Arg' Q9UM11:p.Lys159Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:26:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys159arg' Tue Jun 30 17:52:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys159Arg' physical association physical association Fig 5C, 6A. siRNA-mediated knockdown of SIRT2 increased acetylation of CDH1 and CDC20 and decreased their interaction with CDC27. Mutations of the acetylation sites, K69R and K159R of CDH1 (Figure 6A), and K66R of CDC20 (Figure 6B), reduced the acetylation of these proteins and enhanced their interaction with CDC27. cdc27-cdc20-2 9 20 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role bait bait physical association physical association Fig 5D siRNA-mediated knockdown of SIRT2 increased acetylation of CDH1 and CDC20 and decreased their interaction with CDC27. fzr1-cdc27-10 4 15 unspecified role unspecified role ancillary ancillary 21 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 5E (LHS) SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). fzr1-cdc27-13 4 21 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 19 unspecified role unspecified role prey prey Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 16:51:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187tyr' Tue Jun 30 17:14:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His187Tyr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 5E (RHS) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). cdc20-fzr1 4 21 unspecified role unspecified role prey prey 15 unspecified role unspecified role ancillary ancillary 20 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 5F (LHS) SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). cdc20-fzr1-1 4 20 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 21 unspecified role unspecified role prey prey 15 unspecified role unspecified role ancillary ancillary region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5F (RHS) SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). aurka-aurka-1 3 16 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig S3C fzr1-sirt2-3 7 23 enzyme target enzyme target substrate substrate neutral component neutral component region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 11 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 16:04:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his150tyr' Tue Jun 30 19:32:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His150Tyr' direct interaction direct interaction Fig S4A (LHS) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. cdc20-sirt2-1 7 20 enzyme target enzyme target substrate substrate neutral component neutral component region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 11 enzyme enzyme neutral component neutral component Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 18:15:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his150tyr' Tue Jun 30 16:53:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His150Tyr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig S4A (RHS) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. cdc20-sirt2-2 8 20 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 14:25:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his150tyr' Tue Jun 30 19:39:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His150Tyr' physical association physical association Fig S4C The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. fzr1-sirt2-4 8 23 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 enzyme enzyme neutral component neutral component Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 19:44:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his150tyr' Tue Jun 30 20:13:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His150Tyr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig S4B The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. cdc20-cdc27-1 5 20 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey physical association physical association Fig 5D, 6B. siRNA-mediated knockdown of SIRT2 increased acetylation of CDH1 and CDC20 and decreased their interaction with CDC27. Mutations of the acetylation sites, K69R and K159R of CDH1 (Figure 6A), and K66R of CDC20 (Figure 6B), reduced the acetylation of these proteins and enhanced their interaction with CDC27.