IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crystal Structure of the Mammalian GIRK2 K(+) Channel and Gating Regulation by G Proteins, PIP(2), and Sodium. Crystal Structure of the Mammalian GIRK2 K(+) Channel and Gating Regulation by G Proteins, PIP(2), and Sodium. Cell (0092-8674) 2011 imex curation Whorton M.R., Mackinnon R. mackinn@rockefeller.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Whorton M.R., Mackinnon R. mackinn@rockefeller.edu Accepted 2011-NOV-03 by MGT Cell (0092-8674) 2011 Only protein-protein interactions imex curation pip2-octanoylglyceryl L-ALPHA-D-MYOPHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE, D(+)SN1,2DI-O-OCTANOYLGLYCERYL small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) kcnj6_mouse G protein-activated inward rectifier potassium channel 2 Kcnj6 Girk2 Kcnj7 W Inward rectifier K(+) channel Kir3.2 Potassium channel, inwardly rectifying subfamily J member 6 protein protein mouse Mus musculus Mouse MTMAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQSDINVGYYTGDDRLFLVSPLIISHEINQQSPFWEISKAQLPKEELEIVVILEGIVEATGMTCQARSSYITSEILWGYRFTPVLTMEDGFYEVDYNSFHETYETSTPSLSAKELAELANRAEVPLSWSVSSKLNQHAELETEEEEKNPEELTERNGDVANLENESKV DIP protein P48542 original sequence version: 115 2E5153DCB1B60331 kcnj6-1 1 3 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Supp. Table 1 3.5A Rworking = 26% Rfree = 27.27% An Arg201ala mutant also crystallised as a tetramer (3SYC) kcnj6-c8-pip2 1 2 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Supp. Table 1 3.0A Rworking = 24% Rfree = 26.92% G proteins open a G loop gate in the cytoplasmic domain in the presence of PIP2. An Arg201ala mutant also crystallised as a tetramer (3SYQ). This shows a large change in both the G loop and the inner helix gates in comparison to wild tye and only two of the four subunits are free to bind PIP2 and undergo the conformational change