IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. Cell (0092-8674) 2011 imex curation Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. Targeted crosslinking of the engineered cysteine residues in the N-terminal GTPase and C-terminal GED regions by 1,1-methanediyl bismethanethiosulfonate (MTS-1-MTS) produced a dimer, the less specific crosslinking of surface-reactive amines by BS3 primarily yields a tetramer. jennyh@helix.nih.gov, dyda@helix.nih.gov Accepted 2011-NOV-07 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. Cell (0092-8674) 2011 imex curation Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Accepted 2011-NOV-07 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. Cell (0092-8674) 2011 imex curation Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Accepted 2011-NOV-07 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. Cell (0092-8674) 2011 imex curation Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Accepted 2011-NOV-07 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. Cell (0092-8674) 2011 imex curation Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Accepted 2011-NOV-07 by MGT 21-Dec-2011: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. Cell (0092-8674) 2011 imex curation Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Accepted 2011-NOV-07 by MGT Cell (0092-8674) 2011 Only protein-protein interactions imex curation dyn1_human Dynamin-1 DNM1 DNM protein protein human Homo sapiens Human MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGMTKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKVAKEVDPQGQRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKIREPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPSRSGQASPSRPESPRPPFDL DIP protein Q05193 original sequence version: 142 DIP protein Q05193 original sequence version: 113 DIP protein Q05193 original sequence version: 100 DIP protein Q05193 original sequence version: 115 DIP protein Q05193-1 original sequence version: 111 DIP protein Q05193 original sequence version: 153 7FCD8CB572FFEAEF gcp guanosine 5'-[beta,gamma-methylene]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gdp-tetrafluoroaluminate transition-state analogue GDP.AlF4 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) GDP.AlF4 is a transition stsate analogue GDP.AlF4 is a transition stsate analogue gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 dyn1-1 6 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 13 16 direct interaction direct interaction Fig. 1 dnm1-gcp-4 2 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 8 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3, Table S2. 2.2A Rworking = 22.53% Rfree = 26.91% dnm1-gdp_tetrafluoro 5 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 9 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. Fig. 4A dnm1-gcp-3 4 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 9 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction Supp. Fig. 4B dnm1 1 7 unspecified role unspecified role neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain R C certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain R C Wed May 02 19:25:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg15cys,cys86ser,cys424ser,cys427ser,cys442ser,cys607ser,cys708ser,arg730cys' Tue Jun 30 16:40:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig. 6B dnm1-1 1 7 unspecified role unspecified role neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain R C certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain R C Wed May 02 18:18:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg15cys,cys86ser,cys424ser,cys427ser,cys442ser,cys607ser,cys708ser,arg730cys' Tue Jun 30 17:15:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig. 6B dnm1-gtp-1 3 10 enzyme target enzyme target substrate substrate neutral component neutral component 7 enzyme enzyme neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain R C certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain R C Wed May 02 17:38:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg15cys,cys86ser,cys424ser,cys427ser,cys442ser,cys607ser,cys708ser,arg730cys' Tue Jun 30 17:20:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys]' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig. S5 A/B DynRCL R15C/R730C reconstitutes WT dynamin's basal GTPase activity (A) and is stimulated by self-assembly in low ionic strength (B). dnm1-3 5 7 unspecified role unspecified role neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain R C certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain R C Wed May 02 20:18:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg15cys,cys86ser,cys424ser,cys427ser,cys442ser,cys607ser,cys708ser,arg730cys' Tue Jun 30 17:36:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys]' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supp. Fig. 5D, 5E Cross-linking with 1,1-methanediyl bismethanethiosulfonate (MTS-1-MTS) also produced a tetramer. dnm1-4 5 7 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. Fig. 5C